A comparative study of cellular and molecular pharmacology of doxorubicin and MEN 10755, a disaccharide analogue11Abbreviations: DOX, doxorubicin; DNA-SSB, single-strand breaks; and DNA-DSB, double-strand breaks.

Bigioni, Mario; Salvatore, Carmela; Bullo, Angela; Bellarosa, Daniela; Iafrate, Elisabetta; Animati, Fabio; Capranico, Giovanni; Goso, Cristina; Maggi, Carlo Alberto; Pratesi, Graziella; Zunino, Franco; Manzini, Stefano

doi:10.1016/s0006-2952(01)00645-1

Cited by 18 publications

(5 citation statements)

References 12 publications

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“…It is widely known that DOX is a kind of antitumor antibiotic that restrains the synthesis of nucleic acids such as DNA and RNA in the nucleoli of cancer cells, 28 while PTX binds to the Nterminal 31 amino acids of the beta-tubulin subunits of microtubules in the cytoplasm. 29 Gustafson et al studied the mechanism of the synergistic effect of DOX and TAX in vivo.…”

Section: Resultsmentioning

confidence: 99%

Dendrimer-based multilayer nanocarrier for potential synergistic paclitaxel–doxorubicin combination drug delivery

Wang

Wen

et al. 2014

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Dendrimer-based multilayer nanocarrier for potential synergistic paclitaxel–doxorubicin combination drug delivery

Wang

Wen

et al. 2014

RSC Adv.

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“…The accumulation assay was performed as previously described39 with several modifications as reported below. Exponentially growing SK‐N‐SH neuroblastoma and MCF7 ADR cells were plated in 25 cm 2 dishes at 5×10 5 cells per dish.…”

Section: Methodsmentioning

confidence: 99%

Interaction of the σ₂ Receptor Ligand PB28 with the Human Nucleosome: Computational and Experimental Probes of Interaction with the H2A/H2B Dimer

et al. 2010

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Sigma-2 (sigma(2)) binding sites are an emerging target for anti-neoplastic agents due to the strong apoptotic effect exhibited by sigma(2) agonists in vitro and the overexpression of these sites in tumor cells. Nonetheless, no sigma(2) receptor protein has been identified. Affinity chromatography using the high-affinity sigma(2) ligand PB28 and human SK-N-SH neuroblastoma cells was previously utilized to identify sigma(2) ligand binding proteins, specifically histones H1, H2A, H2B, and H3.3a. To rationalize this finding, homology modeling and automated docking studies were employed to probe intermolecular interactions between PB28 and human nucleosomal proteins. These studies predicted interaction of PB28 with the H2A/H2B dimer at a series of sites previously found to be implicated in chromatin compaction and nucleosomal assembly. To experimentally verify this prediction, a competitive binding assay was performed on the reconstituted H2A/H2B dimer using [(3)H]PB28 as radioligand, and an IC(50) value of 0.50 nM was determined for PB28 binding. In addition, [(3)H]PB28 was found to accumulate with up to a fivefold excess in nuclear fractions over cytosolic fractions of SK-N-SH and MCF7 cells, indicating that PB28 is capable of entering the nucleus to interact with histone proteins. In conjunction with computational results, these data suggest that PB28 may exert its cytotoxic effect through direct interaction with nuclear material.

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“…This aggregation phenomenon has also been linked to DNA cleavage experiments, during which reduced DNA damage was observed at high concentrations of DOX. 200 The aggregation prevents TOP2 access to DNA, leading to the formation of fewer cleavable complexes. These morphological insights provide a better understanding of the interplay between pharmacologic effect and structural dependence, an important consideration in therapeutic evaluation.…”

Section: Applications For Afm Imaging Of Dna Complexes In Cancer Researchmentioning

confidence: 99%

Atomic force microscopy—A tool for structural and translational DNA research

et al. 2021

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Atomic force microscopy (AFM) is a powerful imaging technique that allows for structural characterization of single biomolecules with nanoscale resolution. AFM has a unique capability to image biological molecules in their native states under physiological conditions without the need for labeling or averaging. DNA has been extensively imaged with AFM from early single-molecule studies of conformational diversity in plasmids, to recent examinations of intramolecular variation between groove depths within an individual DNA molecule. The ability to image dynamic biological interactions in situ has also allowed for the interaction of various proteins and therapeutic ligands with DNA to be evaluated—providing insights into structural assembly, flexibility, and movement. This review provides an overview of how innovation and optimization in AFM imaging have advanced our understanding of DNA structure, mechanics, and interactions. These include studies of the secondary and tertiary structure of DNA, including how these are affected by its interactions with proteins. The broader role of AFM as a tool in translational cancer research is also explored through its use in imaging DNA with key chemotherapeutic ligands, including those currently employed in clinical practice.

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A comparative study of cellular and molecular pharmacology of doxorubicin and MEN 10755, a disaccharide analogue11Abbreviations: DOX, doxorubicin; DNA-SSB, single-strand breaks; and DNA-DSB, double-strand breaks.

Cited by 18 publications

References 12 publications

Dendrimer-based multilayer nanocarrier for potential synergistic paclitaxel–doxorubicin combination drug delivery

Dendrimer-based multilayer nanocarrier for potential synergistic paclitaxel–doxorubicin combination drug delivery

Interaction of the σ₂ Receptor Ligand PB28 with the Human Nucleosome: Computational and Experimental Probes of Interaction with the H2A/H2B Dimer

Atomic force microscopy—A tool for structural and translational DNA research

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A comparative study of cellular and molecular pharmacology of doxorubicin and MEN 10755, a disaccharide analogue11Abbreviations: DOX, doxorubicin; DNA-SSB, single-strand breaks; and DNA-DSB, double-strand breaks.

Cited by 18 publications

References 12 publications

Dendrimer-based multilayer nanocarrier for potential synergistic paclitaxel–doxorubicin combination drug delivery

Dendrimer-based multilayer nanocarrier for potential synergistic paclitaxel–doxorubicin combination drug delivery

Interaction of the σ2 Receptor Ligand PB28 with the Human Nucleosome: Computational and Experimental Probes of Interaction with the H2A/H2B Dimer

Atomic force microscopy—A tool for structural and translational DNA research

Contact Info

Product

Resources

About

Interaction of the σ₂ Receptor Ligand PB28 with the Human Nucleosome: Computational and Experimental Probes of Interaction with the H2A/H2B Dimer