A comparative proteomic study on the effects of metal pollution in oysters Crassostrea hongkongensis

Abstract

The metal pollution has posed great risk on the coastal organisms along the Jiulongjiang Estuary in South China. In this work, two-dimensional electrophoresis-based proteomics was applied to the oysters Crassostrea hongkongensis from metal pollution sites to characterize the proteomic responses to metal pollution. Metal accumulation and proteomic responses indicated that the oysters from BJ site were more severely contaminated than those from FG site. Compared with those oyster samples from the clean site (JZ), metal pollution induced cellular injuries, oxidative and immune stresses in oyster heapatopancreas from both BJ and FG sites via differential metabolic pathways. In addition, metal pollution in BJ site induced disturbance in energy and lipid metabolisms in oysters. Results indicated that cathepsin L and ferritin GF1 might be the biomarkers of As and Fe in oyster C. hongkongensis, respectively. This study demonstrates that proteomics is a useful tool for investigating biological effects induced by metal pollution.

The evolution of ependymin-related proteins

“…‘S ometsuke’ , an EPDR described in the abalone (a gastropod mollusc), has a role in shell biomineralisation and pigmentation [ 15 , 16 ], and EPDRs have also been found in mantle transcriptomes and shell proteomes in other mollusc species [ 17 – 19 ]. In addition, EPDRs have been implicated in gastropod larval development [ 20 ], are upregulated in response to toxins and metal pollution in bivalves [ 21 – 24 ], and are differentially regulated during pathogen challenge trials in bivalves [ 25 – 27 ] and in response to environmental stressors in both bivalves and gastropods [ 28 – 30 ]. Together, these studies are consistent with EPDRs having a diversity of functions, some of which appear to be lineage-specific.…”

Proteomic responses induced by metal pollutions in oysters Crassostrea sikamea

“…Interestingly, the 78 kDa glucose regulated protein was also up-regulated in another species of oyster C . hongkongensis sampled in Baijiao and Fugong sites severely contaminated by Cu (Xu et al, 2016). Besides BJ site, JS site was also heavily polluted by Cu, indicated by as high as 6 746.7 μg/g Cu (dry weight) concentrated in oysters (Ji et al, 2016).…”

“…sikamea were collected from three sites (Baijiao (BJ); Jinshan (JS); Jiuzhen (JZ)) along the Jiulongjiang estuary, Fujian Province, China. Our previous study showed that JZ site was a relatively clean site and therefore regarded as a reference (Xu et al, 2016). Comparatively, both JS and BJ sites were severely polluted by multiple metals (especially copper and zinc) (Weng and Wang, 2014;Tan et al, 2015;.…”

Oxidative Damage of Mussels Living in Seawater Enriched with Trace Metals, from the Viewpoint of Proteins Expression and Modification

“…It can also inactivate essential proteins by displacing Zn or Ca from the active sites [4], induce apoptosis and necrosis [17], and facilitate DNA mutagenesis through mismatch repair [18]. Finally, Hg is particularly prone to reacting with free-SH groups, inhibiting thereby the function of various proteins rich in thiols and depleting GSH levels [19]. Among trace metals, Hg is the most genotoxic agent, following the genotoxic potential order: Hg > Cu > Cd [13].…”