Expression of ZO‐1 and claudin‐1 in a 3D epidermal equivalent using canine progenitor epidermal keratinocytes

Teramoto, Keiji; Asahina, Ryota; Nishida, Hidetaka; Kamishina, Hiroaki; Maeda, Sadatoshi

doi:10.1111/vde.12655

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Tight junctions and the stratum corneum have a synergistic effect on the formation of a strong skin barrier [24]. In the 3D skin equivalents, ZO-1 and CLDN-1 were expressed throughout the epidermal layer [25]. Similarly, our results by immunofluorescence staining analysis showed the presence of relevant barrier proteins as well as tight junction proteins.…”

Section: Biological Reactivity Of the Self-assembled Rse Modelsupporting

confidence: 72%

Structural and Functional Validation of a Full-Thickness Self-Assembled Skin Equivalent for Disease Modeling

et al. 2022

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Recently, various types of in vitro-reconstructed 3D skin models have been developed for drug testing and disease modeling. Herein, we structurally and functionally validated a self-assembled reconstructed skin equivalent (RSE) and developed an IL-17a-induced in vitro psoriasis-like model using a self-assembled RSE. The tissue engineering approach was used to construct the self-assembled RSE. The dermal layer was generated using fibroblasts secreting their own ECM, and the epidermal layer was reconstructed by seeding keratinocytes on the dermal layer. To generate the psoriatic model, IL-17A was added to the culture medium during the air–liquid interface culture period. Self-assembled RSE resulted in a fully differentiated epidermal layer, a well-established basement membrane, and dermal collagen deposition. In addition, self-assembled RSE was tested for 20 reference chemicals according to the Performance Standard of OECD TG439 and showed overall sensitivity, specificity, and accuracy of 100%, 90%, and 95%, respectively. The IL-17a-treated psoriatic RSE model exhibited psoriatic epidermal characteristics, such as epidermal hyperproliferation, parakeratosis, and increased expression of KRT6, KRT17, hBD2, and S100A9. Thus, our results suggest that a self-assembled RSE that structurally and functionally mimics the human skin has a great potential for testing various drugs or cosmetic ingredients and modeling inflammatory skin diseases.

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Section: Biological Reactivity Of the Self-assembled Rse Modelsupporting

confidence: 72%

Structural and Functional Validation of a Full-Thickness Self-Assembled Skin Equivalent for Disease Modeling

et al. 2022

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“…Similarly, in human AD, claudin-1 has been shown to be decreased and potentially associated with the pathogenesis of AD (De Benedetto et al, 2011). In a canine study, Teramoto et al (2018) used CPEK cells to investigate the expression of ZO-1 and claudin-1 in vitro, constructing a 3D skin-equivalent model as a research tool using CPEK cells. In this 3D model, keratinocytes differentiate to form a multilayer model of skin containing the upper layers of the differentiated epidermis, where tight junctions are expressed.…”

Section: Canine Progenitor Epidermal Keratinocytesmentioning

confidence: 99%

“…Impairment of tight junctions has been associated with the pathogenesis of canine AD (Teramoto et al., 2018). Similarly, in human AD, claudin‐1 has been shown to be decreased and potentially associated with the pathogenesis of AD (De Benedetto et al., 2011).…”

Section: In Vitro Inflammatory Skin Disease Modelsmentioning

confidence: 99%

Canine Models of Inflammatory Skin Diseases and Their Application in Pharmacological Research

Gil,

Santoro

2023

Current Protocols

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The purpose of this article is to provide an overview of existing pharmacological models of canine dermatitis. Canine models of dermatitis have contributed significantly to our current understanding of the pathology of dermatitis and to the development of corresponding pharmacological interventions. Specifically, canine atopic dermatitis (AD) is reviewed here, as it is one of the most common inflammatory skin diseases in dogs. Canine AD also shares clinicopathological features with human AD, making the dog a natural and optimal model for human disease. Thus, pharmacological models of canine AD may be uniquely applicable to human pharmacological research. In this article, particular attention is dedicated to relevant in vivo, in vitro, and ex vivo models of canine AD, skin barrier defect models, pruritus models, and skin immunology models. Additionally, models of superficial pyoderma and food allergy are also discussed. With understanding of findings from canine models, researchers can select the most salient features for future pharmacological drug development. © 2023 Wiley Periodicals LLC.

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“…Canine 3D organotypic skin models were obtained using canine progenitor epidermal keratinocytes onto an acellular collagen gel layer. Two studies based on this protocol produced canine epidermal equivalent with a well-defined cornified layer [147,148]. Keratinocytes derived iPSC [149] and bulge cellsenriched keratinocytes from dog hair-follicles [150] have also been used in reconstructed equine and canine epidermis, respectively.…”

Section: Skin Equivalents In Animalsmentioning

confidence: 99%

3D skin models in domestic animals

Souci

Denesvre

2021

Vet Res

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The skin is a passive and active barrier which protects the body from the environment. Its health is essential for the accomplishment of this role. Since several decades, the skin has aroused a strong interest in various fields (for e.g. cell biology, medicine, toxicology, cosmetology, and pharmacology). In contrast to other organs, 3D models were mostly and directly elaborated in humans due to its architectural simplicity and easy accessibility. The development of these models benefited from the societal pressure to reduce animal experiments. In this review, we first describe human and mouse skin structure and the major differences with other mammals and birds. Next, we describe the different 3D human skin models and their main applications. Finally, we review the available models for domestic animals and discuss the current and potential applications.

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Expression of ZO‐1 and claudin‐1 in a 3D epidermal equivalent using canine progenitor epidermal keratinocytes

Abstract: The CPEK 3D epidermal equivalent has the potential to be a suitable in vitro research tool for clarifying the specific role of tight junctions in cAD.

Cited by 6 publications

References 20 publications

Structural and Functional Validation of a Full-Thickness Self-Assembled Skin Equivalent for Disease Modeling

Structural and Functional Validation of a Full-Thickness Self-Assembled Skin Equivalent for Disease Modeling

Canine Models of Inflammatory Skin Diseases and Their Application in Pharmacological Research

3D skin models in domestic animals

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