A comparative analysis of measles virus RNA by oligonucleotide fingerprinting

Stephenson, J. R.; Meulen, Volker ter

doi:10.1007/bf01315058

Cited by 9 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is assumed to be due to the secondary structure of the oligonucleotides affecting the relative efficiency of the kinase reaction. Similar observations have been noted when the genomes of several RNA viruses were analysed by this method (Frisby, 1977;Stephenson & ter Meulen, 1982). However, these variations in intensity were judged not to affect the validity of the conclusions since the fingerprint patterns were reproducible in repeated analyses of the same RNA sample and for different RNA preparations from the same virus stock.…”

Section: Resultssupporting

confidence: 68%

Oligonucleotide Fingerprint Analysis of Coxsackievirus A10 Isolated in Japan

Kamahora

Itagaki

Hattori

et al. 1985

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYEight coxsackievirus A10 strains isolated in 1978 and in 1981 and 1982 from patients with hand, foot-and-mouth disease and with herpangina at a dispensary in Matsue city were compared by RNA fingerprinting techniques. The oligonucleotide maps of the four 1978 isolates were related to each other by 85 to 93% with respect to their large T1 oligonucleotides. In contrast, the oligonucleotide maps of the four 1981 and 1982 isolates were very different from each other. Co-electrophoresis experiments revealed that the 1981 and 1982 strains shared only 17 to 34 % of their large oligonucleotides. In addition, some large oligonucleotides were found in most of the fingerprint maps of isolates from 1978 to 1982, suggesting that there are regions in the genome of coxsackievirus A10 which are not subject to mutational changes.

show abstract

Section: Resultssupporting

confidence: 68%

Oligonucleotide Fingerprint Analysis of Coxsackievirus A10 Isolated in Japan

Kamahora

Itagaki

Hattori

et al. 1985

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…We assumed this was due to formation of secondary structures among some of the T1-digested oligonucleotides, which affected the relative efficiency of the kinase reaction. Similar observations have been noted for other RNA viruses (Frisby 1977, Stephenson & Ter Meulen 1982, Kamahora et al 1985, Kusters et al 1987. The pres- ence of several small genetic subpopulations (a result of the high mutation rate of RNA viruses) in addition to the dominant population (quasispecies) may also be a factor (Steinhauer et al 1989b).…”

supporting

confidence: 56%

Genetic comparison of viral hemorrhagic septicemia virus isolates from North America and Europe

Oshima¹,

Higman²,

Arakawa³

et al. 1993

Dis. Aquat. Org.

View full text Add to dashboard Cite

Viral hemorrhagic septicemia virus (VHSV) is the cdusative agent of a serious rhabdoviral d~s e a s e of rainbow trout Oncorhynchus myklss in Europe The first isolation of the vlrus in North Amenca occurred In the fall of 1988 when it was recovered from adult chinook 0 tshawytscha and coho 0 klsutch salmon returning to 2 hatcher~es in the state of Washington, USA The following year, VHSV was isolated from adult coho salmon at 2 other hatcher~es in northwestern Washington In 1990 and 1991, VHSV was recovered from Pacific cod Gadus macrocephalus caught in Pnnce Willlam Sound, Alaska Genetic vanation among the 4 isolates from salmon and the 1990 ~solate from Pacific cod was d e t e r m~n e d uslng T1 nbonuclease finqerprlnt~ng In addition, 4 d~v e r s e isolates from Europe were lncluded for companson The North Amencan isolates of VHSV formed a slngle fingerprint group In which the 4 isolates from salmonids were h~g h l y similar to each other and the isolate from Pacific cod was related but less s~milar The 4 European ~solates which included an isolate from Atlantic cod G morhua, formed a second fingerpnnt group The genetic d~v e r s~t y among the isolates within each fingerpnnt group was estimated to be less than 5 % whlle the North Amencan and European strains of the virus were judged to differ by more than 5 % The results indicate that the North Amerlcan isolates of VHSV are not of European ongln and that the virus may be enzootic wlthin the m a n n e environment

show abstract

“…The high-molecularweight genome-length RNA was accompanied by heterogeneous subgenomic RNA (Fig. 3, lanes A and B), which was also observed in purified preparations of genomic RNA of measles virus (20,31) unless removed by extensive gradient centrifugation, nuclease treatment, or both (3). A second class of intracellular RNA consisted of monocistronic RNA which migrated faster than the 18S ribosomal RNA marker (Fig.…”

mentioning

confidence: 83%

“…To rule out a possible defect in the protein-coding function of the M cistron in this clone, the M cistron was excised from pcD-PM5d by digestion with Sall and BamHI and inserted into an in vitro expression vector which contained promoters derived from bacteriophages SP6 and T7 (pGEM-3) (Promega Biotec). The resulting clone, pSP6-PM5d-Aa, contained the complete M gene preceded by 31 30 ,ug of cloned DNA as previously described (35). At 30 h posttransfection, cells were starved for 30 min in methionine-free Eagle minimal essential medium and labeled with 30 ,uCi of [35S]methionine per culture.…”

mentioning

confidence: 99%

Structure and function of bicistronic RNA encoding the phosphoprotein and matrix protein of measles virus

Wong¹,

Hirano²

1987

J Virol

View full text Add to dashboard Cite

Two independent full-length replicas of a bicistronic RNA species containing the complete P and M genes of measles virus arranged in tandem were isolated from an expressible cDNA library. Sequences at the 5' and 3' termini suggested that the bicistronic RNA was initiated and terminated at precisely the same locations as the monocistronic mRNAs of the P and M genes, respectively. The P and M cistrons were fused together via an intergenic region which was exactly colinear with and complementary to the intergenic region of the genomic RNA. This RNA species was polyadenylated at the normal polyadenylation site at the 3' terminus of the M cistron, but not in the intergenic region. By DNA-mediated gene transfer, these cDNA clones were expressed into bicistronic RNA containing both P and M sequences in primate cells. RNA thus generated did not undergo nucleolytic processing but was translated into high levels of a 70,000AMr protein immunoprecipitated by monoclonal antiserum against the measles virus P protein. M protein was not produced in the same cells even though the M cistron could direct M protein synthesis in vitro once excised from the upstream P cistron. These results suggested that bicistronic RNA could direct protein synthesis from the first but not the second cistron and might contribute at least in part to expression of viral genes in vivo.

show abstract

A comparative analysis of measles virus RNA by oligonucleotide fingerprinting

Cited by 9 publications

References 24 publications

Oligonucleotide Fingerprint Analysis of Coxsackievirus A10 Isolated in Japan

Oligonucleotide Fingerprint Analysis of Coxsackievirus A10 Isolated in Japan

Genetic comparison of viral hemorrhagic septicemia virus isolates from North America and Europe

Structure and function of bicistronic RNA encoding the phosphoprotein and matrix protein of measles virus

Contact Info

Product

Resources

About