A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity

Bertoncello, Ivan

doi:10.1016/b978-0-12-564504-1.50008-8

Cited by 4 publications

(3 citation statements)

References 61 publications

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“…With respect to the effect of a nonspherical cell shape on the calculation of cell diameter from settling velocity measurements, Meistrich (1983) states that shape factors do not vary much between different types of cells, and even in an extreme case, such as for erythrocytes, which are ellipsoid, only a 10% effect is observed. Also, Bertoncello (1987), when comparing unit gravity sedimentation and centrifugal elutriation, states that shape factors may play a significant role in centrifugal elutiration, when compared to unit gravity sedimentation, since cells are placed under significant conformational stress as compared to the gentle forces in unit gravity sedimentation. A number of improvements in this system are being implemented and/or considered.…”

Section: Discussionmentioning

confidence: 99%

“…Two commercial devices that can be used for both cell size analysis and separation are unit gravity sedimentation and counterflow centrifugation (or centrifugal elutriation). The first device is based on gravity, while the second device relies on a balance between centrifugual sedimentation and centripetal flow (Bertoncello, 1987). With respect to unit gravity sedimentation, the terminal velocity, v t , is given by…”

Section: Introductionmentioning

confidence: 99%

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Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Chalmers

Haam

Zhao

et al. 1999

Biotechnol. Bioeng.

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An experimental technique is discussed in which the size distribution of a population of cells is determined by calculating each cell's settling velocity. The settling velocity is determined from microscopically obtained images which were recorded on SVHS tape. These images are then computer imaged and processed, and the cell's location and velocity are determined using a computer algorithm referred to as cell tracking velocimetry (CTV). Experimental data is presented comparing the distribution of human lymphocytes and a human breast cancer cell line, MCF‐7, determined using a Coulter counter and the CTV approach. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 509–518, 1999.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Chalmers

Haam

Zhao

et al. 1999

Biotechnol. Bioeng.

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show abstract

“…41,42 The technique of centrifugal elutriation for cell size determination is based on a balance between centrifugual sedimentation and centripetal flow. 43,44 By the method of centrifugal elutriation, the authors in Ref. 44 have calculated sizes of some human peripheral blood and bone marrow cells.…”

Section: Introductionmentioning

confidence: 99%

Investigation of morphometric parameters for granulocytes and lymphocytes as applied to a solution of direct and inverse light-scattering problems

et al. 2007

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Quantitative data on cell structure, shape, and size distribution are obtained by optical measurement of normal peripheral blood granulocytes and lymphocytes in a cell suspension. The cell nuclei are measured in situ. The distribution laws of the cell and nuclei sizes are estimated. The data gained are synthesized to construct morphometric models of a segmented neutrophilic granulocyte and a lymphocyte. Models of interrelation between the cell and nucleus metric characteristics for granulocyte and lymphocyte are obtained. The discovered interrelation decreases the amount of cell-nucleus size combinations that have to be considered under simulation of cell scattering patterns. It allows faster analysis of light scattering to discriminate cells in a real-time scale. Our morphometric data meet the requirements of scanning flow cytometry dealing with the high rate analysis of cells in suspension. Our findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in scanning flow cytometry, dispensing with a costly and time-consuming immunophenotyping of the cells, as well as in turbidimetry and nephelometry. The cell models developed can ensure better interpretations of scattering patterns for an improvement of discriminating capabilities of immunophenotyping-free scanning flow cytometry.

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Enrichment and Isolation Techniques for Animal Cell Types

Absher¹

2000

Encyclopedia of Cell Technology

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A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity

Cited by 4 publications

References 61 publications

Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Investigation of morphometric parameters for granulocytes and lymphocytes as applied to a solution of direct and inverse light-scattering problems

Enrichment and Isolation Techniques for Animal Cell Types

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