A comparison in germ‐free mice of the pathogenesis of Sendai virus and mouse pneumonia virus infections

Abstract

The poathogenesis of Sendai virus and pneumonia virus of mice (PVM) was studied using the immunoperoxidase technique on paraffin lung sections. The pathology of Sendai virus corresponds to that of a bronchopneumonia, with virus demonstrated by immunoperoxidase in the bronchial epithelium, and sometimes in macrophages, for a period of 2--9 days post-infection. Pneumonia virus of mice produces an interstitial pneumonia with virus demonstrated in the bronchial epithelium but also in the alveolar walls and alveolar macrophages. This virus can be demonstrated between 2 and 7 days post-infection. This technique was used to demonstrate PVM in the case of a natural outbreak of this disease and may eventually become a routine technique for the screening of lung tissue for respiratory viruses.

Pathogenicity of Sendai Viruses Adapted into Polarized MDCK Cells.

“…of virus. Their HA titers were 2 8 and 2 6 HA units and their PFU were 7.5 × 10 6 and 3.5 × 10 6 , respectively, while their egg-grown parental virus had a titer of 2 12 HA units and 4 × 10 9 PFU. In the MDCK cells, the apically and basally released virus showed almost the same plating efficiency as their parental strain, although the basally released MDCKadapted virus reached higher titer than the apically released MDCK-adapted virus and the egg-grown parental virus (Fig.…”

“…Interstitial pneumonia is characterized by the swelling and sloughing of type 1 and 2 alveolar epithelial cells with the occasional presence of multinucleated type 2 syncytial cells. The SeV are known to initiate damage resulting in the disorganization of alveolar parenchyma and fibrosis around terminal and alveolar ducts [5,6,8,21].…”

Animal Models of Respiratory Syncytial Virus Pathogenesis and Vaccine Development: Opportunities and Future Directions

“…PVM is a natural respiratory pathogen of murids, and laboratory mouse strains are highly susceptible to experimental PVM infection (Rosenberg et al, 2005). PVM challenge of mice showed that changes in bronchial epithelial cells were evident as early as day 1 post-infection and were characterized by granular changes in the cytoplasm with lifting and stripping of the bronchial epithelium and shedding of cellular debris into bronchial lumina (Carthew and Sparrow, 1980). Plugs of cellular debris and neutrophils were evident by day 4 postinfection, as were severe alveolar congestion and edema, with infiltration of neutrophils and macrophages into alveolar spaces and the interstitium.…”

Green Fluorescent Protein Gene Insertion of Sendai Virus Infection in Nude Mice: Possibility as an Infection Tracer.

“…progression of lung lesions with persistent SeV [4,12,20,23]. In this study, we compared the pathogenicity of recombinant SeV with an inserted GFP gene (GFP-SeV) with that of its recombinant wild-type SeV (Wt-SeV) to determine the usefulness of the recombinant virus application for studying viral pathogenicity.…”

“…4a). Until day 10, the majority of fluorescent cells within infected mice lack thymic T-lymphocyte cell [4,12,20,23,24], they have a subpopulation of T-lymphocytes in their lymph nodes and in compensation, they show hyperactivity of B cells and macrophages as an antiviral response during viral infection [10,18,19,21]. Therefore the outcome of the SeV infection could still be observed within the lungs, in different timing and degree of severity depending on the virulence.…”