Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Chalmers, Jeffrey J.; Haam, Seungjoo; Zhao, Yunhui; McCloskey, Kara E.; Moore, Lee R.; Zborowski, Maciej; Williams, P. Stephen

doi:10.1002/(sici)1097-0290(19990905)64:5<509::aid-bit1>3.0.co;2-z

Cited by 26 publications

(31 citation statements)

References 16 publications

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“…As has been reported in a previous publication (Chalmers et al, 1999b;Jin et al, 2008), the settling velocity of the cells and the polystyrene microspheres can be related to the density difference between the cells (or microspheres) and the cell diameter by:…”

Section: Intrinsic Magnetophoretic Mobilitymentioning

confidence: 71%

“…We are not aware of any reported density values for Detriot 562 or Cal 27; however, we previous reported that the density difference for one cancer cell line, MCF-7, is 30 kg/m 3 (Chalmers et al 1999b). …”

Section: Resultsmentioning

confidence: 98%

“…Using Equation (10), the company reported polystyrene bead diameter of 4.5 mm, and a viscosity of 0.93 Â 10 À3 kg/m s, an average density of difference of these polystyrene beads in the buffer is determined to be 48 kg/m 3 . We are not aware of any reported density values for Detriot 562 or Cal 27; however, we previous reported that the density difference for one cancer cell line, MCF-7, is 30 kg/m 3 (Chalmers et al, 1999b).…”

Section: Intrinsic Magnetophoretic Mobilitymentioning

confidence: 95%

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Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection

Chalmers

Xiong

Jin

et al. 2010

Biotech & Bioengineering

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The maturation of magnetic cell separation technology places increasing demands on magnetic cell separation performance. While a number of factors can cause suboptimal performance, one of the major challenges can be non-specific binding of magnetic nano or micro particles to non-targeted cells. Depending on the type of separation, this non-specific binding can have a negative effect on the final purity, the recovery of the targeted cells, or both. In this work, we quantitatively demonstrate that non-specific binding of magnetic nanoparticles can impart a magnetization to cells such that these cells can be retained in a separation column and thus negatively impact the purity of the final product and the recovery of the desired cells. Through experimental data and theoretical arguments, we demonstrate that the number of MACS magnetic particles needed to impart a magnetization that is sufficient to causes non-targeted cells to be retained in the column to be on the order of 500 to 1,000 nanoparticles. This number of non-specifically bound particles was demonstrated experimentally with an instrument, cell tracking velocimeter, CTV, and it is demonstrated that the sensitivity of the CTV instrument for Fe atoms contained in magnetic nanoparticles on the order of 1 × 10−15 g/mL of Fe.

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Section: Intrinsic Magnetophoretic Mobilitymentioning

confidence: 71%

Section: Resultsmentioning

confidence: 98%

Section: Intrinsic Magnetophoretic Mobilitymentioning

confidence: 95%

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Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection

Chalmers

Xiong

Jin

et al. 2010

Biotech & Bioengineering

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show abstract

“…The differences are likely related to the cell density estimation, nonuniform density distribution in the cell population, and the departure from spherical cell shape (assumed in Eq. 6) (42). MSC mean diameter was 12.7 ± 2.1 μm, cardiac fibroblast mean diameter was 11.5 ± 1.6 μm, and KG‐1a cell mean diameter was 10.4 ±1.7 μm.…”

Section: Methodsmentioning

confidence: 99%

Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis

Jing

Mal²,

Williams

et al. 2008

FASEB j.

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Superparamagnetic iron oxide (SPIO) particles have been used successfully as an intracellular contrast agent for nuclear MRI cell tracking in vivo. We present a method of detecting intracellular SPIO colloid uptake in live cells using cell magnetophoresis, with potential applications in measuring intracellular MRI contrast uptake. The method was evaluated by measuring shifts in mean and distribution of the cell magnetophoretic mobility, and the concomitant changes in population frequency of the magnetically positive cells when compared to the unmanipulated negative control. Seven different transfection agent (TA) -SPIO complexes based on dendrimer, lipid, and polyethylenimine compounds were used as test standards, in combination with 3 different cell types: mesenchymal stem cells, cardiac fibroblasts, and cultured KG-1a hematopoietic stem cells. Transfectol (TRA) -SPIO incubation resulted in the highest frequency of magnetically positive cells (>90%), and Fugene 6 (FUG) -SPIO incubation the lowest, below that when using SPIO alone. A highly regular process of cell magnetophoresis was amenable to intracellular iron mass calculations. The results were consistent in all the cell types studied and with other reports. The cell magnetophoresis depends on the presence of high-spin iron species and is therefore expected to be directly related to the cell MRI contrast level.

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“…This method is useful for trapping cells having surface markers but it cannot discriminate between cells having varying densities of surface markers. To confer marker density discrimination, continuous magnetophoretic (MAP) sorting of immunomagnetically labeled cells in a laminar flow profile has been demonstrated [82], [83]. In this scheme, cells are deflected by a quadrapole magnetic field in proportion to the degree of labeling and emerge from the sorting chamber along a trajectory that allows their differential recovery.…”

Section: ) Dielectrophoretic-magnetophoretic-fff (Map-dep-fff)mentioning

confidence: 99%

Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments

2004

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Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

Cited by 26 publications

References 16 publications

Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection

Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection

Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis

Dielectrophoresis-Based Sample Handling in General-Purpose Programmable Diagnostic Instruments

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