A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat

Ohe, C. von der; Gauthier, Victoria; Tamburic-Ilincic, L.; Brûlé‐Babel, Anita L.; Fernando, W. G. Dilantha; Clear, R. M.; Ward, Todd J.; Miedaner, Thomas

doi:10.1007/s10658-010-9607-z

Cited by 81 publications

(52 citation statements)

References 30 publications

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“…However, potential strain or population-specific differences in the total amount of trichothecene accumulated in grain is also of concern. In analyses of Canadian F. graminearum, 3-ADON strains accumulated significantly more DON in vivo than 15-ADON strains (von der Ohe et al 2010;Ward et al 2008). The prevalence of 3-ADON among Norwegian F. graminearum could therefore indicate a relatively larger threat to food safety in comparison to regions in which 15-ADON isolates predominate.…”

Section: Discussionmentioning

confidence: 96%

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

et al. 2015

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As has been observed in several European countries, the frequency of Fusarium head blight (FHB) caused by members of the Fusarium graminearum species complex (FGSC) has increased in Norwegian cereals in recent years, resulting in elevated levels of deoxynivalenol in cereal grains. The objective of this study was to determine if this increase was associated with changes in FGSC composition within Norway. FGSC isolates collected from wheat, oats and barley in Norway during two periods, mainly 1993-1998 and 2004-2007, were characterized to determine species and trichothecene genotype composition and to assess levels of genetic variation and population structure. In vitro growth rates at different temperatures and aggressiveness in spring wheat were further characterized for a sub-selection of isolates. All Norwegian isolates were identified as F. graminearum. The 3-acetyl-deoxynivalenol (3-ADON) trichothecene type was dominant. However, isolates with the 15-ADON chemotype were detected in Norway for the first time and may represent a recent introduction of this trichothecene type. Bayesian-model based clustering and analyses of genetic differentiation indicated the persistence over the last 20 years of two sympatric and partially admixed populations of F. graminearum in Norway. Significant differences in average in vitro growth rates and aggressiveness were observed between these two populations. Our results demonstrate that the recent increase in prevalence of the FGSC in Norwegian cereals do not correspond to any dramatic changes in FGSC species or trichothecene chemotype composition. However, significant changes in population frequencies were observed among Norwegian F. graminearum.

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Section: Discussionmentioning

confidence: 96%

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

et al. 2015

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“…Gilbert et al (2010) reported that 15-ADON and 3-ADON chemotypes did not differ in their pathogenicity, but the 3-ADON chemotypes produced more DON, on average, than 15-ADON chemotypes. Von der Ohe et al (2010) also found that the 3-ADON chemotypes produced more DON than 15-ADON chemotypes, after inoculation, in wheat that was rated as moderately resistant. Puri and Zhong (2010) reported that 3-ADON chemotypes in the laboratory produced more DON and conidia compared with 15-ADON chemotypes.…”

Section: Mots Clé Smentioning

confidence: 95%

“…Fusarium graminearum produce DON, nivalenol (NIV) and zearalenone, as well as, 3-acetyl deoxynivalenol (3-ADON), 15-acetyl deoxynivalenol (15-ADON) (Ichinoe et al 1983;Miller et al 1991;O'Donnell et al 2000). However, some 15-ADON chemotypes produced both 15-ADON and 3-ADON in grain after inoculation (Von der Ohe et al 2010). Fusarium poae and F. sporotrichioides have been reported to be capable of forming HT-2, and T-2 (Foroud and Eudes 2009).…”

Section: Mots Clé Smentioning

confidence: 99%

Mycotoxin accumulation and Fusarium graminearum chemotype diversity in winter wheat grown in southwestern Ontario

