A Comparative Study Between The Therapeutic Role of Adipose Derived Mesenchymal Stem Cells and Omeprazole in Regeneration of Gastric Ulcer Induced by Aspirin in Albino Rats : Histological and Immunohistochemical Study

Abstract

Background: Aspirin induced gastric mucosal injury, which leads to gastric ulcer. Adipose Derived Mesenchymal Stem Cells (ADMSC) can be used for treatment of gastric ulcer as they can differentiate into many cell types. Omeprazole is used for treatment of gastric acid-related disorders. The Aim of the Work: Was to compare between the therapeutic role of ADSMC and omeprazole in treatment of gastric ulcer Material and Methods: Forty male albino rats were divided into four equal groups: 1-Control group. 2-Aspirin group in which rats were fasted for 24 hours, then given acetylsalicylic acid (200 mg/kg) once orally for induction of ulcer. 3-ADMSCtreated group in which rats were treated by (3 × 10⁶) ADMSCs/ rat once by intravenous injection four hours after induction of ulcer. 4-Omeprazole-treated group in which rats were treated by omeprazole suspension (20 mg/kg) orally, four hours after induction of ulcer, and for five days. Gastric juice volume and acidity, length of gastric ulcer, and histopathological and immunohistochemical changes of the stomach were all evaluated. Results: Aspirin induced erosion and loss of architecture of gastric mucosal epithelium, ADMSC treatment reduced the length of gastric ulcer, ameliorate histopathological changes and reduced the PCNA-LI also the omeprazole treatment promoted healing of the gastric ulcer induced by aspirin but the therapeutic role of ADMSC was significantly lower in ulcer index and PCNA-LI and better in PAS reaction and histopathological healing of gastric ulcer than the therapeutic role of omeprazole. Conclusion:The therapeutic role of ADMSC was found to be superior to omeprazole in treatment of gastric ulcer.

Combined effect of pantoprazole and mesenchymal stem cells on experimentally induced gastric ulcer: implication of oxidative stress, inflammation and apoptosis pathways

“…In the current study, the control group revealed weak PCNA immunohistochemical reaction of the nuclei of the epithelial cells of gastric tissue of rats while the expression of immunohistochemically PCNA-stained section of the gastric tissue of rat from the GU group showed many PCNA positive nuclei of the epithelial cells of the gastric tissue. This result is consistent with recent studies showing that the gastric mucosa has strong immunoreactivity to PCNA dispersed throughout all gastric mucosal glandular (Alazzouni et al 2020 ) and epithelial cells (El-Kerdasy and Mousa 2021 ). The intense immunoreactivity to PCNA in the gastric tissue of rat in the GU group can be described as PCNA is a major indicator of tissue proliferation that occurs immediately after GU induction.…”

“…Concerning the immunohistochemical staining of PCNA in the gastric tissue of rats in the ADSCs-treated group, it revealed mild positive nuclei in the epithelial cells. This finding comes in line with El-Kerdasy and Mousa ( 2021 ) who reported that the ADSCs-treated group showed mild PCNA expression in the neck cells as compared with the aspirin-induced GU group as indicated in the immunohistochemical examination. MSCs enable to rebuild injured tissue and normalize the proliferative process following re-epithelialization, differentiation, and success in GU healing (Alazzouni et al 2020 ).…”

Therapeutic Versus Preventative Use of Ginkgo biloba Extract (EGb 761) against Indomethacin-Induced Gastric Ulcer in Mice

“…The PCNA expression was substantially higher in cases of gastritis and helicobacter pylori infection [ 14 ]. Its immunostaining reactivity was also dramatically increased in gastric ulcers induced by aspirin, which was considerably lower in the omeprazole-treated group [ 84 ]. Increased PCNA expression may be related to changes in cellular proliferation, which occurs as a compensatory reaction to mucosal injury.…”

Application of Immunohistochemistry in Rat Models of Erosive Gastritis

“…Euthanasia of animals and obtainment of gastric tissues is done 4-8 hours later [18][19][20][21] .In a study conducted by Elkerdasy et al, a different dosing regimen was employed to induce gastritis using acetylsalicylic acid (200 mg/kg). The rats, which were food-deprived overnight, received a single oral dose of 200 mg/kg of acetylsalicylic acid.…”

“…The rats, which were food-deprived overnight, received a single oral dose of 200 mg/kg of acetylsalicylic acid. This dosage was administered once [21]. In a study by Abdel-Kawi et al, 25 mg/kg body weight was administered intraperitoneally [22] .…”