A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

Castro, Isaura; Pinto-Carnide, Olinda; Ortiz, Jesús; Ferreira, Vanessa; Martín, Juan José Molina

doi:10.5424/sjar/2016144-8852

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…In this study, the RAPD primer OPA-02 revealed a high capacity for grapevine cultivar discrimination. The high number of polymorphic bands with the average percentage 67% was comparable to the results of Castro et al (2016). The average polymorphism among local cultivars was found to be 65.49%, using twenty-five primers.…”

Section: Molecular Analysissupporting

confidence: 78%

Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits

Abdelaziz

El-Sherif

2020

Bangladesh J. Plant Taxon

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Grapevine is one of the major fruit trees in the Kingdom of Saudi Arabia. This study aims to discriminate and assess the genetic diversity in three grapevine cultivars in Al- Madinah through the combination of characterization using both classical ampelographic as well as ampelometric studies with molecular markers using Randomly Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Start Codon Targeted Polymorphism (SCoT). For the ampelographic analysis, twelve OIV descriptors were used, and for the ampelometric analysis, the fully expanded mature leaves area were automatedly measured. The genetic distance among the three grapevines cultivars, calculated using Jaccard's coefficient, ranged from 0.7577 (between AL Nakheel and Banati) to 0.4501 (between AL Nakheel and Ahmer). The molecular analysis was based on the use of thirty-one primers; ten RAPD primers, seven ISSR primers and fourteen SCoT primers. RAPD primers generated the highest polymorphism (67%), while the level of polymorphism with ISSR primers was 36% and with SCoT 44%. All of the three markers generated similar dendrograms, and the genetic distance generated with RAPD was higher compared with SCoT and ISSR. The three markers RAPD, ISSR and SCoT were combined and amalgamated with the morphological data, and combined dendrogram was generated and discussed. AL Nakheel and Ahmer cultivars were found to be more closely related to each other than Banati which was separated in a different cluster. Both methods were effectively efficient for complete identification of grapevine and for studying the genetic diversity between closely related cultivars. Bangladesh J. Plant Taxon. 27(1): 113-127, 2020 (June)

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Section: Molecular Analysissupporting

confidence: 78%

Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits

Abdelaziz

El-Sherif

2020

Bangladesh J. Plant Taxon

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“…Similarly, 55 polymor phic bands were obtained in Palestine by Basheer Salimia, (2015) in studying 36 grape accessions using 17 ISSR markers; while 69 polymorphic bands were obtained by Seyedimoradi et al (2012) in studying 21 local Iranian grapevine cultivars using 10 ISSR primers. Our results related to polymorphism rate (5085%) and size of amplified bands (1251000bp) are close to the ones previously reported in Portugal (Castro et al, 2016), Turkey (Sabir et al, 2009), Egypt (Hassan et al, 2011), Iran (Seyedimoradi et al, 2012), and Palestine (BasheerSalimia, 2015. Larger frag ments (3001500 bp) were generated by the same ISSR markers with values up to 1500 bp in India (Dhanorkar et al, 2005) and 2500 bp in Turkey (Sabir et al, 2009).…”

Section: Discussionsupporting

confidence: 91%

“…In addition to the ampelograhic description, in order to discriminate the varieties, synonyms, homonyms and the variation among the accessions, molecular methods has become more frequently used especially markers based on Polymerase chain reaction techniques (Riaz et al, 2012;Madhumati, 2014). Among the molecular approaches, the ISSR (Inter Sequence Simple Repeat) has being evaluated for its usefulness in grapevine cultivar identification and in assessing genetic diversity of grapevine germplasms (Moreno et al, 1998;Dhanorkar et al, 2005;Santiago et al, 2005;This et al, 2006;Sabir et al, 2009;Seyedimoradi et al, 2012;Choudhary et al, 2014;Castro et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic and ampelographic characterization of grapevine accessions maintained in the Lebanese national collection

Chehade

Chalak²,

Merheb³

et al. 2022

Adv. Hort. Sci.

