A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers

Shao, Qingsong; Q, Guo; Deng, Yanming; Guo, Haipeng

doi:10.1016/j.bse.2010.11.002

Cited by 63 publications

(49 citation statements)

References 24 publications

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“…Our results suggest that both ISSR and SRAP markers are effective and reliable. Briefly, Mantel tests showed that "r" was 0.864 (P = 0.9940), suggesting a high correlation between these two molecular techniques, which was in agreement with the results reported by Budak et al (2004) and Shao et al (2010). The results presented in this report could serve as a model for establishing an effective system for T. sinensis germplasm identification and genetic diversity analysis.…”

Section: Effectiveness Of Issr and Srap Marker For T Sinensissupporting

confidence: 89%

“…To date, there was only one report on T. sinensis genetic diversity study using RAPD and isoenzymes method . Currently, ISSR and SRAP markers have been widely used for DNA fingerprinting, genetic diversity analyses, and germplasm evaluation due to their high efficiency and low cost (Liu et al, 2008;Shao et al, 2010). To our knowledge, this is the first report on T. sinensis genetic diversity evaluation using both ISSR and SRAP marker systems.…”

Section: Effectiveness Of Issr and Srap Marker For T Sinensismentioning

confidence: 98%

“…Using random-amplified polymorphic DNA (RAPD) and isoenzyme approaches, Wang et al (2008) reported the low diversity of T. sinensis natural resources in China. Compared with RAPD, ISSR and SRAP have higher reproducibility and are more effective for genetic diversity analyses and germplasm evaluation (Shao et al, 2010;Medri et al, 2011;Niu et al, 2015;Liu et al, 2008Liu et al, , 2015Souza-Sobreira et al, 2015). Using these two marker systems, we studied the genetic diversity and genetic relationships of five T. sinensis populations (100 individuals/sample) representing different climates and geographical locations in China.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

et al. 2016

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ABSTRACT. Toona sinensis Roem has an important value as a type of traditional vegetable and Chinese medicinal herb, and is also a valuable source of wood in China. In this study, we used the inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers to assess the level and pattern of genetic diversity in five domesticated T. sinensis populations in China. Our results indicated a relatively low level of genetic diversity both at species (Hs = 0.1662, 0.2098, respectively) and population levels (Hs = 0.0978, 0.1145, respectively). Molecular variance analyses revealed a relatively high degree of differentiation among populations (G ST = 0.3901, 0.4498), and low levels of gene flow (N m = 0.7816 and 0.6116). We divided the five populations into two groups by cluster analysis: group one consists of populations collected from the south part of China (e.g., Yuxi, Yunan Province and Zuanjiang, Chongqing Municipality), and group two contains those cultivated in north part of China (e.g., Hengshui, Hebei Province, Jinan and Rizhao, Shandong Province). The correlation of genetic relationships among populations fits well with their geographical distribution (Mantel test; r = 0.7236 and 0.6789, respectively). Asexual propagation, limited gene flow and geographic isolation are most likely the key factors associated with the observed genetic structure of T. sinensis grown in China. The present study indicated that both ISSR and SRAP markers were effective and reliable for assessing the degree of T. sinensis genetic variations.

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Section: Effectiveness Of Issr and Srap Marker For T Sinensissupporting

confidence: 89%

Section: Effectiveness Of Issr and Srap Marker For T Sinensismentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

et al. 2016

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“…Budak et al (2004) and Fu et al (2008) reported that SRAP was more informative than ISSR for assessing genetic diversity, but Wang et al (2012) reported that ISSR was more effective than SRAP for assessing the genetic variation of goat's rues. Song et al (2010) and Shao et al (2010) observed that both markers were similarly effective. In this study, SRAP showed more polymorphic bands and lower derived genetic similarity than ISSR.…”

Section: Discussionmentioning

confidence: 91%

“…These studies have given useful clues to understand species or genera relationships that assist in implementing a sound breeding program. Recently, ISSR and SRAP have been used to characterize medicinal chrysanthemum (Shao et al, 2010), but no similar reports on genetic diversity among wild species of Chrysanthemum and related genera are available.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers

Zhang¹,

Zhang²,

Zhao³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In this study, inter-simple sequence repeats (ISSRs) and sequence-related amplified polymorphism (SRAP) were applied to assess the genetic diversity in 38 species of Chrysanthemum and related genera. A total of 204 and 567 bands were amplified by 24 ISSR and 25 SRAP primers, of which 196 (97%) and 557 (99%) were polymorphic, respectively. The ISSR-based genetic similarity ranged from 0.016 to 0.886 and averaged 0.201, while the SRAP-based genetic similarity varied from 0.010 to 0.811 and averaged 0.122. Both the ISSR and SRAP techniques revealed similar clustering patterns and grouped species of Chrysanthemum and Ajania together. The results of principal coordinate analysis corroborated the unweighted pair group method with arithmetic average clustering. Additionally, results from ISSR and SRAP data were significantly correlated (r = 0.89, P < 0.001). Knowledge about genetic diversity among species can aid the transfer of traits of interest from the wild into cultivated chrysanthemum in future distant interspecific breeding.

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Strigolactone pathway genes and plant architecture: association analysis and QTL detection for horticultural traits in chrysanthemum

et al. 2016

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Chrysanthemums are important ornamental plants with abundant phenotypic diversity. Especially in cut-flower breeding, shoot branching is important for the success of new varieties. To assess the genetic regulation of shoot branching and other horticultural important traits, we phenotyped and genotyped two types of chrysanthemum populations: a genotype collection of 86 varieties and a biparental F1-population (MK11/3) of 160 individuals. Using two different statistical approaches, a genome-wide association analysis and a single marker ANOVA, with AFLP marker data and candidate gene markers for shoot branching, we tried to identify markers correlated to the traits of interest. As expected for the outcrossing hexasomic chrysanthemums most of the phenotypic traits showed a continuous variation in both populations. With the candidate gene approach we identified 11 significantly associated marker alleles for all 4 strigolactone pathway genes BRC1, CCD7, CCD8 and MAX2 regulating shoot branching in the genotype collection. In the MK11/3 we detected seven markers for all candidate genes except MAX2 explaining a large proportion of the variation. Using anonymous AFLP markers in the GWA with the 86 genotypes and the single locus analysis with the F1-population we could detect 15 and 17 additional marker-trait associations, respectively. Our analyses indicate a polygenic inheritance of the shoot branching in the chrysanthemum, with a fundamental role of the strigolactone pathway genes BRC1, CCD7, CCD8 and MAX2 and we identified 50 associated markers to all traits under study. These markers could be used in the selection of the parental plants for breeding chrysanthemums to enrich them for positive alleles influencing plant architecture traits.

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A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers

Cited by 63 publications

References 24 publications

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

Comparative analysis of genetic diversity among species of Chrysanthemum and its related genera using inter-simple sequence repeat and sequence-related amplified polymorphism markers

Strigolactone pathway genes and plant architecture: association analysis and QTL detection for horticultural traits in chrysanthemum

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