A comparative study of the effects of cholesterol and desmosterol on zwitterionic DPPC model membranes

Altunayar, Cisem; Şahin, İpek; Kazancı, Nadide

doi:10.1016/j.chemphyslip.2015.03.006

Cited by 17 publications

(20 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thickness of the stacked bicelles was ≈40 nm as obtained from AFM measurements (Figure g,h), which implied their eight lipid bilayers stacked structure. The differential scanning calorimetry (DSC) thermogram of the stacked bicelles demonstrated a phase transition temperature of 40.3 °C (Figure S3, Supporting Information), slightly lower than reported value (41 °C) of liposomes in water, which indicated that ethanol molecules incorporated into stacked bicelles and influenced their phase transition temperature.…”

mentioning

confidence: 75%

Self‐Assembled “Breathing” Grana‐Like Cisternae Stacks

Han

2018

Advanced Materials

View full text Add to dashboard Cite

Membranes in cells display elaborate, dynamic morphologies intimately tied to defined cellular functions. Cisternae stacks are a common membrane morphology in cells widely found in organelles. However, compared with the well-studied spherical cell membrane mimics, cisternae stacks as organelle membrane mimics are greatly neglected because of the difficulty of fabricating this unique structure. Herein, the grana-like cisternae stacks are assembled via the reorganization of stacked microsized bicelles to mimic grana functions. The cisternae stacks are connected by fusion regions between adjacent cisternae. The number of cisternae can be controlled from ≈4 to 15 by the variation of ethanol volume percentage. Under the stimulation of solvent or negatively charged nanoparticles, the cisternae stacks can reversibly compress and expand, similar to the "breathing" property of natural grana. During the "breathing" process, nanoparticles are reversibly captured and released. Frequency resonance energy transfer is realized on the cisternae stacks trapped with two kinds of quantum dots. The cisternae stacks provide advanced membrane model for cell biotechnology, and clues for the shaping of organelles composed of cisternae. The ability of the cisternae stacks to capture materials enables them to possibly be applied in biomimetics and the design of advanced functional materials.

show abstract

mentioning

confidence: 75%

Self‐Assembled “Breathing” Grana‐Like Cisternae Stacks

Han

2018

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…Displaying a very high complexity with varied lipid, proteins and carbohydrates, biological membranes are accompanied by some disadvantages to examine drug-lipid interactions because of difficulties in the interpretation of the results. Thus, usage of model membrane systems which offer specific information is a general approach for such studies 18,[22][23][24] . Specifically, the composition of outer leaflet of plasma membranes of mammalian cells consists of phosphatidylcholines (PC) and sphingomyelin, which together reach to abundant amounts in most membranes, and the other main components such as cholesterol (CHO) found up to 50 mol %23,25.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, the composition of outer leaflet of plasma membranes of mammalian cells consists of phosphatidylcholines (PC) and sphingomyelin, which together reach to abundant amounts in most membranes, and the other main components such as cholesterol (CHO) found up to 50 mol %23,25. Model systems made up of CHO and major lipid components such as PC lipids like dipalmitoylphoshatidiylcholine (DPPC) have been widely used to mimic biological membranes [13][14][15]21,22,24,25 .…”

Section: Introductionmentioning

confidence: 99%

“…By monitoring such changes in model membrane systems the information about the degree of interaction and behaviour of the drug in real membrane systems can be obtained 14,17,[26][27] . Similarly, in the present study we aimed to provide information for binding ability of LCM with PC membrane lipids, and designed our experiments on a well-established molecular models of biomembranes 13,18,22,24,26 , multilamellar vesicles (MLVs) formed by DPPC and CHO lipids, as similarly performed for phenytoin, 12 and gabapentin and levetricetam 13 . Indeed, in the absence and the presence of CHO (10 mol%), the effects of LCM at different concentrations (1-10-20 mol%) on biophysical parameters of DPPC MLVs were investigated.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, in the absence and the presence of CHO (10 mol%), the effects of LCM at different concentrations (1-10-20 mol%) on biophysical parameters of DPPC MLVs were investigated. To achieve this, Fourier transform infrared (FT-IR) spectroscopy and differential scanning calorimetry (DSC) were utilized as in earlier studies 12,13,[17][18][19]22,[24][25][26][27] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Binding Ability of Lacosamide to Phosphatidylcholine Lipids and Cholesterol Effects

Yağış¹,

Kaya²

2018

MMJ

View full text Add to dashboard Cite

Aim: In the present study, in order to obtain information about binding profile of lacosamide (LCM) with membrane lipids, which is the most important parametre that determines its availability in the central nervous system, we aimed to analyze molecular interaction of LCM with model membranes formed by well-known representative lipids of biological membranes. Methods: The effects of varied concentrations (1-10-20 mol %) of LCM on biophysical parameters of dipalmitoylphosphatidylcholine multilammelar vesicles in the absence and presence of cholesterol (10 mol %) were studied by differential scanning calorimetry and fourier transform infrared spectroscopy. Results: According to the data obtained in all concentrations LCM decreased main transition temperature and enthalpy with broaden transition curve. However, LCM at 1 mole % affected such values mostly in comparison with 10-20 mol% concentrations. With the addition of CHO to 20 mol % of LCM sample, these parameters were significantly lowered. Moreover, LCM at high concentrations prominently increased both order of lipid phases, and hydrogen binding capacity of glycerol and phosphate groups of lipids but reduced fluidity. Also, CHO was found to tend to reverse these effects of LCM except for order and fluidity parameters. Conclusion: According to the findings obtained, in accordance with its chemical structure, LCM leads to alteration of thermotrophic parameters, structures and functions of phosphatidylcholine lipids abundant in brain cell membrane, which also show the penetration ability of LCM into biological membranes. But, the degree of such behavior can be dependent on applied concentration of LCM and presence of CHO. In summary, all data may provide information for its use in therapeutic strategies of epilepsy and development of new antiepileptics.

show abstract

Liposome-Permeability Templating of Gadolinium Hydroxide Nanostructures

Municoy

Bellino

2016

ChemistrySelect

View full text Add to dashboard Cite

With the growing attention in building novel nanostructures, there is a need for general approaches to controlling the sizes and shapes in precipitation processes. Here, precipitated gadolinium hydroxides (Gd(OH) 3 ) were made in the form of hollow spheres by control of the liposome permeability. Addition of Gd(NO 3 ) 3 to the acidic solution containing the liposomes entrapping OH À creates a self-precipitating template in the precipitation process. The control-formation of these nano-structures takes place through a liposome-membrane phase change. Hybrid nanocapsules are obtained when entrapped hydroxyl ions (OH À ) permeates from the liposome and shell material precipitates by forming Gd(OH) 3 . The morphology of the final product is molded on the original liposome. This synthesis strategy might have implications in significant applications, such as drug delivery systems, and in mineralization, where many processes are also membrane-assisted.

show abstract

A comparative study of the effects of cholesterol and desmosterol on zwitterionic DPPC model membranes

Cited by 17 publications

References 32 publications

Self‐Assembled “Breathing” Grana‐Like Cisternae Stacks

Self‐Assembled “Breathing” Grana‐Like Cisternae Stacks

Binding Ability of Lacosamide to Phosphatidylcholine Lipids and Cholesterol Effects

Liposome-Permeability Templating of Gadolinium Hydroxide Nanostructures

Contact Info

Product

Resources

About