A comparative study of cultural methods for the detection of Salmonella in feed and feed ingredients

Abstract

Background: Animal feed as a source of infection to food producing animals is much debated. In order to increase our present knowledge about possible feed transmission it is important to know that the present isolation methods for Salmonella are reliable also for feed materials.

Accuracy and Sensitivity of Commercial PCR-Based Methods for Detection of Salmonella enterica in Feed

“…While the EFSA reported that the prevalence of Salmonella in other feed raw materials, e.g., grain, was significantly lower (6), Koyuncu and Haggblom observed that the sensitivity of cultural detection methods varied with the type of ingredient tested (30). Data collected here with PCR-based methods confirm the observations of Koyuncu and Haggblom (Fig.…”

“…Therefore, data from the various serotypes were pooled. In an earlier study using culture-based detection methods, we observed that even though feed ingredients were spiked with identical Salmonella cell numbers, detection limits varied with ingredient type (30). Similar results were obtained when PCR-based methods were employed in this study.…”

“…For spiking experiments, the batches of wheat grain, soybean meal, rape seed meal, palm kernel meal, pellets of pig feed, and scrapings from a feed mill were the same as those used in a previous study by Koyuncu and Haggblom (30). The feed materials were kept at 4°C (water activity, Ͻ0.7) until used and analyzed for Salmonella prior to the experiments as previously described (30).…”

Prevalence, Numbers and Antimicrobial Susceptibilities of Salmonella Serovars and Campylobacter spp. in Retail Poultry in Phnom Penh, Cambodia

“…Isolation and identification of Salmonella: Culture and isolation of Salmonella was conducted using standard method ISO 6579 as previously described [11]. Transparent well-isolated colonies with black center typical Salmonella morphology were collected from selective media and identified by using biochemical reactions.…”

Production of Soybean-Derived Feed Material Free from Salmonella Contamination: An Essential Food Safety Challenge

“…The same methods are principally also applied in the food industry and on samples from different environmental as well as clinical sources. Modified semisolid rappaport vassiliadis (MSRV)-based methods have been shown to be suitable for testing of feed [22]. An official authorization also of their use for feed and foodstuffs as well as for samples from primary food animal production would offer significant benefits in terms of harmonization across the food chain.…”