A comparative study of whole-blood platelet aggregation in laboratory animals: its species differences and comparison with turbidimetric method

Kurata, Masahiro; Ishizuka, Nobuhiko; Matsuzawa, Mizuho; Haruta, Koichi; Takeda, Kazuki

doi:10.1016/0742-8413(95)02032-2

Cited by 19 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These variations are particularly apparent in the turbidometric aggregation model (Born 1962) using canine PRP (Chignard & Varagaftig 1976). Aggregation to AA in canine whole blood is usually less variable because of potentiation of the platelet response to AA by other mediators not present in PRP (Grauer et al 1992;Kurata et al 1995) but highly variable responses to AA have been reported (Jüttner et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Single oral dose study of two isosorbide-based aspirin prodrugs in the dog

Gilmer

Murphy

Shannon

et al. 2003

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

The objective of this study was to compare two aspirin prodrugs, isosorbide diaspirinate (ISDA) and a nitroaspirin (ISMNA), with aspirin in terms of effects on dog platelet function after administration of a single oral dose. Groups of six dogs were administered ISDA (2 mg kg -1 ), ISMNA (4 mg kg -1 ) or aspirin (2 mg kg -1 ). Blood was sampled at 1, 2, 4, 8, 12 and 24 h postdosing and evaluated for capacity to generate post-clotting thromboxane (TX)B 2 . The aggregation response to arachidonic acid (AA) (100 M), ADP (30 M) or collagen (10 g mL -1 ) was estimated at each time-point using the whole blood impedance method. Plasma ISMN following oral administration of ISMNA was also measured and compared with plasma ISMN following administration of a physical mixture of ISMN and aspirin. ISDA administration (2 mg kg -1 ) was associated with a significant reduction (P < 0.05) in serum TXB 2 at 12 and 24 h (>90%) post-dosing and persistent inhibition of AAinduced platelet aggregation. ISDA administration caused a more marked depression of post-clotting TXB 2 levels than aspirin in this study, although its ability to inhibit platelet aggregation was less consistent than that of aspirin. The nitroaspirin ISMNA was least effective at inhibiting platelet aggregation response or TXB 2 production. The ISMN AUC 0-24 h for the ISMNA-treated dogs was 77% of that for the physical mix-treated dogs and the t max was delayed. This study indicates that the two aspirin esters cause aspirin-like effects on platelet function, probably through aspirin release, when administered orally to dogs.

show abstract

Section: Discussionmentioning

confidence: 99%

Single oral dose study of two isosorbide-based aspirin prodrugs in the dog

Gilmer

Murphy

Shannon

et al. 2003

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

show abstract

“…Interestingly, previous researchers showed that dogs responded satisfactorily to ADP in 3 doses (5, 10, and 20 μM), which might be explained by the fact that they studied aggregatory response in whole blood 23. Whether the presence of erythrocytes and leukocytes can heighten platelet sensitivity against collagen and ADP is an issue yet to be determined, although one report has shown this to be a possibility 24. Epinephrine may enhance aggregation because of its synergistic action over other agonists, by acting in a different aggregatory pathway such as the α 2 adrenoreceptor pathway 8.…”

Section: Discussionmentioning

confidence: 99%

Platelet aggregation studies in acute experimental canine ehrlichiosis

Brandão¹,

Hasegawa²,

Hagiwara³

et al. 2006

Veterinary Clinical Pathol

View full text Add to dashboard Cite

show abstract

“…With the whole-blood platelet aggregation method, (1) sample reparation is easy, and (2) the detection of platelet inhibition via adenosine-uptake inhibition in erythrocytes, such as a group of dipyridamole, is possible (Dawicki et al, 1985;Kurata et al, 1997). However, one of the disadvantages is its low sensitivity to adenosine 5'-diphosphate (ADP)-or platelet activating factor (PAF)-induced platelet aggregation in rats, dogs and rabbits (Kurata et al, 1995).…”

Section: Impedance Methodsmentioning

confidence: 99%

“…On the other hand, there exist large species differences regarding platelet aggregation (Macmillan and Sim, 1970;Dodds, 1978;Kurata et al, 1995), coagulation factors and fibrinolysis (Karges et al, 1994;Ravanat et al, 1995;Dodds, 1997). In addition, methodological limitations in each species are an essential issue that complicates the examinations done in laboratory animals.…”

Section: Introductionmentioning

confidence: 99%

Blood Coagulation Tests in Toxicological Studies-Review of Methods and Their Significance for Drug Safety Assessment-

Kurata

Horii²

2004

J. Toxicol. Sci.

Self Cite

View full text Add to dashboard Cite

In general toxicological studies, prothrombin time and activated partial thromboplastin time are routinely measured to assess blood coagulation. Special (problem-driven) tests for blood coagulation are of significance to detect abnormalities and investigate the mechanism of toxicity in detail. In this review, we compiled widely scattered information on blood coagulation testing from different fields in the biological area, and reviewed the methods available and their significance in toxicological studies. The relevant literature cited here reports large species differences in platelet aggregation, coagulation factors or fibrinolysis, and technical limitations. However, the following tests are basically applicable to laboratory animals; (1) assays for individual coagulation factors and protein induced by vitamin K absence or antagonists (PIVKA) to investigate coagulation factor abnormalities; (2) platelet aggregation-, platelet adhesion-, platelet release-tests and von Willebrand factor assay to screen and/or investigate platelet dysfunction; (3) fibrin/fibrinogen degradation products (FDP), D-dimer and thromboelastogram to detect fibrinolitic abnormalities, and assays for plasminogen, plasmin and their activator/inhibitor to investigate fibrinolysis in detail; and (4) bleeding-time to grossly evaluate blood coagulation capability in vivo. An appropriate battery of these tests provides significant information for risk assessment of drugs.

show abstract

A comparative study of whole-blood platelet aggregation in laboratory animals: its species differences and comparison with turbidimetric method

Cited by 19 publications

References 22 publications

Single oral dose study of two isosorbide-based aspirin prodrugs in the dog

Single oral dose study of two isosorbide-based aspirin prodrugs in the dog

Platelet aggregation studies in acute experimental canine ehrlichiosis

Blood Coagulation Tests in Toxicological Studies-Review of Methods and Their Significance for Drug Safety Assessment-

Contact Info

Product

Resources

About