A comparative study of antibody expressions in primary biliary cirrhosis and autoimmune cholangitis using phage display

Presence of antimitochondrial autoantibodies in patients with autoimmune hepatitis

“…[21][22][23][24][25][26][27] Thus, the presence of 'AMA-negative PBC' patients leads us to the suggestion that the existence of AMA, at least in sera, does not affect clinical, histological, or immunological characteristics of PBC. 27,28 Similarly, we demonstrated in the current study that no clinical or immunological difference was found between AMA-positive and AMA-negative patients with AIH. Taken together, it is can be assumed that the presence of serum AMA are not associated with, or at least are not sufficient for, generation of bile duct changes in both PBC and AIH livers.…”

Antimitochondrial Antibody-Negative Primary Biliary Cirrhosis

“…This suggests, assuming that the patients truly were AMA negative, a common pathogenetic pathway, with a variation in disease expression serologically. In this regard, a report looking at the serologic specificity of AMA-positive and AMA-negative PBC by antibody screening of phage-displayed random peptide libraries [30] identified a single immunodominant B-cell epitope related to the inner lipoyl domain of PDC-E2 that was independent of AMA status.…”

Unravelling autoimmune pathogenesis by screening random peptide libraries with human sera

“…By using the same technology a single conformational (discontinuous) epitope in the inner lipoyl domain of the E2 subunit of PDC was identified as the characteristic autoantigen in PBC. Biopanning a RPL with either PBC or AIC-derived IgG yielded phagotopes reacting with anti-PDC-E2 by capture ELISA, further establishing a similar autoimmune targeting in both diseases [80].…”