A comparative study of the separation of oleanolic acid and ursolic acid in Prunella vulgaris by high-performance liquid chromatography and cyclodextrin-modified micellar electrokinetic chromatography

Abstract

A high-performance liquid chromatographic (HPLC) method and a cyclodextrin-modified micellar electrokinetic chromatographic (CD-MEKC) method were developed to separate and determine oleanolic acid (OA) and ursolic acid (UA) in Prunella vulgaris. HPLC separations were carried out on a Hedera ODS C 18 column with methanol -H 2 O-acetic acid (85:15:0.3, v/v/v) as mobile phase at a flow-rate of 0.8 ml min -1 . CD-MEKC analysis was performed on a CL1030 capillary electrophoresis system with a 6% (v/v) methanol solution (pH = 9.0) containing 10 mM disodium tetraborate, 10 mM sodium dihydrogen phosphate, 50 mM sodium dodecylsulfate (SDS), 15 mM 2-hydroxypropyl-ȕ-cyclodextrin (2-HP-ȕ-CD) as background electrolyte. The analytical results of HPLC and CD-MEKC were compared with each other. CD-MEKC has better analytical efficiency for two components, and the analytical time (15 min) was shorter than that of HPLC (35 min).

Preparation, characterization and evaluation of the in vivo trypanocidal activity of ursolic acid-loaded solid dispersion with poloxamer 407 and sodium caprate

“…Because the drug is a weak acid (pKa 5.29) (Du, Chen, 2009), its dissolution is favored in the pH 6.8 phosphate buffer medium, in which the more soluble ursolic acid's ionic form is predominant. Therefore, it is expected that drug will be preferably dissolved in the intestinal lumen (pH 6.8).…”

Application of Response Surface Methodology for Optimisation of Simultaneous UHPLC-PDA Determination of Oleanolic and Ursolic Acids and Standardisation of Ericaceae Medicinal Plants

“…There are several different approaches reported recently for quantification of OA and UA in plant materials including thin-layer chromatography [9], gas chromatography [10], capillary electrophoresis [11], high-performance liquid chromatography (HPLC), supercritical fluid chromatography [12], micellar electrokinetic chromatography [13], and nuclear magnetic resonance [14]. HPLC is, by far, the most frequently used technique in standardisation studies, mainly because of the high flexibility and relatively good ratio between the analytical performance and maintenance costs.…”

Cellular antioxidant and antiproliferative activity of ethanolic extract of Coleus tuberosus

“…Separation were carried out at 30°C on Eurosphere 100-5 C-18 column (5 micron, 250  4, 6 mm). The HPLC assay for quantitative determination of ursolic and oleanolic acids in EEPC and EEFC was carried out as described by Du and Chen (2009). Elution was performed at room temperature (23°C), humidity 55% and utilized the mobile phase consisted of a mixture, methanol (MeOH): 0.15% CH3COOH (90:10), monitored by wavelength 210 nm, and the flow rate was 1 ml/min.…”