A comparative study of epidermal tight junction proteins in a dog model of atopic dermatitis

Abstract

ZO-1 and occludin, which have not been described to be associated with the development of AD in humans, could play a role in this atopic dog model. Further investigation on the expression and modulation of TJ proteins and their clinical relevance is needed.

Expression of ZO‐1 and claudin‐1 in a 3D epidermal equivalent using canine progenitor epidermal keratinocytes

“…The staining pattern and location of ZO‐1 in the epidermal equivalent were similar to those in the normal canine skin, as in previous reports . In prior studies of dogs with cAD, the expression of ZO‐1 was decreased in nonlesional skin compared with normal skin, similar to human AD . Several studies have demonstrated that interleukin‐1β and interleukin‐17 down‐regulated the expression of ZO‐1 in human keratinocytes .…”

“…In canine atopic dermatitis (cAD), the expression of claudin‐1 was reduced in nonlesional skin, whereas no significant difference was observed in ZO‐1 and occludin . In another study, the intensity of ZO‐1 expression was decreased in the nonlesional skin of atopic dogs, whereas those of claudin‐1 and occludin were not significantly different . These findings suggest that the expression of tight junctions is impaired; however, the specific roles of these tight junctions have not yet been clarified in cAD.…”

Calycosin Suppresses Epithelial Derived Initiative Key Factors and Maintains Epithelial Barrier in Allergic Inflammation via TLR4 Mediated NF-κB Pathway

“…The mRNA transcriptions and protein expressions of TJ-associated proteins (occluding and ZO-1) significantly reduce following epithelial barrier injury [35]. In an experimental AD model of dogs, TJs (mostly ZO-1 and occludin) are defective [14]. In this study, TEM and Western blot showed that TJs were dysregulated in the initial stage AD model of mice.…”

“…Moreover, disturbed skin barrier function such as that caused by TJs significantly increases the risks of AD and atopic diathesis [12, 13]. Kim et al reported that the expressions of occludin and ZO-1 were decreased in the atopic group by immunohistochemistry in experimental AD model in dogs [14]. …”

A review of the roles of keratinocyte‐derived cytokines and chemokines in the pathogenesis of atopic dermatitis in humans and dogs

“…22 In another study of cAD, immunohistochemical analyses demonstrated decreased expression in zonula occludens-1 and occludin, but not claudin-1. 23 Although there have been no studies investigating the expression levels of proteins at tight junctions in naturally occurring cAD, previous results from experimental cAD models suggest that dysfunction of the second physical barrier may also exist in cAD, similar to human AD.…”