A comparative study of specificity of fucoidanases from marine microorganisms and invertebrates

Abstract

Specificities of actions of fucoidanases from the marine microorganism Pseudoalteromonas citrea KMM 3296 and the marine mollusk Littorina kurila were studied. The enzymes possess similar specificities and catalyze the cleavage of accessible α-(1→3)-fucoside bonds in fucoidans with highly sulfated α-(1→4; 1→3)-L-fucooligosaccharides. A high degree of sulfation of the fucose residues in fucoidans makes α-(1→3)-L-fucoside bonds inaccessible for the action of the studied enzymes. The maximum degree of cleavage of fucoidan was achieved by the fucoidanase from the marine bacterium Pseudoalteromonas citrea KMM 3296.

Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry

“…Although, such fragments were intensive in the spectra of the corresponding ions from the fucoidan of A. nodosum, 14 which is rich in the type of linkage mentioned above, and much less intense in the case of the fucoidan from F. evanescens, 15 which had about three times the prevalence of the (1?3)-over (1?4)-type of linkage. 23 MALDI-TOFMS of multisulfated fucooligosaccharides featured enhanced sensitivity over ESIMS, since singly charged ions of sodium salts were registered. Moreover, tandem MALDI-TOFMS spectra contained more intense fragment ions from the cross-ring cleavage of the 0,3 X-type and less intense of the 0,2 X-type.…”

Fucoidans from Brown Seaweeds Sargassum hornery, Eclonia cava, Costaria costata: Structural Characteristics and Anticancer Activity

“…The isolation and separation of water-soluble polysaccharides were carried out by modified methods [6,7]. Fresh or deep-frozen seaweed biomass (1 kg) was treated subsequently with ethanol, acetone, and chloroform.…”

Inhibition of thrombin and factor Xa by Fucus evanescens fucoidan and its modified analogs

“…146, No. 3, September, 2008 lysis of fucoidan was carried out as described previously [10]; the preparation was purified from polyphenols [4], from proteins by Sevag's method [1]; deacetylation was carried out by a previously described method [8]. Structural characteristics of fucoidan and its modified analogs are presented in Table 1.…”