A comparative study on UV spectrophotometric quantification of DNA extracted from human saliva

Saroch, Gaurav; Paul, Monit

doi:10.1016/j.ejfs.2012.08.003

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Nucleic acid leakage was quantified at 260 nm using a spectrophotometer [ 31 ]. The formula for the quantity of DNA was as follows [ 32 ]:

…”

Section: Methodsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity

Yang,

Guo,

Long

et al. 2023

Nanomaterials

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The synthesis of metal nanomaterials is a timely topic due to their widespread use in fields such as crop protection, the environment, medicine, and engineering. Green synthesis of nanoparticles, which uses plant extracts instead of industrial chemical agents to reduce metal ions, has been developed to decrease costs, reduce pollution, and improve environmental and human health safety. In this paper, silver nanoparticles (AgNPs) were synthesized from the flower extract of Jasminum nudiflorum. The green synthesized AgNPs were characterized by UV-Vis, FTIR, XRD, SEM, and other technologies. The antifungal activity of the prepared AgNPs against Alternaria longipes was tested using the plate method, the concentration dilution method, and other methods, and the antioxidant activity of the prepared AgNPs was evaluated by DPPH and hydroxyl free scavenging methods. The results showed that AgNPs synthesized from J. nudiflorum flower extract have a face-centered cubic structure (fcc), and the average grain size of the nanoparticles is 13 nm; they are also mainly spherical in shape. Additionally, the concentration of AgNPs (ranging from 16 to 128 μg/mL) significantly inhibited the mycelial growth of A. longipes in comparison to the control. The inhibitory rate gradually increased with increasing AgNP concentration, ranging from 70.64% to 79.60% at a concentration of 128 μg/mL. The minimum inhibitory concentration was observed at 32 μg/mL. AgNPs induced overaccumulation of MDA in A. longipes, resulting in cell membrane damage and nucleic acid leakage. Moreover, the AgNPs have significant antioxidant properties, which increase with increasing concentration. The clearance rate of DPPH was 25.46 ± 0.90% when the concentration of AgNPs was 8 μg/mL, and the clearance rate of the hydroxyl radical was 28.62 ± 0.59% when the concentration of AgNPs was 128 μg/mL. Thus, the flower extract from J. nudiflorum holds potential as an environmentally friendly and green alternative for the synthesis of AgNPs, which have antifungal and antioxidant potential.

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“…Nucleic acid leakage was quantified at 260 nm using a spectrophotometer [ 31 ]. The formula for the quantity of DNA was as follows [ 32 ]:

…”

Section: Methodsmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity

Yang,

Guo,

Long

et al. 2023

Nanomaterials

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“…There are various optical methods to analyze specific content in saliva. UV spectrophotometry is used to detect DNA in saliva [3], chemiluminescence is employed to monitor continuously roxithromycin levels in saliva via flow injection method [4], spectral analysis of saliva to detect lung cancer marker uses the surface enhanced Raman spectroscopy method [5], infrared attenuated total reflection spectroscopy is utilized to detect drugs by saliva spectral analysis [6] and testosterone levels are measured in patients with androgenic disorders using the saliva luminescence immunoassay [7]. Each and every method measures different components of saliva and have their own unique range of spectrum which differs from each other.…”

Section: 10 Introductionmentioning

confidence: 99%

Development of Spectral Delayed Luminescence System for Whole Saliva Analysis: A Prototype Study

Malarvili

2014

Jurnal Teknologi

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This paperwork presents the development of spectral delayed luminescence system for the measurement of whole saliva. Despite various reports of delayed luminescence and its correlation to various diseases, it has fail to highlight the specific cause of the occurrence suggesting it being more suitable as a screening tool utilizing saliva as non-invasive sampling medium. Therefore it is the aim of this paper to have the system critical performance tested before putting it to use at the field. The system prototype is tested for its response during room temperature operation and during spot cooling of the photomultiplier tube module. Delayed luminescence of saliva is compared over distilled water as a control. The spectral delayed luminescence of saliva is compared to that of the dimmed white light for functional testing purposes. Through experiments, the best operation for the system is at constant room temperature with light stimulation time determined at 150 ms. Being 98% water, the delayed luminescence of saliva is slightly greater than that of distilled water. Given light stimulation of wide spectrum from white LED, the delayed luminescence of saliva is accumulated between 495 nm and 530 nm. Results demonstrated that the system for spectral delayed luminescence of saliva is viable and capable for acquiring data for further studies.

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A comparative study on UV spectrophotometric quantification of DNA extracted from human saliva

Cited by 2 publications

References 5 publications

Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity

Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity

Development of Spectral Delayed Luminescence System for Whole Saliva Analysis: A Prototype Study

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