A compact short‐capillary based high‐speed capillary electrophoresis bioanalyzer

Fang, Xuefeng; Fang, Pan; Pan, Jian-Zhang; Fang, Qun

doi:10.1002/elps.201600195

Cited by 20 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A compact high‐speed CE bioanalyser based on a short capillary and presented previously was developed . The very short effective length (20 mm) of the 50 μm id capillary allows for the separation of FITC‐labelled Arg, Phe, Ala, Gly, Glu and Asp in less than 7 s. The peak height precision for these AAs ranged from 2.99 to 4.02%, while the LIF‐using LOD was approximately 0.4 nM.…”

Section: New Developments In Cementioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

View full text Add to dashboard Cite

Recent advances in amino acid analysis by capillary electromigration methods: June 2015-May 2017In the tenth edition of this article focused on recent advances in amino acid analysis using capillary electrophoresis, we describe the most important research articles published on this topic during the period from June 2015 to May 2017. This article follows the format of the previous articles published in Electrophoresis. The new developments in amino acid analysis with CE mainly describe improvements in CE associated with mass spectrometry. Focusing on applications, we mostly describe clinical works, although metabolomics studies are also very important. Finally, works focusing on amino acids in food and agricultural applications are also described.

show abstract

Section: New Developments In Cementioning

confidence: 99%

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

et al. 2017

View full text Add to dashboard Cite

show abstract

“…So far, two types of picoliter-scale sample injection techniques based on short capillaries 28 – 32 and microfluidic chips 34 – 36 have been developed. The former ones include optical-gating injection 28 , flow-gating injection 29 , 30 , and translational spontaneous injection 31 – 33 methods. The microchip-based injection methods include pinched injection 34 , 35 and gated injection 35 , 36 in cross channel or double-T channel.…”

Section: Resultsmentioning

confidence: 99%

“…In most of the hitherto reported miniaturized high-speed CE systems, microchips were adopted because they could provide the microchannel networks for achieving picoliter-scale sample injection and CE separation, although this may lead to the evident increase in system cost and operation complexity. Differing from the nanoliter-scale injection methods commonly used in conventional CE systems, the capillary-based picoliter-scale injection techniques 28 – 33 also provide an alternative strategy to achieve miniaturization of HSCE systems without the need of microfabricated chips. However, this strategy also brings difficulties in achieving system miniaturization since these systems need to use large-sized components, such as high-intensity laser for optical-gating injection 28 , flow switching device for flow-gating injection 29 , 30 , or mechanical translation stage for spontaneous injection 31 – 33 .…”

Section: Resultsmentioning

confidence: 99%

“…In the previous study in high-speed CE, the authors’ group has developed a translational spontaneous injection method for performing picoliter-scale sample injection in a short capillary with a switchable slotted-vial array 31 – 33 , 37 , 38 . The translational spontaneous sample injection was performed by first inserting the tapered inlet end of a separation capillary into a sample reservoir, and then allowing the sample reservoir to remove from the capillary at an angle of 90° between the reservoir moving direction and the capillary.…”

Section: Resultsmentioning

confidence: 99%

“…Actually, high-speed CE analysis can also be carried out alternatively on the basis of a short capillary by using special picoliter-scale injection methods such as optical gated injection 28 , flow gated injection 29 , 30 , and translational spontaneous injection 31 , 32 methods to maintain high separation efficiency. Recently, we developed a compact high-speed CE bioanalyzer integrated modules of sample injection and changing, short capillary-based CE separation, LIF detection, as well as a custom designed tablet computer for data processing, instrument controlling, and result displaying with a desktop instrument size of 230 × 170 × 190 mm 33 . Compared with microchip-based high-speed CE systems, the use of cheap and commercially available fused silica capillaries can reduce system cost and simplify system structure.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection

