A Comparative Investigation of Peroxidases From Germinating Peanuts (Arachis Hypogaea): Electrophoresis

Abstract

Dormant seed and organs of 0‐, 1‐, 2‐, 5‐, 8‐, 11‐, and 14‐day‐old plants of Arachis hypogaea L. were homogenized in phosphate buffer and the lipid‐free extracts analyzed for benzidine and pyrogallol peroxidases using starch‐gel electrophoresis. On a wet weight basis, one weak band of benzidine peroxidase activity was detected in dormant cotyledons and three bands in 1‐day cotyledons. In 5‐day tissue, activity had increased significantly; at 14 days, the number of bands had decreased but staining intensity was maintained. In the extract from dormant axis, a single cathodic site of benzidine peroxidase activity was observed; however, on day two there was a marked increase in the number of bands and intensity of reaction in epicotyl and hypocotylradicle tissues. By day 14, the number and density of bands had decreased noticeably in the epicotyl and hypocotyl. Extracts from 14‐day roots exhibited more sites of reaction and greater intensity of staining of benzidine peroxidase than at five days of growth. Localized areas of activity at Rf ‐0.44 and ‐0.52 were present in extracts of all four organs when either benzidine or pyrogallol was used as the hydrogen donor. Although marked similarity existed between banding patterns of organs, qualitative and quantitative ontogenetic differences in peroxidases were apparent.

Peroxidases in Relation to Removal of Dormancy and Germination of Apple Embryos

“…Several authors have investigated the peroxidase activity and the isoperoxidase spectrum in embryos before their germination (Rychter and Lewak 1971, Thomas and Neucere 1974, Tao and Khan 1976, Gaspar et al, 1911, Their observations are in agreement with ours. The total peroxidase activity is always very low and results from some not very active isoperoxidases.…”

Seasonal Variation of Isozymes in Juniperus Scopulorum: Systematic Significance

“…The variation in isozyme patterns associated with growth and development in higher plants has been evaluated by using several approaches. Thomas and Neucere (1974) studied changes in peroxidases in peanut seeds and organs of various aged plants. They found a high similarity between banding patterns of different organs, but several qualitative and quantitative ontogenetic differences were detected.…”