A comparison of different methods of red blood cell leukoreduction and additive solutions on the accumulation of neutrophil‐priming activity during storage

Abstract

Although both filtration and combination LR decrease the accumulation of proinflammatory activity versus buffy coat LR, combination LR is not more advantageous over filtration, has increased costs, and may cause increased hemolysis. In addition, AS-3 decreases the early accumulation of PMN-priming activity during storage versus AS-1 or AS-5.

Antimicrobial blue light for decontamination of platelets during storage

“…Bacterial contamination of PLT concentrates during storage poses the greatest risk of bacterial sepsis and fatality in modern transfusion medicine due to unique storage conditions required for PLTs . While most of human blood products can be stored in refrigerators or freezers where microbial contamination can be well under control , PLTs are uniquely stored at room temperature (20°C‐24°C) with continuous agitation around 60 rpm/minute so as to sustain their survival and functionality . However, room temperature favors the growth of various microorganisms, which is one of the main reasons to limit PLT storage for 5 days only in the United States, although PLTs are permitted to store for up to 7 days in the United Kingdom and Canada with pathogen inactivation technologies or in the United States in a 7‐day container with bacterial detection devices approved by FDA .…”

The lysophospholipid‐binding molecule CD1D is not required for the alloimmunization response to fresh or stored RBCs in mice despite RBC storage driving alterations in lysophospholipids

“…Lysophospholipids are an important class of bioactive lipids that are known to have multiple different downstream effects on immune cell function, and other bioactive lipids have been shown to be altered during RBC storage. 7,8 We employed a mass spectrometry-based approach to characterize the changes in lysophospholipids that are induced during storage of RBCs from the background strain of HOD mice, FVB. Although there were small but reproducible alterations for many of the lipid species measured (Figure 2), four lysophospholipid species demonstrated greater than twofold changes in their abundance after RBC storage.…”

“…One species of lysophosphatidylcholine (LPC) was decreased twofold: LPC (20:4), and one species LPE was decreased fourfold: LPE (18:0). Although there are several publications that have looked at lipid profiles of stored RBCs, [6][7][8][31][32][33][34] we have uniquely quantified a wide range of different lysophospholipid species of different acyl chain lengths. Collectively, our MS data demonstrate that storage of RBCs induces a range of changes in multiple different lysophospholipid species with some increasing while others decreasing.…”

“…RBC storage has been shown to induce a wide variety of biophysical and molecular changes, 6 and several reports have demonstrated that storage of human RBCs leads to production of biologically active lipids capable of activating neutrophils. 7,8 Thus, potential sources of DAMPs are bioactive lipids such as lysophospholipids.…”

“…DAMPs are endogenous molecules that are released from damaged cells and are recognized by specific receptors that can in turn stimulate the immune system. RBC storage has been shown to induce a wide variety of biophysical and molecular changes, 6 and several reports have demonstrated that storage of human RBCs leads to production of biologically active lipids capable of activating neutrophils 7,8 . Thus, potential sources of DAMPs are bioactive lipids such as lysophospholipids.…”