Pan

Fang

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

In this work, we developed a miniaturized palmtop high-speed capillary electrophoresis (CE) system integrating whole modules, including picoliter-scale sample injection, short capillary-based fast CE, high-voltage power supply, orthogonal laser induced fluorescence (LIF) detection, battery, system control, on-line data acquisition, processing, storage, and display modules. A strategy of minimalist miniaturization combining minimal system design and low-cost system construction was adopted to achieve the instrument miniaturization with extremely low cost, which is differing from the current microfabrication strategy used in most reported miniaturized CE systems. With such a strategy, the total size of the bioanalyzer was minimized to 90 × 75 × 77 mm (length × width × height) and the instrument cost was reduced to ca. $500, which demonstrated the smallest and lowest-cost CE instrument with LIF detection in so far reported systems. The present bioanalyzer also exhibited comparable analytical performances to previously-reported high-speed CE systems. A limit of detection of 1.02 nM sodium fluorescein was obtained. Fast separations were achieved for multiple types of samples as amino acids, amino acid enantiomers, DNA fragments, and proteins with high efficiency. We applied this instrument in colorectal cancer diagnosis for detecting KRAS mutation status by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Currently, miniaturization of analytical instruments with the aim of application in field analysis, point of care testing, environmental analysis and aerospace analysis, has become one of the main trends of analytical instrument researches. Since 1990s, the appearance of microfluidic technology has provided a strong impetus for analytical instrument miniaturization due to its advantages of high efficiency, high throughput, low consumption, as well as system miniaturization, integration, and automatization. By now, it has already become the major approach for achieving miniaturization of analytical instruments. Various microfluidic chip-based miniaturized analytical systems have been developed, including high-speed capillary electrophoresis (CE) 1-3 , microchip-based nucleic acid analysis 4,5 , centrifugal microfluidic immunoassay 6 and high-performance liquid chromatography 7 .High-speed CE which was first reported by Jorgenson's group in 1991 8 , is a type of CE technique with features of high separation speed and high separation efficiency over traditional CE technique 9 . A typical high-speed CE system usually can achieve fast sample separation within tens of seconds using short separation length (<15 cm), narrow injected sample plug (e.g. <100 μm), and high separation electric field strength (>500 V/cm), while keeping high separation efficiency up to micrometer or submicrometer plate heights. Various high-speed CE systems have been developed and applied in biological 10,11 , medical 12,13 , chemical 14,15 , and environmental 16,17 analysis. Currently, the miniaturization of h...

show abstract

A gravity‐independent single‐phase electrode reservoir for capillary electrophoresis applications

et al. 2023

View full text Add to dashboard Cite

Capillary electrophoresis (CE) holds great promise as an in situ analytical technique for a variety of applications. However, typical instrumentation operates with open reservoirs (e.g., vials) to accommodate reagents and samples, which is problematic for automated instruments designed for space or underwater applications that may be operated in various orientations. Microgravity conditions add an additional challenge due to the unpredictable position of the headspace (air layer above the liquid) in any two‐phase reservoir. One potential solution for these applications is to use a headspace‐free, flow‐through reservoir design that is sealed and connected to the necessary reagents and samples. Here, we demonstrate a flow‐through high‐voltage (HV) reservoir for CE that is compatible with automated in situ exploration needs, and which can be electrically isolated from its source fluidics (in order to prevent unwanted leakage current). We also demonstrate how the overall system can be rationally designed based on the operational parameters for CE to prevent electrolysis products generated at the electrode from entering the capillary and interfering with the CE separation. A reservoir was demonstrated with a 19 mm long, 1.8 mm inner diameter channel connecting the separation capillary and the HV electrode. Tests of these reservoirs integrated into a CE system show reproducible CE system operation with a variety of background electrolytes at voltages up to 25 kV. Rotation of the reservoirs, and the system, showed that their performance was independent of the direction of the gravity vector.

show abstract

A compact short‐capillary based high‐speed capillary electrophoresis bioanalyzer

Cited by 20 publications

References 46 publications

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

Recent advances in amino acid analysis by capillary electromigration methods: June 2015–May 2017

A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection

A gravity‐independent single‐phase electrode reservoir for capillary electrophoresis applications

Contact Info

Product

Resources

About