A comparative study of the succinic dehydrogenase-cytochrome system in heart muscle and in kidney

Slater, E.C.

doi:10.1042/bj0450001

Cited by 212 publications

(4 citation statements)

References 12 publications

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“…The succinic dehydrogenase obtained here has many biochemical properties similar to the particulate enzyme from pig heart (Slater, 1949a). No additional cofactors are required for the reduction of 2,6-dichlorophenol indophenol or cytochrome c with the dialyzed enzyme.…”

Section: Discussionmentioning

confidence: 89%

The Succinic Dehydrogenase From Rhodospirillum Rubrum

Woody

Lindstrom

1955

J Bacteriol

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Succinic dehydrogenase has usually been associated with a macromolecular intracellular unit (mitochondria) whenever the enzyme has been isolated. In an ultracentrifugal analysis of the composition of extracts of various organisms, Schachman et al. (1952) found that Rhodospirillum rubrum yielded particles with a wide range of sedimentation constants. Succinic dehydrogenase has been demonstrated in extracts of R. rubrum by Vernon and Kamen (1953) using the reduction of cytochrome c or a dye as the assay, and by Eisenberg (1953) with a manometric assay. This article describes the properties of a partially purified succinic dehydrogenase system from R. rubrum that is associated with particles that have the same sedimentation constants as do the particles bearing pigments. A soluble bacterial succinic dehydrogenase has been obtained from Azotobacter vinelandii (Repaske, 1954). MATERIALS AND METHODS Rhodospirillum rubrum strain S-1 was maintained as stab cultures in this medium: yeast extract, 0.2 per cent; peptone, 0.2 per cent; and agar, 2 per cent in distilled water. Mass cultures were grown in 500 ml glass-stoppered reagent bottles under constant incandescent illumination for 5 days at 25 C in the following medium: sodium lactate, 0.5 per cent; yeast extract, 0.5 per cent; peptone, 0.5 per cent; K2HPO4, 0.1 per cent; potassium citrate, 0.04 per cent; MgC12, 0.02 per cent; CaCl2, 0.002 per cent in distilled water adjusted to pH 7.2 with KOH. The cultures were harvested by centrifugation, washed twice, and stored at-20 C until used. Equal weights of alumina and cells were ground 1 Authorized for publication as Paper No. 1907 in the journal series of the Pennsylvania Agricultural Experiment Station on September 21, 1954.

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Section: Discussionmentioning

confidence: 89%

The Succinic Dehydrogenase From Rhodospirillum Rubrum

Woody

Lindstrom

1955

J Bacteriol

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“…The succinic dehydrogenase (SDH) (EC 1.3.99.1) activity of the extracts was estimated manometrically, depending on the measurement of 02 consumption during succinate oxidation in the presence of potassium cyanide and methylene blue (22). The reac tion mixture was taken in the central well of the Warburg flask and contained 0.2 ml enzyme extract, 2.3 ml 36 mmol/1 phosphate buffer, pH 7.2, 0.3 ml 10 mmol/1 methylene blue and 0.3 ml 0.1 mol/1 KCN (neutralized).…”

Section: Methodsmentioning

confidence: 99%

Increased Histaminase Activity in the Atrophie Muscle of Denervated Frog

Rv¹

1977

Enzyme

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Sciatic denervation for 1 month in the frog Ram hexadactyla resulted in progressive atrophy of the gastrocnemius muscle without any change in the total DNA content of the whole muscle. Histamine content of the muscle decreased; glutamic acid content increased and histidine level remained unaltered on denervation. Histaminase activity localized in the muscle mitochondria increased on denervation. The histidine-degrading enzymes, histidine ammonia lyase, urocanate hydratase and imidazol-5yl lactate dehydrogenase, are localized in the sarcoplasm of the muscle and their activities are not altered on denervation. The histidine decarboxylase activity localized in the mitochondria is not altered on denervation. The reduction in the histamine content of the atrophied muscle may be due to increased mitochondrial histaminase activity but not due to increased decarboxylation of histidine. The loss of ‘trophic influence’ due to denervation may be manifested in the impairment of mitochondrial histaminase activity.

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“…The activity of succinic dehydrogenese was determined manometrically (Bonner 1955). It has been shown by Slater (1949) that no simple method for estimating succinic dehydrogenase has been found, hence, any assay for this enzyme has to be carried out in the presence of some or all the components of the succinic dehydrogenase-…”

Section: Succinic Dehydrogenasementioning

confidence: 99%

Studies on the mode of action of insecticides

Pant

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A comparative study of the succinic dehydrogenase-cytochrome system in heart muscle and in kidney

Cited by 212 publications

References 12 publications

The Succinic Dehydrogenase From Rhodospirillum Rubrum

The Succinic Dehydrogenase From Rhodospirillum Rubrum

Increased Histaminase Activity in the Atrophie Muscle of Denervated Frog

Studies on the mode of action of insecticides

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