Scale, comfort & safety (t=2.876, p=.005), feeding (t=2.281, p=.024), infant's behavior (t=2.395, p=.018), hygiene control (t=2.978, p=.003), p<.001) and confidence in newborn care showed significant total scores (t=2.997, p=.003). Based on the Fatigue Continuum Form, the physical, psychological, and neurosensory fatigue scores and the total fatigue score did not show any statistical significance in each area (t=1.169, p=..244)(t=1.941, p=.054)(t=1.863, p=.064)(t=1.897, p=.060). The present findings can be applied as the reference data conducive to correcting the myth among puerperal mothers that rooming-in will increase the postnatal fatigue and supporting the benefits of rooming-in.