A comparative transcriptome and proteomics study of post‐partum ovarian cycle arrest in yaks (Bos grunniens)

Abstract

Post‐partum ovarian cycle arrest is the main factor affecting yak reproductive efficiency. There are few reports regarding the molecular regulatory mechanism of post‐partum oestrus at transcriptome and proteome levels in yaks. Our previous studies focussed on the ovaries of yaks with post‐partum ovarian cycle arrest and post‐partum oestrus yaks. In this study, RNA sequencing transcriptomic study was combined with quantitative proteomic analyses to identify post‐partum ovarian cycle‐related genes and proteins. Consequently, 1,149 genes and 24 proteins were found to be up‐ or downregulated during post‐partum oestrus. The analysis of differentially regulated genes identified three gene or protein pairs that were synchronously upregulated and no gene or protein pairs that were synchronously downregulated, suggesting that these upregulated genes may regulate the post‐partum ovarian cycle. The functional classification of these differentially expressed genes and proteins indicated their connection with the oocyte meiosis, the oestrogen signalling pathway, the progesterone‐mediated oocyte maturation and the gonadotrophin‐releasing hormone (GnRH) signalling pathway. In this study, a total of six genes and two proteins involved in the oocyte meiosis, the oestrogen signalling pathway, the progesterone‐mediated oocyte maturation and the GnRH signalling pathway were identified. The CSNK1A1, M91_09723, M91_11326, M91_21439, M91_19073, SHC2, Atf6b, M91_03062, HSPCA and calmodulin could regulate oestrus, respectively, in the post‐partum so as to control the anoestrus status.

Study of Transcriptomic Analysis of Yak (Bos grunniens) and Cattle (Bos taurus) Pulmonary Artery Smooth Muscle Cells under Oxygen Concentration Gradients and Differences in Their Lung Histology and Expression of Pyruvate Dehydrogenase Kinase 1-Related Factors

“…The sequencing of the yak genome was first published in 2012 [26], which laid an important foundation for subsequent yak−related studies. Among the previous studies on hypoxia−adapted transcription in yaks, most of the previous studies on the transcriptome of yaks (Bos grunniens) focused on reproduction and nutrition [27][28][29][30]. The studies on the lung transcriptome of high−altitude yaks and low−altitude cattle (Bos taurus) focused on exploring the potential mechanisms of yak adaptation to high−altitude environments [25,26,31,32], but no studies have ever been conducted at the cellular level to reveal the mechanism of yaks' adaptation to hypoxia.…”

Proteomic quantification of native and ECM-enriched mouse ovaries reveals an age-dependent fibro-inflammatory signature

“…To our knowledge, this is the first study to utilize unbiased proteomic approaches to investigate the effect of reproductive aging on the murine ovarian proteome and matrisome. However, several other groups have utilized proteomic methods to characterize the composition of the ovary, as well as ovarian structures and cell types, including antral follicles, corpora lutea, and granulosa cells from various species [26,34,[50][51][52][53][54][55][56][57][58]. Studies that profiled fetal mouse ovaries, mouse ovaries in the context of the pubertal transition, or mouse ovarian follicles identified proteins implicated in steroidogenesis, the cell cycle, the complement cascade, ECM-receptor interaction, and metabolic regulation including glycolysis and the electron transport chain [56][57][58].…”