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Safeguarding grapevine biodiversity is one of the main concerns in viticulture today. Management of ex situ collections requires a comprehensive characterization of the conserved germplasm to provide basic material for selection and breeding programs. In this study, the local grapevine germplasm conserved at the national collection of the Lebanese Agricultural Research Institute and composed of 43 accessions was submitted to a genetic and ampelographic study. Nine ISSR primers, previously developed in grapevine, were used in this study. These primers generated a total of 51 bands, of which 77.7% were polymorphic allowing the differentiation of 41 genetic profiles vs. one case of synonymy that was recorded among three accessions. Ampelographic characterization was conducted using a set of 33 descriptors established by the International Office of Vine and Wine and related to leaf, bunch, and berry. Principal component analysis identified 12 descriptors i.e. veraison date, maturity date, berry length, upper and lower vein pigmentation, bunch density, bunch weight, sugar content at harvesting, flesh of juiciness, berry weight, flesh firmness, and color skin, as being the most discriminating descriptors. The correlation between the ISSR clustering and the ampelographic one was not significant (r=0.26) because of the divergence of accessions groups, except for the three accessions synonymy case which was confirmed in both dendrograms. Finally, this comprehensive evaluation of the existing local gene pool of grapevine revealed a substantial diversity. It would further allow the promotion of the valuable accessions directly through multiplication schemes, and their sustainable utilization in genetic improvement programs.

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“…Similarly, using RAPD and ISSR markers to assess genetic diversity among 40 cashew cultivars from India, Thimmappaiah et al (2016) found 163 bands, from which 129 were polymorphic, corresponding to 79.6% polymorphism. Castro et al (2016), using the same markers to study the genetic diversity among 56 grapevine cultivars, observed that 145 fragments were amplified, from which 116 were polymorphic bands, an 80% polymorphism. Thus, such results showed that both markers are effective in assessing genetic diversity.…”

Section: Genetic Diversitymentioning

confidence: 99%

Diversidad genética y fisicoquímica en doce cultivares brasileños de anacardo (Anacardium occidentale)

Lemos¹,

Bordallo²,

Neto³

et al. 2021

Acta Bot. Mex.

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Background and Aims: Cashew (Anacardium occidentale) is a native Brazilian tree, cultivated in various warm climates across the world. The cashew kernels production has been an important economic activity for many tropical countries, providing a variety of food with great nutrition value. The genetic diversity among the early dwarf cultivars used to production is poorly understood, as is the nutritional composition among nuts from those plants. This work aimed to evaluate the physicochemical characteristics of cashew kernels and to study genetic diversity among twelve cultivars to indicate superior genotypes for future breeding programs. Methods: Cashew kernels from twelve cultivars were evaluated for pH, titratable acidity, moisture, ash, lipids and proteins. The genetic variability was assessed using RAPD and ISSR molecular markers. Data from molecular and physicochemical analyses were used to estimate the genetic distances and Mantel test approach was applied to calculate Pearson’s correlation between the data.Key results: A high genetic variability was found among the cultivars. BRS 274 and BRS 275 were the closest genetically, while CCP 09 and BRS 189 were the most distant ones. On the other hand, EMBRAPA 51 and BRS 226 showed cashew kernels with more similar physicochemical characteristics, while BRS 189 and BRS 253 had the more different ones. Moreover, no correlation was found between the physicochemical and molecular results. Cashew kernels had pH close to neutrality, low acidity, considerable moisture content, and high lipid and protein contents. Conclusions: The cashew kernels from the cultivars studied here had low acidity and pH, considerable moisture and ash contents, and high protein and lipid contents. BRS 189, BRS 253, and CCP 1001 showed the best values for proteins, lipids, acidity, and pH. In addition, those cultivars have maximum distance genetic among them, which can be used as parents in future gene combinations in breeding programs.

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A comparative analysis of genetic diversity in Portuguese grape germplasm from ampelographic collections fit for quality wine production

Cited by 5 publications

References 30 publications

Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits

Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits

Genetic and ampelographic characterization of grapevine accessions maintained in the Lebanese national collection

Diversidad genética y fisicoquímica en doce cultivares brasileños de anacardo (Anacardium occidentale)

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