Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection

Chalmers, Jeffrey J.; Xiong, Ying; Jin, Xinqiao; Shao, Mingwang; Tong, Xiaodong; Farag, Sherif; Zborowski, Maciej

doi:10.1002/bit.22635

Cited by 37 publications

(40 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This highlights the benefits of this technique as it is sensitive not only to the bulk amount of iron taken up by cells, but also the cell's magnetisation, which arises from SPION uptake and their physical properties. 48 Importantly, we have not seen any changes in cell viability when cells were labelled with the different SPIONs (Fig. 5B), suggesting no cytotoxic effects originating from these materials at the concentrations and timescale studied here.…”

Section: Resultsmentioning

confidence: 80%

Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast

et al. 2015

View full text Add to dashboard Cite

Tracking stem cells in vivo using non-invasive techniques is critical to evaluate the efficacy and safety of stem cell therapies. Superparamagnetic iron oxide nanoparticles (SPIONs) enable cells to be tracked using magnetic resonance imaging (MRI), but to obtain detectable signal cells need to be labelled with a sufficient amount of iron oxide. For the majority of SPIONs, this can only be obtained with the use of transfection agents, which can adversely affect cell health. Here, we have synthesised a library of dextran-based polymer coated SPIONs with varying surface charge from -1.5 mV to +18.2 mV via a co-precipitation approach and investigated their ability to be directly internalised by stem cells without the need for transfection agents. The SPIONs were colloidally stable in physiological solutions. The crystalline phase of the particles was confirmed with powder X-ray diffraction and their magnetic properties were characterised using SQUID magnetometry and magnetic resonance. Increased surface charge led to six-fold increase in uptake of particles into stem cells and higher MRI contrast, with negligible change in cell viability. Cell tracking velocimetry was shown to be a more accurate method for predicting MRI contrast of stem cells compared to measuring iron oxide uptake through conventional bulk iron quantification.

show abstract

Section: Resultsmentioning

confidence: 80%

Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast

et al. 2015

View full text Add to dashboard Cite

show abstract

“…A third factor in the selection of magnetic particles for cell separation applications that has caused some debate amongst researchers and commercial particle vendors is the influence of particle size on cell fate and characteristics. It is well know that nanoparticles attached to cells will, over a span of a few hours to days, will be taken into the cell cytoplasm through a process called endocytosis (Iversen et al, 2011, Chalmers et al, 2010, Jing et al, 2008). This phenomenon results in the accumulation of a population of nanoparticles within the cells, affecting the internal biology of the cell.…”

Section: Magnetophoretic Cell Separation: Theory and Phenomenamentioning

confidence: 99%

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

View full text Add to dashboard Cite

Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell separation systems.

show abstract

“…32–35 Furthermore, the non-specific off-target cell binding and uptake of nanoparticles by non-target cells make differentiating CTCs from normal cells difficult. 36–39 Therefore, an approach to eliminate or reduce the non-specific interactions between nanoparticles and the unwanted materials and cells is highly desirable for applying immuno-magnetic separation techniques to CTC detection.…”

Section: Introductionmentioning

confidence: 99%

Improving sensitivity and specificity of capturing and detecting targeted cancer cells with anti-biofouling polymer coated magnetic iron oxide nanoparticles

Lin

MacDonald

et al. 2017

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Detecting circulating tumor cells (CTCs) with high sensitivity and specificity is critical to management of metastatic cancers. Although immuno-magnetic technology for in vitro detection of CTCs has shown promising potential for clinical applications, the biofouling effect, i.e., non-specific adhesion of biomolecules and non-cancerous cells in complex biological samples to the surface of a device/probe, can reduce the sensitivity and specificity of cell detection. Reported herein is the application of anti-biofouling polyethylene glycol-block-allyl glycidyl ether copolymer (PEG-b-AGE) coated iron oxide nanoparticles (IONPs) to improve the separation of targeted tumor cells from aqueous phase in an external magnetic field. PEG-b-AGE coated IONPs conjugated with transferrin (Tf) exhibited significant anti-biofouling properties against non-specific protein adsorption and off-target cell uptake, thus substantially enhancing the ability to target and separate transferrin receptor (TfR) over-expressed D556 medulloblastoma cells. Tf conjugated PEG-b-AGE coated IONPs exhibited a high capture rate of targeted tumor cells (D556 medulloblastoma cell) in cell media (58.7 ± 6.4%) when separating 100 targeted tumor cells from 1×105 non-targeted cells and 41 targeted tumor cells from 100 D556 medulloblastoma cells spiked into 1 mL blood. It is demonstrated that developed nanoparticle has higher efficiency in capturing targeted cells than widely used micron-sized particles (i.e., Dynabeads®).

show abstract

Quantification of non‐specific binding of magnetic micro‐ and nanoparticles using cell tracking velocimetry: Implication for magnetic cell separation and detection

Cited by 37 publications

References 19 publications

Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast

Tailoring the surface charge of dextran-based polymer coated SPIONs for modulated stem cell uptake and MRI contrast

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

Improving sensitivity and specificity of capturing and detecting targeted cancer cells with anti-biofouling polymer coated magnetic iron oxide nanoparticles

Contact Info

Product

Resources

About