A comparative study on the phosphoglyceric acid cycle in mammalian erythrocytes

The Phosphoglycerate Mutases

“…Inactivation of rabbit muscle phosphoglycerate mutase with the sulfydryl reagent ''C-N-bromoacetylethanolamine phosphate enabled a labeled tryptic peptide to be isolated (Hartman and Norton, 1976). This peptide was shown to have the composition Trp, Lys, Cys, Asx, Ser, Glx2, Gly, Ala, Leu, Phe2, which corresponds perfectly with the sequence of residues 19-30 in the recently published sequence of the human muscle enzyme (Shanske et al, 1987).…”

Red Cell Metabolism in High and Low Glutathione Goats

“…These results confirm the observa- tion that when compared to other mammalian species tested so far, ruminants have strikingly lower concentrations of 2,3-DPG [Harkness et al, 1969;Battaglia et al, 1970;Agar and Smith, 1973]. It has been shown by Perutz [1970], and Bunn [1971] that the ß-chain of sheep and goat haemo globins lack the amino terminal residue which participates in the binding of 2,3-DPG.…”

The first case of a complete deficiency of diphosphoglycerate mutase in human erythrocytes.

“…This enzyme presents a single electrophoretic band (6) and represents one of the three molecular forms of erythrocyte MPGM (8). Species differences of erythrocyte DPGM have been described in the erythrocytes of mammals, such as the cat, and also in ruminants whose cells contain very low levels of 2,3-DPG (11). Thus the activity of this enzyme in vivo is probably an important determinant of2,3-DPG levels within the erythrocytes.…”