A comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells

Yun, Xinming; Huang, Qingchun; Rao, Wenbing; Xiao, Ciying; Zhang, Tao; Mao, Zhifan; Wan, Ziyi

doi:10.1016/j.ecoenv.2016.12.002

Cited by 37 publications

(19 citation statements)

References 39 publications

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“…EMB has been reported to maintain acute and longer-term toxicity (0.075 mg/kg for mice, oral) and cytotoxic effects of different types of mammalian cells across a widely spread of concentrations [ 7 , 24 , 25 ]. In this study, we have identified the EMB as a potential genotoxic compound corresponding to significant genotoxicity and cytotoxicity to human normal liver cells in vitro .…”

Section: Discussionmentioning

confidence: 99%

Potential genotoxic and cytotoxicity of emamectin benzoate in human normal liver cells

2017

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Pesticide residue inducing cancer-related health problems draw people more attention recently. Emamectin benzoate (EMB) has been widely used in agriculture around the world based on its specificity targets. Although potential risk and the molecular mechanism of EMB toxicity to human liver has not been well-characterized. Unlike well-reported toxicity upon central nervous system, potential genotoxic and cytotoxicity of EMB in human liver cell was ignored and very limited. In this study, we identify genotoxicity and cytotoxicity of EMB to human normal liver cells (QSG7701 cell line) in vitro. We demonstrate that EMB inhibited the viability of QSG7701 cells and induced the DNA damage. Established assays of cytotoxicity were performed to characterize the mechanism of EMB toxicity on QSG7701 cells. Typical chromatin condensation and DNA fragmentation indicated the apoptosis of QSG7701 cells induced by EMB. And the intracellular biochemical results demonstrated that EMB-enhanced apoptosis of QSG7701 cells concurrent with generated ROS, a loss of mitochondrial membrane potential, the cytochrome-c release, up regulate the Bax/Bcl-2 and the activation of caspase-9/-3. Our results of EMB induces the death of QSG7701 cells maybe via mitochondrial-mediated intrinsic apoptotic pathways would contribute to promote the awareness of EMB as an extensive used pesticide to human being effects and reveal the underlying mechanisms of potential genotoxic.

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Section: Discussionmentioning

confidence: 99%

Potential genotoxic and cytotoxicity of emamectin benzoate in human normal liver cells

2017

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“…Another plausible explanation is that we correctly identified chemicals that display some key characteristics of carcinogens, but that did not produce treatment-related tumors in the traditional rodent tests used to make cancer hazard classifications. Consistent with this explanation, recent studies have independently found that emamectin benzoate induces genotoxicity and cytotoxicity in vitro (Yun et al, 2017;Zhang et al, 2017), that triclosan activates cell migration pathways in vitro (Derouiche et al, 2017;Kim et al, 2015) and is a liver tumor promoter in mice (Yueh et al, 2014), and that didecyldimethylammonium chloride affects oxidative stress and cell growth in vitro (Kwon et al, 2014).…”

Section: Discussionmentioning

confidence: 78%

An Integrated Approach Using Publicly Available Resources for Identifying and Characterizing Chemicals of Potential Toxicity Concern: Proof-of-Concept With Chemicals That Affect Cancer Pathways

Iyer

Pham

Marty

et al. 2019

Toxicological Sciences

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We developed an integrated, modular approach to predicting chemical toxicity relying on in vitro assay data, linkage of molecular targets to disease categories, and software for ranking chemical activity and examining structural features (chemotypes). We evaluate our approach in a proof-of-concept exercise to identify and prioritize chemicals of potential carcinogenicity concern. We identified 137 cancer pathway-related assays from a subset of U.S. EPA's ToxCast platforms. We mapped these assays to key characteristics of carcinogens and found they collectively assess 5 of 10 characteristics. We ranked all 1061 chemicals screened in Phases I and II of ToxCast by their activity in the selected cancer pathway-related assays using Toxicological Prioritization Index software. More chemicals used as biologically active agents (eg, pharmaceuticals) ranked in the upper 50% versus lower 50%. Twenty-three chemotypes are enriched in the top 5% (n ¼ 54) of chemicals; these features may be important for their activity in cancer pathway-related assays. The biological coverage of the ToxCast assays related to cancer pathways is limited and short-term assays may not capture the biology of some key characteristics. Metabolism is also minimal in the assays. The ability of our approach to identify chemicals with cancer hazard is limited with the current input data, but we expect that our approach can be applied with future iterations of ToxCast and other data for improved chemical prioritization and characterization. The novel approach and proof-ofconcept exercise described here for ranking chemicals for potential carcinogenicity concern is modular, adaptable, and amenable to evolving data streams.

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“…Hepatocytes are increasingly being used as a cellular model for assessing the toxic potential of substances, as the liver is the principal organ associated with metabolic processes and xenobiotic toxicity [ 27 ]. Many insecticides have been tested on HepG2 cells to assess their effects on non-target organisms [ 28 , 29 ]. In our study, ACA was significantly less toxic to HepG2 cells than to Sf9 cells, consistent with what is expected in terms of selectivity for an insecticide molecule.…”

Section: Discussionmentioning

confidence: 99%

A Novel Insecticidal Molecule Extracted from Alpinia galanga with Potential to Control the Pest Insect Spodoptera frugiperda

Ruttanaphan

Sousa

Pengsook

et al. 2020

Insects

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Spodoptera frugiperda, a highly polyphagous insect pest from America, has recently invaded and widely spread throughout Africa and Asia. Effective and environmentally safe tools are needed for successful pest management of this invasive species. Natural molecules extracted from plants offer this possibility. Our study aimed to determine the insecticidal efficacy of a new molecule extracted from Alpinia galanga rhizome, the 1′S-1′-acetoxychavicol acetate (ACA). The toxicity of ACA was assessed by topical application on early third-instar larvae of S. frugiperda. Results showed that ACA caused significant larval growth inhibition and larval developmental abnormalities. In order to further explore the effects of this molecule, experiments have been performed at the cellular level using Sf9 model cells. ACA exhibited higher toxicity on Sf9 cells as compared to azadirachtin and was 38-fold less toxic on HepG2 cells. Inhibition of cell proliferation was observed at sublethal concentrations of ACA and was associated with cellular morphological changes and nuclear condensation. In addition, ACA induced caspase-3 activity. RT-qPCR experiments reveal that ACA induces the expression of several caspase genes. This first study on the effects of ACA on S. frugiperda larvae and cells provides evidence that ACA may have potential as a botanical insecticide for the control of S. frugiperda.

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A comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells

Cited by 37 publications

References 39 publications

Potential genotoxic and cytotoxicity of emamectin benzoate in human normal liver cells

Potential genotoxic and cytotoxicity of emamectin benzoate in human normal liver cells

An Integrated Approach Using Publicly Available Resources for Identifying and Characterizing Chemicals of Potential Toxicity Concern: Proof-of-Concept With Chemicals That Affect Cancer Pathways

A Novel Insecticidal Molecule Extracted from Alpinia galanga with Potential to Control the Pest Insect Spodoptera frugiperda

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