A Comparative Transcriptomic Analysis Reveals That HSP90AB1 Is Involved in the Immune and Inflammatory Responses to Porcine Deltacoronavirus Infection

Zhao, Yujia; Chen, Rui; Xiao, Dai; Zhang, Luwen; Song, Daili; Wen, Yiping; Wu, Rui; Zhao, Qin; Du, Senyan; Wen, Xintian; Cao, Sanjie; Huang, Xiaobo

doi:10.3390/ijms23063280

Cited by 8 publications

(4 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result was also confirmed by another NFκB inhibitor SC75741 ( Fig. S5B ) ( Reda et al, 2022 ; Zhao et al, 2022 ). Curiously, when treating cells with rapamycin (RAPA), a mTORC1-specific inhibitor, the activation of NFκB cannot be repressed, which may explain the unidirectional regulatory effect of NFκB on mTORC1 ( Figs.…”

Section: Resultssupporting

confidence: 66%

TLR7 promotes skin inflammation via activating NFκB-mTORC1 axis in rosacea

Huang,

Liu,

Chen

et al. 2023

PeerJ

View full text Add to dashboard Cite

Rosacea is a chronic inflammatory skin disease originated from damaged skin barrier and innate/adaptive immune dysregulation. Toll-like receptors (TLRs) sense injured skin and initiate downstream inflammatory and immune responses, whose role in rosacea is not fully understood. Here, via RNA-sequencing analysis, we found that the TLR signaling pathway is the top-ranked signaling pathway enriched in rosacea skin lesions, in which TLR7 is highlighted and positively correlated with the inflammation severity of disease. In LL37-induced rosacea-like mouse models, silencing TLR7 prevented the development of rosacea-like skin inflammation. Specifically, we demonstrated that overexpressing TLR7 in keratinocytes stimulates rapamycin-sensitive mTOR complex 1 (mTORC1) pathway via NFκB signaling. Ultimately, TLR7/NFκ B/mTORC1 axis promotes the production of cytokines and chemokines, leading to the migration of CD4+T cells, which are infiltrated in the lesional skin of rosacea. Our report reveals the crucial role of TLR7 in rosacea pathogenesis and indicatesa promising candidate for rosacea treatments.

show abstract

Section: Resultssupporting

confidence: 66%

TLR7 promotes skin inflammation via activating NFκB-mTORC1 axis in rosacea

Huang,

Liu,

Chen

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

“…Transcriptome analysis was used to quantitatively identify DEGs after PDCoV infection in ST cells. PDCoV infection activates the NF-κB signaling pathway and leads to the expression of inflammatory factors, which may be related to TLRs, but TLR2 is not a critical factor [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of LLC-PK Cells Single or Coinfected with Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus

Lu,

Yu,

Tong

et al. 2023

Viruses

View full text Add to dashboard Cite

Porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) are the two most prevalent swine enteric coronaviruses worldwide. They commonly cause natural coinfections, which worsen as the disease progresses and cause increased mortality in piglets. To better understand the transcriptomic changes after PEDV and PDCoV coinfection, we compared LLC porcine kidney (LLC-PK) cells infected with PEDV and/or PDCoV and evaluated the differential expression of genes by transcriptomic analysis and real-time qPCR. The antiviral efficacy of interferon-stimulated gene 20 (ISG20) against PDCoV and PEDV infections was also assessed. Differentially expressed genes (DEGs) were detected in PEDV-, PDCoV-, and PEDV + PDCoV-infected cells at 6, 12, and 24 h post-infection (hpi), and at 24 hpi, the number of DEGs was the highest. Furthermore, changes in the expression of interferons, which are mainly related to apoptosis and activation of the host innate immune pathway, were found in the PEDV and PDCoV infection and coinfection groups. Additionally, 43 ISGs, including GBP2, IRF1, ISG20, and IFIT2, were upregulated during PEDV or PDCoV infection. Furthermore, we found that ISG20 significantly inhibited PEDV and PDCoV infection in LLC-PK cells. The transcriptomic profiles of cells coinfected with PEDV and PDCoV were reported, providing reference data for understanding the host response to PEDV and PDCoV coinfection.

show abstract

“…Studies have shown that HSPA8 interacts with PRRSV GP4 through its PB domain and participates in the attachment and internalization of PRRSV [36]. HSP90AB1, a member of the HSP90 family, is involved in the immune and inflammatory responses to COVID-19 [37], ASFV [38], and PDCoV [39]. It is an attractive antiviral target.…”

Section: Discussionmentioning

confidence: 99%

Target Discovery of Matrine against PRRSV in Marc-145 Cells via Activity-Based Protein Profiling

Ling

Cao

Sun

et al. 2023

IJMS

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome (PRRS) seriously endangers the sustainable development of the pig industry. Our previous studies have shown that matrine can resist porcine reproductive and respiratory syndrome virus (PRRSV) infection. This study aimed to explore the anti-PRRSV targets of matrine in Marc-145 cells. Biotin-labeled matrine 1 and 2 were used as probes. MTT assay was used to determine the maximum non-cytotoxic concentration (MNTC) of each probe in Marc-145 cells. The anti-PRRSV activity of each probe was evaluated via MTT, qPCR and Western blot, and its anti-inflammatory activity was evaluated via qPCR and Western blot. The targets of matrine in Marc-145 cells were searched using activity-based protein profiling (ABPP), and compared with the targets predicted via network pharmacology for screening the potential targets of matrine against PRRSV. The protein–protein interaction networks (PPI) of potential targets were constructed using a network database and GO/KEGG enrichment analysis was performed. ACAT1, ALB, HMOX1, HSPA8, HSP90AB1, PARP1 and STAT1 were identified as potential targets of matrine, and their functions were related to antiviral capacity and immunity. Matrine may play an anti-PRRSV role by directly acting on ACAT1, ALB, HMOX1, HSPA8, HSP90AB1, PARP1 and STAT1.

show abstract

A Comparative Transcriptomic Analysis Reveals That HSP90AB1 Is Involved in the Immune and Inflammatory Responses to Porcine Deltacoronavirus Infection

Cited by 8 publications

References 66 publications

TLR7 promotes skin inflammation via activating NFκB-mTORC1 axis in rosacea

TLR7 promotes skin inflammation via activating NFκB-mTORC1 axis in rosacea

Transcriptome Analysis of LLC-PK Cells Single or Coinfected with Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus

Target Discovery of Matrine against PRRSV in Marc-145 Cells via Activity-Based Protein Profiling

Contact Info

Product

Resources

About