A comparative study of methods for the diagnosis of respiratory virus infections in childhood

Abstract

SUMMARYNasopharyngeal secretions and cough/nasal swabs were taken from 111 children admitted to hospital in Newcastle upon Tyne with acute lower respiratory disease. A comparison was made between nasopharyngeal secretions and cough/nasal swabs as material for isolation of viruses in tissue culture. These results were, in turn, compared with those obtained by applying a fluorescent antibody technique to the exfoliated cells in the nasopharyngeal secretions for the rapid diagnosis of respiratory syncytial virus infection.More viruses were isolated in tissue culture from nasopharyngeal secretion than from cough/nasal swabs. Further evidence for the superiority of nasopharyngeal secretions was obtained by comparing the virus isolations in the laboratory in 1967 with those in 1968. Respiratory syncytial virus was not only isolated more often but more quickly in tissue culture inoculated with nasopharyngeal secretions.The fluorescent antibody technique not only provided a diagnosis on the patient's day of admission in 95% of those infected with respiratory syncytial virus but also proved to be as sensitive as the culture of nasopharyngeal secretions and considerably more sensitive than the culture of cough/nasal swabs for the diagnosis of respiratory syncytial virus infection.We wish to acknowledge the invaluable help given us by consultants, medical officers, ward sisters and their staff in the paediatric wards of the Child Health Department. We also wish to thank the technical staff in the Department of Virology for their aid.

The local antibody response to R.S. virus infection in the respiratory tract

“…Whenever possible, a second secretion was obtained from the patient after an interval of approximately 7 days. The method of collection and preparation of secretions has been fully described elsewhere (Sturdy, McQuillin & Gardner, 1969;Scott & Gardner, 1970).…”

Bovine-Testis Cells for Routine Isolation of Respiratory Syncytial Virus From Infants

“…Because of very rapid multiplication, cells from the first five passages would have been Although cough swabs and not nasopharyngeal secretions were used in these earlier studies, the use of secretions would be expected to increase the isolation rate by only about 5% (Sturdy et al, 1969). We concluded that the HL cells used in our comparison were probably of maximal sensitivity.…”

Observations on Clinical and Immunofluorescent Diagnosis of Parainfluenza Virus Infections

“…This communication covers the period from February 1968 to November 1970. The clinical categories used and the precise method of obtaining both nasopharyngeal secretions and cough/nasal swabs have been described (Gardner et al, 1960;Sturdy et al, 1969), and the immunofluorescent techniques are also identical with those used in our earlier studies McQuillin and McQuillin et al, 1970). During the period when the occurrence of parainfluenza virus infections were most frequent, between September 1969 and November 1970, all specimens suitable for examination by immunofluorescence were investigated for parainfluenza virus types 1, 2, and 3 when appropriate.…”