A comparison of absorption inhibition and absorption elution methods for estimation of ABO blood groups in saliva

Sen, Manu Prasad; Vanishree, M; Hunasgi, Santosh; Surekha, R.; Koneru, Anila; Manvikar, Vardendra

doi:10.15713/ins.jmrps.1

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…Identification of blood group especially from saliva has been important in establishing the identification of criminals, victims in crime scenes. 4 In the year 1928 presence of anti-A and anti-B hemagglutinins was first analyzed in saliva. Because of inadequate procedures it was not in use.…”

Section: Discussionmentioning

confidence: 99%

“…3 There are two methods for detection of ABO blood group from saliva; they are absorption inhibition method and absorption-elution method. 4 The present study was conducted for the assessment of ABH secretor status from saliva by absorption inhibition method.…”

Section: Introductionmentioning

confidence: 99%

“… 3 There are two methods for detection of ABO blood group from saliva; they are absorption inhibition method and absorption-elution method. 4 …”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Tejasvi

Bukkya²,

Rao

et al. 2021

Global Medical Genetics

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Objectives While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Tejasvi

Bukkya²,

Rao

et al. 2021

Global Medical Genetics

View full text Add to dashboard Cite

show abstract

“… 11 Moreover, many studies have correlated the secretor status and many systemic illnesses like ankylosing spondylitis, peptic ulcer, ovarian cysts, and even squamous cell carcinoma. 12 Thus, dried salivary samples proved to be a reliable source for ABO blood grouping; however, for the detection of D antigen, a more technique-sensitive approach needs to be developed.…”

Section: Discussionmentioning

confidence: 99%

Determination of ABO Blood Groups and Rh Typing from Dry Salivary Samples

Velani

Shah

Lakade

2018

International Journal of Clinical Pediatric Dentistry

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IntroductionA unique blood group is a characteristic every individual possesses. Blood group antigens like A, B, D, H, etc., are found to be present on the cell surfaces of red blood cells (RBCs). Besides blood, these are also secreted in various body secretions like semen, sweat, amniotic fluid, and saliva. Blood grouping has several applications in forensic sciences and is also a major part of routine medical investigations. Presence of these antigens in saliva is dependent on the secretor status of an individual. Saliva samples at the crime scene are very crucial, as they help in deoxyribonucleic acid typing, sex determination, bite mark analysis, and blood grouping. Dried salivary samples are often obtained in more number of cases as compared with the wet form, due to the variable time lapse between the occurrence of the crime and the start of the investigation. Blood grouping from these samples proves to be very efficient. Thus, the present study aims at evaluating the accuracy of ABO blood group determination and Rh typing from dried salivary samples. Also, the study would establish the use of saliva as a noninvasive technique in routine blood examinations, especially in children who have needle phobia.Materials and methodsBlood grouping and Rh typing were performed on the dry salivary samples obtained from the 47 subjects using the absorption-inhibition technique. This was then compared with the results obtained using extraction socket blood and evaluated.ResultsThe present study showed a 100% positive correlation for ABO blood grouping, but a mere 14.81% positive correlation for Rh typing between the dried salivary samples and the extraction socket blood.ConclusionDried salivary samples can thus be put to immense use in several areas of forensic investigations. It could also help in developing alternate methods for routine blood investigations in children and adults.How to cite this article: Velani PR, Shah P, Lakade L. Determination of ABO Blood Groups and Rh Typing from Dry Salivary Samples. Int J Clin Pediatr Dent 2018;11(2):100-104.

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“…The PM changes in the pulp are seen very late, and the pulp remains protected in the tooth cavity, thus making the pulp tissues one of the best agents for blood group recognition. Various studies done by absorptionelution or the absorption-inhibition methods are used for ABO blood grouping and Rh typing [21,[23][24]. Another study by Aswath et al was conducted to concentrate on the sensitivity and specificity of the dental pulp in identifying the ABO blood group and Rh factor [25,26].…”

Section: The Use Of Tooth Dna In Blood Groupingmentioning

confidence: 99%

Forensic Odontology, a Boon and a Humanitarian Tool: A Literature Review

Kaleelullah

Hamid

2020

Cureus

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Our society faces fresh challenges in every conceivable way, from an increase in the crime rate to a rise in natural disasters. In spite of the leaps in modern technology, medical breakthroughs in the identification of criminals remain a cumbersome task. The modern-day criminal investigation typically involves many different disciplines to solve a crime. The identification of a person's body is required when it is completely mutilated, disfigured, or beyond recognition-which might be due to any barbaric crime, accident, war, fire, or any natural disaster. Dental identification is one of the most reliable methods, as teeth and dental structures may survive adverse conditions. The techniques involved in forensic odontology include: bite mark analysis, tooth prints, rugroscopy, cheiloscopy, dental DNA analysis, radiographs, and photographs.

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A comparison of absorption inhibition and absorption elution methods for estimation of ABO blood groups in saliva

Cited by 10 publications

References 8 publications

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Determination of ABO Blood Groups and Rh Typing from Dry Salivary Samples

Forensic Odontology, a Boon and a Humanitarian Tool: A Literature Review

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