A Comparison of Biological Coatings for the Promotion of Corneal Epithelialization of Synthetic Surface In Vivo

Sweeney, Deborah F.; Xie, Ruo Zhong; Evans, Margaret D. M.; Vannas, Antti; Tout, S.; Griesser, Hans J.; Johnson, Graham; Steele, John G.

doi:10.1167/iovs.02-0561

Cited by 41 publications

(37 citation statements)

References 43 publications

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“…The volumes of the solution in the two-compartment chambers were both 250 mL (V) and the thickness of the wet film was 110 ± 5 μm (d). On the basis of the above formulas, the diffusion coefficient of the samples to the NaCl solution was about 2.43 × 10 −6 cm 2 /s, which is comparable to that of the human cornea (2.5 × 10 −6 cm 2 /s) [23]. The diffusion coefficient of the films to the tryptophan solution was 7.97× 10 −7 cm 2 /s, which is also a stable diffusion.…”

Section: Resultsmentioning

confidence: 69%

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Liu

Wang

2013

Materials Science and Engineering: C

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Section: Resultsmentioning

confidence: 69%

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Liu

Wang

2013

Materials Science and Engineering: C

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“…Promotion of cell adhesion to polymers is most often accomplished by the incorporation of bioactive peptides and proteins through covalent immobilization. The extracellular matrix proteins collagen, fibronectin, and laminin as well as their peptide derivatives such as RGD and YIGSR, have been incorporated into various artificial cornea constructs to mimic the basement membrane of the natural corneal epithelium (Aucoin et al, 2002;Sweeney et al, 2003). While many hydrogel modification strategies involve the incorporation of adhesion-promoting ligands during polymerization, these do not allow for high concentrations of proteins at the tissue-implant interface because a large proportion of the ligands are inside the polymer and inaccessible to cells.…”

Section: Discussionmentioning

confidence: 99%

Design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct

Myung

Koh

Bakri

et al. 2007

Biomed Microdevices

110

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We describe the design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct, and demonstrate the adhesion of corneal epithelial and fibroblast cells to its central and peripheral components, respectively. The design consists of a central "core" optical component and a peripheral tissue-integrable "skirt." The core is composed of a poly(ethylene glycol)/poly(acrylic acid) (PEG/PAA) double-network with high strength, high water content, and collagen type I tethered to its surface. Interpenetrating the periphery of the core is a microperforated, but resilient poly(hydroxyethyl acrylate) (PHEA) hydrogel skirt that is also surface-modified with collagen type I. The well-defined microperforations in the peripheral component were created by photolithography using a mask with radially arranged chrome discs. Surface modification of both the core and skirt elements was accomplished through the use of a photoreactive, heterobifunctional crosslinker. Primary corneal epithelial cells were cultured onto modified and unmodified PEG/PAA hydrogels to evaluate whether the central optic material could support epithelialization. Primary corneal fibroblasts were seeded onto the PHEA hydrogels to evaluate whether the peripheral skirt material could support the adhesion of corneal stromal cells. Cell growth in both cases was shown to be contingent on the covalent tethering of collagen. Successful demonstration of cell growth on the two engineered components was followed by fabrication of core-skirt constructs in which the central optic and peripheral skirt were synthesized in sequence and joined by an interpenetrating diffusion zone.

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“…15 In addition to the biophysical attributes of substrates, their integral surface chemistry has been intensively investigated as it relates to the modulation of cell behaviors. [16][17][18][19][20] In vivo, ECM proteins such as laminin, collagen, and fibronectin (FN) are among the protein constituents that form the rich 3D topographic landscape with which cells intimately associate. Among the most important and widely studied ECM proteins/peptides that influence the cellsubstratum interaction are FN, collagen (C), and the ArgGly-Asp (RGD)-containing peptides.…”

Section: Introductionmentioning

confidence: 99%

Influence of Extracellular Matrix Proteins and Substratum Topography on Corneal Epithelial Cell Alignment and Migration

Raghunathan

McKee

Cheung

et al. 2013

Tissue Engineering Part A

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The basement membrane (BM) of the corneal epithelium presents biophysical cues in the form of topography and compliance that can impact the phenotype and behaviors of cells and their nuclei through modulation of cytoskeletal dynamics. In addition, it is also well known that the intrinsic biochemical attributes of BMs can modulate cell behaviors. In this study, the influence of the combination of exogenous coating of extracellular matrix proteins (ECM) (fibronectin-collagen [FNC]) with substratum topography was investigated on cytoskeletal architecture as well as alignment and migration of immortalized corneal epithelial cells. In the absence of FNC coating, a significantly greater percentage of cells aligned parallel with the long axis of the underlying anisotropically ordered topographic features; however, their ability to migrate was impaired. Additionally, changes in the surface area, elongation, and orientation of cytoskeletal elements were differentially influenced by the presence or absence of FNC. These results suggest that the effects of topographic cues on cells are modulated by the presence of surface-associated ECM proteins. These findings have relevance to experiments using cell cultureware with biomimetic biophysical attributes as well as the integration of biophysical cues in tissueengineering strategies and the development of improved prosthetics.

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A Comparison of Biological Coatings for the Promotion of Corneal Epithelialization of Synthetic Surface In Vivo

Abstract: A biologically modified polymer can support migration and adhesion of corneal epithelial cells in vivo. The collagen I-modified surface exhibited the most promising performance, both clinically and histologically.

Cited by 41 publications

References 43 publications

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Crosslinked collagen–gelatin–hyaluronic acid biomimetic film for cornea tissue engineering applications

Design and fabrication of an artificial cornea based on a photolithographically patterned hydrogel construct

Influence of Extracellular Matrix Proteins and Substratum Topography on Corneal Epithelial Cell Alignment and Migration

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