2015

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Tamburic-Ilincic, L., Wragg, A. and Schaafsma, A. 2015. Mycotoxin accumulation and Fusarium graminearum chemotype diversity in winter wheat grown in southwestern Ontario. Can. J. Plant Sci. 95: 931–938. Fusarium head blight caused by Fusarium graminearum is a serious disease of wheat (Triticum aestivum L.). Deoxynivalenol (DON) is the mycotoxin most commonly detected in contaminated wheat grain in Ontario. A chemotype shift from 15-acetyldeoxynivalenol (15-ADON) to 3-acetyldeoxynivalenol (3-ADON) has been reported in some parts of North America. The objectives of this study were to investigate the mycotoxins accumulation and Fusarium graminearum chemotype diversity in different winter wheat cultivars grown in southwestern (SW) Ontario. Twenty winter wheat grain samples from SW Ontario were tested for DON concentrations in 2008, 24 in each of 2009 and 2010, 42 in 2011 and 36 in 2013 growing seasons. DON levels in grain ranged from none detected to 33 parts per million (ppm). The highest averaged levels of DON were detected in the 2013 season (19.8 ppm at Centralia, 11.8 ppm at Inwood and 4.1 ppm at Ridgetown). In addition to total DON, H-T2, T-2 and nivalenol mycotoxins were detected in low concentrations in a small number of samples collected in 2009 and/or 2013. The 15-ADON analogs were detected at approximately 1.5% of the total DON in harvested wheat grain in 2013. Ninety-seven percent of F. graminearum isolates, tested in 2010 using the polymerase chain reaction (PCR) method, were 15-ADON chemotype, while just 3% of isolates were the 3-ADON chemotype. Overall, there was no evidence in this study to support a shift from 15-ADON to 3-ADON chemotypes of F. graminearum in SW Ontario in 2010. It is recommended to continue monitoring mycotoxin accumulation and F. graminearum chemotype diversity so any changes can be detected early and addressed.

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“…chemotype in resistant and susceptible wheat cultivars. [12,48] The increase in the incidence of F. graminearum and F. culmorum populations in the eastern Mediterranean region of Turkey could potentially have resulted in increased levels of DON and reduced levels of NIV contamination in wheat grains, depending on the toxin profile of both Fusarium species. However, the high proportion of DON chemotype, found within the F. graminearum and F. culmorum populations, as reported here, indicated that DON contamination was high and NIV contamination was low in wheat grains in the eastern Mediterranean region of Turkey.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Distribution and genetic chemotyping ofFusarium graminearumandFusarium culmorumpopulations in wheat fields in the eastern Mediterranean region of Turkey

Tok

Arslan

2016

Biotechnology & Biotechnological Equipment

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Fusarium graminearum and Fusarium culmorum are among the major causal agents of Fusarium head blight, which reduces both crop yield and grain quality in wheat worldwide. The present study was conducted with 57 isolates collected from 23 different locations across four provinces in the 2011/2012 growing season. Out of the 57 Fusarium isolates, 32 isolates were identified as F. graminearum and 25 isolates were identified as F. culmorum. Both pathogens are of particular importance, since they produce several mycotoxins. Among these, deoxynivalenol (DON) and nivalenol (NIV) are well known for their toxicity towards human and animal health. Genetic chemotyping of F. graminearum and F. culmorum species indicated that both DON and NIV chemotypes were present in the surveyed area. Of the 32 F. graminearum isolates, the primer sets Tri13DON and Tri13NIV identified 87.5% as DON chemotypes and 12.5% as NIV chemotypes. Similarly, the 25 F. culmorum isolates displayed 88% DON and 12% NIV chemotypes. In addition, DON acetylated derivatives, 3-acetyldeoxynivalenol (3-AcDON) and 15-AcDON, were identified by polymerase chain reaction based methods. It was determined that 15-AcDON sub-chemotype was dominant in F. graminearum populations, whereas 3-AcDON was dominant in F. culmorum populations. This is the first report demonstrating the presence of F. graminearum and F. culmorum isolates and the distribution of 3-AcDON and 15-AcDON chemotypes in both Fusarium species in wheat fields of eastern Mediterranean region of Turkey.

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A comparison of aggressiveness and deoxynivalenol production between Canadian Fusarium graminearum isolates with 3-acetyl and 15-acetyldeoxynivalenol chemotypes in field-grown spring wheat

Cited by 81 publications

References 30 publications

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway

Mycotoxin accumulation and Fusarium graminearum chemotype diversity in winter wheat grown in southwestern Ontario

Distribution and genetic chemotyping ofFusarium graminearumandFusarium culmorumpopulations in wheat fields in the eastern Mediterranean region of Turkey

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