A Comparative Study Among Different Invasive Methods For The Diagnosis Of Helicobacter Pylori

Abstract

H. pylori infection occurs worldwide. Approximately 50% of the world population is infected with this organism. A cross-sectional study was conducted on 81 dyspeptic patients attending at Dhaka Medical College Hospital, for diagnostic endoscopy. H. pylori infection was measured by three invasive methods: culture, rapid urease test & histopathology. Among study patients maximum (34.57%) H. pylori infected were in 21-30 years age group, bearing male female ratio 1: 92.86. Forty four (54.32%) out of 81 were culture positive, 61 (75.31%) were rapid urease test positive and 62 (76.54%) were histopathology positive. By using 'gold standard' definition, 64 (79.02%) were H. pylori infected, 17 were un-infected. Finally comparing among three invasive methods, all are highly sensitive and specific to diagnose H. pylori infection.

Comparison of enzyme immunoassays detecting Helicobacter pylori specific IgG in serum and saliva with endoscopic and biopsy findings in patients with dyspepsia

“…[9] Furthermore, as suggested by some authors, such tests, can be considered as good as the gold standard biopsy test in diagnosing HP infection and screening of dyspeptic patients. [22,23] In settings similar to ours, we would therefore recommend the use of HP-specifi c salivary IgG assays, which offer a simple means of sample collection, especially in children and in diffi cult patients who usually resent venipuncture for blood sampling, although the issue of how best to diagnose HP infection remains a matter of concern [24] as do the many gray areas in the microbiological and clinical aspects of HP infection. [25] …”

Advances in diagnosis and treatment of Helicobacter pylori infection

“…Thus, there is a huge lack of single test as Gold Standard and optimal diagnostic technique. The only way to increase reliability of diagnosis is to apply for a multi-diagnostic tool [70,[73][74][75].…”

“…In addition, other reliable tests including stool assay and commercial diagnostic kits are available. However, there are some advantages and disadvantages with each one of the mentioned tools [68,71,74,82].…”

“…Ammonia vapor test is used for assessing the level of ammonia gas in the patient breath. This method is absolutely cost effective method [71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86][87][88].…”

Screening for Helicobacter pylori Infection among Asymptomatic University Students in Alexandria, Egypt, Using Non Invasive Laboratory Techniques

“…The only way to improve reliability of diagnosis is to apply for a multidiagnostic tool (Islam et al, 2010, Sethi et al, 2013 (Ranjbar et al, 2017). Inspite of the high sensitivity (88-95%) and specificity of UBT (95-100%) it has many limitations including its high cost, need of trained staff and equipped laboratory equipment, patients must fast before testing and proton pump inhibitor (PPI) administration before testing results in false negative results as it alters the gastric pH and thus lowers urease activity of H. pylori (Shimoyama, 2013).…”