A comparison between polymerase chain reaction (PCR) and traditional techniques for the diagnosis of leptospirosis in bovines

Hernández-Rodríguez, Patricia; Díaz, César; Dalmau, Ernesto; Quintero, Gladys

doi:10.1016/j.mimet.2010.10.021

Cited by 54 publications

(57 citation statements)

References 18 publications

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“…Based on the results reported for Colombia and considering: 1) that more than 80% of the studies on the prevalence of the disease were based on the MAT, 2) the difficulties of interpreting the MAT and low titers, and 3) the doubtful results on the efficacy of diagnostic tests, including the MAT [11][12][13][14][15], it can be concluded that the real prevalence of the disease in the country is still unknown and that there is a need to continue to search for methods that can accurately assess its prevalence. Work at molecular level is revealing interesting results in various parts of the world and should be considered in future works [10][11][12][13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…Work at molecular level is revealing interesting results in various parts of the world and should be considered in future works [10][11][12][13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

“…Other methods include: dark field examination, immunofluorescence, culture, Elisa IgM, dot Elisa, and polymerase chain reaction (PCR). The literature on these methods is extensive, and several authors have discussed the advantages and limitations of each method, as well as the discrepancies between the diagnostic results [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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A retrospective analysis of the leptospirosis research in Colombia

Dechner

2014

J Infect Dev Ctries

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Introduction: Leptospirosis is a re-emerging infectious disease whose prevalence is often underestimated, not only in Colombia, but in most developing countries. The objective of this paper is to assess the research status of leptospirosis in Colombia in order to identify trends, knowledge gaps, and directions for future research. Methodology: With the aim of gathering all the information available on leptospirosis in the country, a web search was conducted in various indexes and databases. The search terms used were: Leptospirosis + Colombia. A total of 95 publications were found and analyzed using descriptive statistics. Results: The first report of leptospirosis in the country was published in 1957. More than 60% of the published works focus on the prevalence of the disease. The Central part of the country is the area with the highest number of publications. MAT was used in 84% of the prevalence studies and humans are the most studied taxonomic group, with 45% of the publications. The highest prevalence of the disease was registered for rodents (82.7%, 62/75), followed by humans (67.9%, 53/78), pigs (67.6%, 342/506), and dogs (67.2%, 41/61). The prevalence of the disease in the country is associated with occupational factors, hygiene conditions and contact with rodents. Conclusion: Although leptospirosis is a prevalent disease in Colombia, the limitations of the diagnostic techniques used and the lack of a unified criteria on titers thresholds, make an accurate assessment of the prevalence of the disease in the country problematic.

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Section: Discussionmentioning

confidence: 99%

“…Work at molecular level is revealing interesting results in various parts of the world and should be considered in future works [10][11][12][13][14][15][16].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A retrospective analysis of the leptospirosis research in Colombia

Dechner

2014

J Infect Dev Ctries

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“…Among molecular techniques, PCR and its different variations are highlighted as the most commonly used in laboratories and research institutes. Thus, these have contributed to identification and characterization of several organisms and understanding of physiopathology of diverse diseases in human, animal and plant [39,40]. Also these have provided clues for future research directions in specific topics with impact in public health such as genetics and biochemistry of antimicrobial resistance [41,42].…”

Section: Applications Of Pcr and Impact On Sciencementioning

confidence: 99%

Polymerase Chain Reaction: Types, Utilities and Limitations

Hernández-Rodríguez¹,

Ramírez²

2012

Polymerase Chain Reaction

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“…Leptospirosis occurs worldwide and is acquired through a direct or indirect contact with the urine of infected animals (2). According to many researchers (12,19), leptospirosis of cattle is widespread in the world and causes a considerable economic damage due to high lethality (25%-45% and more), drop in milk yields (by 23% -37%), body weight loss (by 18%-28%), young growth retardation, offspring death (up to 90%), abortion (15%-20%), deterioration in commercial quality of leather of animals affected, culling of livestock products in slaughter houses and meat processing plants, disturbance of reproductive functions, as well as spending significant means for diagnostic, preventive, medical, and quarantine-restrictive measures.…”

Section: Introductionmentioning

confidence: 99%

Leptospirosis in Cattle From Markets of Almaty Province, Kazakhstan

Kirkimbayeva

Łozowicka

Biyashev

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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This paper is the first study of the prevalence of leptospirosis in the cattle at slaughter from a rural area of Kazakhstan. Five hundred and seventy three samples of serum, urine, and kidneys from cattle of Alatau, Kazakh white and Auliyekol breed, aged from 2 to 5 years (unknown vaccination status), from the province of Almaty in the South-Eastern region were collected during four years (March 2010 to October 2013). The serological, bacteriological, and molecular analyses were performed. Serum samples were tested with 14 reference Leptospira serovars by microscopic agglutination test (MAT). MAT results showed that 89 (15.53%) serum samples had detectable antibodies against seven serovars of L. interrogans at a dilution of ≥1:100. Serovars: Pomona (38.2%), Tarassovi (27.2%), and Kabula (18.8%) were the most prevalent and their titres ranged from 100 to 1200. The spirochetes were detected in 11 samples of urine and nine samples of kidneys under dark-field microscope observation. The pure cultures were obtained from three samples. PCR technique confirmed leptospirosis in 23 out of 89 urine samples from cows, which showed the presence of leptospiral antibodies in microagglutination test. The high disease prevalence in cows indicates the high Leptospira contamination in this area. It was concluded that the bovine leptospirosis is an endemic and locally widespread disease in Kazakhstan, and that it may play a role in zoonotic transmission to humans.

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A comparison between polymerase chain reaction (PCR) and traditional techniques for the diagnosis of leptospirosis in bovines

Cited by 54 publications

References 18 publications

A retrospective analysis of the leptospirosis research in Colombia

A retrospective analysis of the leptospirosis research in Colombia

Polymerase Chain Reaction: Types, Utilities and Limitations

Leptospirosis in Cattle From Markets of Almaty Province, Kazakhstan

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