Background: The second leading deadliest disease affecting women worldwide, after lung cancer, is breast cancer. Traditional approaches for breast cancer diagnosis suffer from time consumption and some human errors in classification. To deal with this problems, many research works based on machine learning techniques are proposed. These approaches show their effectiveness in data classification in many fields, especially in healthcare. Methods: In this cross sectional study, we conducted a practical comparison between the most used machine learning algorithms in the literature. We applied kernel and linear support vector machines, random forest, decision tree, multi-layer perceptron, logistic regression, and k-nearest neighbors for breast cancer tumors classification. The used dataset is Wisconsin diagnosis Breast Cancer. Results: After comparing the machine learning algorithms efficiency, we noticed that multilayer perceptron and logistic regression gave the best results with an accuracy of 98% for breast cancer classification. Conclusion: Machine learning approaches are extensively used in medical prediction and decision support systems. This study showed that multilayer perceptron and logistic regression algorithms are performant ( good accuracy specificity and sensitivity) compared to the other evaluated algorithms.