A comparative analysis of detachment forces and energies in initial and mature cell-material interaction

Wysotzki, Philipp; Sancho, Ana; Gimsa, Jan; Groll, Jürgen

doi:10.1016/j.colsurfb.2020.110894

Cited by 19 publications

(22 citation statements)

References 47 publications

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“…The difference between both cell lines was most significant at a contact time of 20 s, with the work for Sox2 cells being almost two-fold (150 vs. 76 fJ). These values are in good agreement with previous findings in literature [37,38]. Further investigation of the curve shape was performed by fitting exponential decay functions to the recovery to zero-fore.…”

Section: Strength and Work Of Adhesion Differ For Mcf7 And Sox2 Overesupporting

confidence: 91%

Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

Iturri

Weber

Vivanco

et al. 2020

Cells

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The replacement of the cantilever tip by a living cell in Atomic Force Microscopy (AFM) experiments permits the direct quantification of cell–substrate and cell–cell adhesion forces. This single-cell probe force measurement technique, when complemented by microscopy, allows controlled manipulation of the cell with defined location at the area of interest. In this work, a setup based on two glass half-slides, a non-fouling one with bacterial S-layer protein SbpA from L. sphaericus CMM 2177 and the second with a fibronectin layer, has been employed to measure the adhesion of MCF7 breast cancer cells to fibronectin films (using SbpA as control) and to other cells (symmetric vs. asymmetric systems). The measurements aimed to characterize and compare the adhesion capacities of parental cells and cells overexpressing the embryonic transcription factor Sox2, which have a higher capacity for invasion and are more resistant to endocrine therapy in vivo. Together with the use of fluorescence techniques (epifluorescence, Total Internal Fluorescence Microscopy (TIRF)), the visualization of vinculin and actin distribution in cells in contact with fibronectin surfaces is enabled, facilitating the monitoring and quantification of the formation of adhesion complexes. These findings demonstrate the strength of this combined approach to assess and compare the adhesion properties of cell lines and to illustrate the heterogeneity of adhesive strength found in breast cancer cells.

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Section: Strength and Work Of Adhesion Differ For Mcf7 And Sox2 Overesupporting

confidence: 91%

Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

Iturri

Weber

Vivanco

et al. 2020

Cells

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“…FluidFM technologies have been shown to detect 2–3 orders of magnitude higher F max forces in dependence on cell–cell contacts when lifting single cells out of cell sheets as compared to SCFS and in comparison to subconfluent, single cells. 73 , 74 Therefore, it might be ideally suited to measure the strength of cell–cell contacts above and below the LCST after monolayer formation, and we could already provide a first evidence that cell–cell adhesive forces are PMS modulated for subconfluency here. As cadherines and not ZO-1 were shown to control MDCK II cell mechanics, it would also be worthwhile to study the cortical tension and area compressibility for PMS cultured MDCK II cells 69 and also for all stages of cell confluency to account for stiffness changes and tension homeostasis upon transition to crowding.…”

Section: Discussionmentioning

confidence: 81%

Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting

Kim

Witt

Oswald

et al. 2020

ACS Appl. Mater. Interfaces

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Stimuli responsive polymer coatings are a common motive for designing surfaces for cell biological applications. In the present study, we have characterized temperature dependent adhesive properties of poly( N -isopropylacrylamide) (PNIPAm) microgel coated surfaces (PMS) using various atomic force microscopy based approaches. We imaged and quantified the material properties of PMS upon a temperature switch using quantitative AFM imaging but also employed single-cell force spectroscopy (SCFS) before and after decreasing the temperature to assess the forces and work of initial adhesion between cells and PMS. We performed a detailed analysis of steps in the force–distance curves. Finally, we applied colloid probe atomic force microscopy (CP-AFM) to analyze the adhesive properties of two major components of the extracellular matrix to PMS under temperature control, namely collagen I and fibronectin. In combination with confocal imaging, we could show that these two ECM components differ in their detachment properties from PNIPAm microgel films upon cell harvesting, and thus gained a deeper understanding of cell-sheet maturation and harvesting process and the involved partial ECM dissolution.

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“…Furthermore, biochemical‐mediated immobilization techniques may perturb cells (Friedrichs et al, 2010). Using the fluidFM technique (Meister et al, 2009a), it is relatively easy to attach a cell by applying negative pressure in the microchannel of the micropipette and bring it into contact with other cells or functionalized surfaces to measure adhesion (Wysotzki et al, 2020). Importantly, fluidFM cantilevers can be reused.…”

Section: From Afm To Fluidfmmentioning

confidence: 99%

Extending applications of AFM to fluidic AFM in single living cell studies

Qiu

Chien

Maroulis

et al. 2022

Journal Cellular Physiology

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In this article, a review of a series of applications of atomic force microscopy (AFM) and fluidic Atomic Force Microscopy (fluidic AFM, hereafter fluidFM) in single-cell studies is presented. AFM applications involving single-cell and extracellular vesicle (EV) studies, colloidal force spectroscopy, and single-cell adhesion measurements are discussed. FluidFM is an offshoot of AFM that combines a microfluidic cantilever with AFM and has enabled the research community to conduct biological, pathological, and pharmacological studies on cells at the single-cell level in a liquid environment. In this review, capacities of fluidFM are discussed to illustrate (1) the speed with which sequential measurements of adhesion using coated colloid beads can be done, (2) the ability to assess lateral binding forces of endothelial or epithelial cells in a confluent cell monolayer in an appropriate physiological environment, and(3) the ease of measurement of vertical binding forces of intercellular adhesion between heterogeneous cells. Furthermore, key applications of fluidFM are reviewed regarding to EV absorption, manipulation of a single living cell by intracellular injection, sampling of cellular fluid from a single living cell, patch clamping, and mass measurements of a single living cell.

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A comparative analysis of detachment forces and energies in initial and mature cell-material interaction

Cited by 19 publications

References 47 publications

Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

Single-Cell Probe Force Studies to Identify Sox2 Overexpression-Promoted Cell Adhesion in MCF7 Breast Cancer Cells

Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting

Extending applications of AFM to fluidic AFM in single living cell studies

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