Platelet aggregation studies in acute experimental canine ehrlichiosis

Brandão, Leonardo Pinto; Hasegawa, M. Y.; Hagiwara, Mitika K.; Kohayagawa, Aguemi

doi:10.1111/j.1939-165x.2006.tb00091.x

Cited by 11 publications

(14 citation statements)

References 16 publications

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“…Conditions like hepatomegaly and splenomegaly [2] as well as lymphadenomegaly [22] were detected mostly in acute conditions. Bleeding tendencies may be attributed to a combination of mild thrombocytopenia and vasculitis [28]. Out of the 70 positive cases, six dogs succumbed to death (four pure CME, one with additional infection of B. gibsoni and other with T. evansi), 50 % of which were German Shepherd echoing the more severe form with poor prognosis in this breed [2].…”

Section: Clinico-incidental Studymentioning

confidence: 99%

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Behera¹,

Dimri

Banerjee

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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The present study was aimed at detecting natural cases of canine monocytic ehrlichiosis (CME) by molecular and cytological methods and assessment of clinico-patho-biochemical parameters among the affected population. Nested polymerase chain reaction was found to be highly sensitive (47.7 %) in detecting the acute cases, followed by buffy coat (29.5 %) and blood smear examination (22.7 %). CME incidence rate was found to be 8.4 %. Hemogram revealed significant (P \ 0.01) depletion in the levels of hemoglobin, hematocrit, total erythrocyte count, monocyte and platelet count in diseased dogs as compared to healthy controls. Plasma biochemistry revealed hypoproteinemia, hypoalbuminemia and hyperglobulinemia along with significantly (P \ 0.05) higher blood urea nitrogen and alanine aminotransferase values. CME revealed non-significant increase in the levels of erythrocytic lipid peroxides, reduced glutathione and activity of superoxide dismutase, whereas activities of glutathione-S-transferase and glutathione reductase showed significant (P \ 0.05) increase and decrease, respectively as compared to healthy controls. Plasma levels of cortisol, insulin and blood level of copper showed non-significant (P [ 0.05) reduction, whereas blood level of zinc showed significant (P \ 0.05) reduction as compared to healthy controls. These findings revealed that changes in erythrocytic oxidant-antioxidant profile and blood mineral concentration in canine monocytic ehrlichiosis are largely nonsignificant and inconsistent. Hence, further study is required to elucidate their role in pathogenesis of canine monocytic ehrlichiosis.

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Section: Clinico-incidental Studymentioning

confidence: 99%

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Behera¹,

Dimri

Banerjee

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…Thus, it has been suggested that bleeding in ehrlichiosis is of multifactorial origin 25. In consequence, there are tangible explanations and support as to why our patient developed cerebral haemorrhage without thrombocytopaenia.…”

Section: Discussionmentioning

confidence: 56%

Cerebral haemorrhage as a clinical manifestation of human ehrlichiosis

García-Baena

Cárdenas

Ramón³

2017

BMJ Case Reports

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A 16-year-old young man presented to the emergency room with new-onset generalised tonic-clonic seizures. Examination showed a Glasgow score of 13 and predominantly crural left hemiparesis. Imaging demonstrated a right frontoparietal haemorrhage of non-vascular origin with perilesional oedema. Surgical drainage was carried out, but rebleeding occurred within 24 hours following surgery, and again 1 week after discharge. On reinterrogation and examination, infection was suspected and empirical management with doxycycline was begun. Improvement was evident 72 hours after antibiotic initiation, and PCR confirmed the diagnosis; thus, doxycycline was continued for 6 months. After 2 years, seizures recurred and treatment was reinstated with good clinical response. However, seizures reappeared whenever treatment discontinuation was attempted. Lacking alternatives, doxycycline was maintained up to the third year following the initial episode. Subsequently, the patient showed complete resolution without neurological sequelae up to his last follow-up visit, 12 months following treatment cessation.

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“…Estudos anteriores apontam apenas alterações na função plaquetária de cães com erliquiose (Gaunt et al 1990, Harrus et al 1996, Brandão et al 2006, Cortese et al 2006, sendo este o primeiro relato de alterações hemostáticas em cães com erliquiose sem envolvimento imuno-mediado.…”

Section: Discussionunclassified

Avaliação das alterações hemostáticas e do risco tromboembólico em cães com AHIM

et al. 2016

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ABSTRACT.-Moraes L.F., Takahira Immune-mediated hemolytic anemia (IMHA) is the most common cause of hemolytic anemia and the most prevalent immune-mediated disease in dogs, which is classified in primary or secondary. IMHA has been associated with hypercoagulability state and thromboembolism has been referred as the most common complication. The purpose of this study was to correlate the possibility of hemostatic abnormalities and the thromboembolic risk in IMHA and in anemias of other etiologies. 76 dogs were selected, all exhibiting hematocrit lower than 20% and at least one clinical sign commonly associated with IMHA or breed predisposition. These dogs were tested for IMHA by flow cytometry. Hemostatic tests (platelet count, PT, APTT, TT, AT, PDF and D-dimer) as well as CBC, reticulocyte count, blood parasite search in peripheral blood smear, Ehrlichia sp. and leptospirosis tests were performed in all dogs. 59 dogs were positive for IMHA. The thromboembolic risk was characterized by the presence of three or more changes in the tests of the hemostatic profile. 74.6% cases of IMHA were attributed to infectious diseases and was mostly associated with Ehrlichia sp. (88.6%). 72.1% of dogs had thrombocytopenia and 57.6% had regenerative anemia with significantly higher values of metarubricyte and reticulocyte count. There were no significant changes between two anemic groups (positive and negative for IMHA). Anemic dogs had higher APTT mean value and lower AT and platelet counts mean values than the control group (p<0.05). 25 dogs with IMHA and seven dogs without IHMA had thromboembolic state. The specificity of PDF was lower (30.2%) than previous studies. The choice of the Ig class does not affect the diagnosis of IMHA. There were no correlation between the presence of antibodies bound to the RBC surface and the hemostatic tests, but there were a week correlation (p<0,05) between Ht(%) and APTT (r=-0.2621), AT (r=0.4297) and platelets count (r=0.5349) values. Anemia is associated with hemostatic abnormalities and thromboembolic risk, however the immune-mediated nature of the disease was not the determining factor for this condition. In part, these changes can be attributed to infection diseases seen in both groups (positive and negative for IMHA). The similar thromboembolic risk evidenced in both groups of anemic dogs does not exclude the relationship of immune-mediated involvement, but alert to the possibility of thromboembolism in anemic dogs with Ehrlichia sp. (negative for IHMA). Therefore, anemic dogs with clinical signs associated with thromboembolic risk should be evaluated for this complication, today poorly 1 Recebido em 6 de junho de 2015.Aceito para publicação em 8 de março de 2016.

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Platelet aggregation studies in acute experimental canine ehrlichiosis

Abstract: The tendency of dogs infected with E canis to bleed may be related not only to thrombocytopenia but also to platelet dysfunction associated with the disease.

Cited by 11 publications

References 16 publications

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India

Cerebral haemorrhage as a clinical manifestation of human ehrlichiosis

Avaliação das alterações hemostáticas e do risco tromboembólico em cães com AHIM

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