A Comparison of Direct and Indirect Somatic Morphogenesis for the Production of Transgenic Sugarcane (Saccharum Spp. Hybrids)

Snyman, S. J.; Huckett, B. I.; Botha, Frederik C.; Watt, M. P.

doi:10.17660/actahortic.2001.560.13

Cited by 22 publications

(35 citation statements)

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“…It might have been due to the formation of polyphenols in pith cultures, which resulted in poor callus formation. Our results are in accordance with the findings of Aftab and lqbal (1999) and Snyman et al (2001).…”

Section: Callogenesis In Response To 24-d In Ms Mediumsupporting

confidence: 93%

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

Shahid¹,

Khan²,

Saeed³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog medium. Two types of explants, leaf and pith, and two auxins, 2,4-dichlorophenoxy acetic acid and indole-3-acetic acid, were tested to optimize callogenesis for root establishment. Leaves as explants with 3.0 mg/L 2,4-dichlorophenoxy acetic acid gave the best results, both for callus induction and proliferation. Half-strength Murashige and Skoog medium with 1.5 mg/L indole-3-butyric acid proved to be the best for rooting. Red rot-resistant somaclones of the R 2 generation along with the parent were assessed for genetic variability at the molecular level using RAPD and SSR markers. Polymorphism based on RAPD and SSR was 32 and 67%, respectively. Polymorphic information content ranged from 0.06-0.45 for RAPD and 0.06-0.47 for SSR. We conclude that somaclonal variation of sugarcane varieties is sufficient to allow selection.

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Section: Callogenesis In Response To 24-d In Ms Mediumsupporting

confidence: 93%

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

Shahid¹,

Khan²,

Saeed³

et al. 2011

Genet. Mol. Res.

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“…This marked a major milestone in sugarcane transformation. Further re fi nements in tissue culture media and explant preparation and improvements in biolistic transfer protocols allowed transformation of sugarcane to become fairly routine in many laboratories (Tables 11.1 and 11.2 ), using easily established target tissues, including embryogenic callus (Bower et al 1996 ;Falco et al 2000 ;Gallo-Meagher and Irvine 1996 ;Gilbert et al 2005 ;Ingelbrecht et al 1999 ;Joyce et al 1998 ;Snyman et al 1996 ;Weng et al 2006 ;Zhang et al 1999 ) and immature leaf roll disk explants (Snyman et al 2000(Snyman et al , 2006 . Agrobacterium -mediated gene transfer was successful in sugarcane for a limited number of varieties and target tissues such as embryogenic calli (Arencibia et al 1998 ;Elliott et al 1998 ) , immature leaf sections (Enríquez-Obregón et al 1997 , and axillary meristems (Manickavasagam et al 2004 ) .…”

Section: Historical Perspectivementioning

confidence: 99%

Genetic Engineering of Saccharum

Beyene

Curtis

Damaj

et al. 2012

Genomics of the Saccharinae

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Over the last two decades, substantial progress has been made in the genetic engineering of sugarcane ( Saccharum spp.) through improvements in tissue culture procedures, allowing a higher ef fi ciency of generating transgenic plants using Agrobacterium -mediated and biolistic gene transfers. Elucidation of gene function and development of varieties with improved yield, sugar level, fi ber content, and other desirable traits and products for superior performance have been possible through transgenic technologies. Researchers are now focusing on optimizing existing methodologies and developing new technologies for the production of elite varieties, enhancement of transgene expression, and manipulation of metabolic pathways for improved molecular breeding and commercial exploitation. At present, no transgenic sugarcane has been released to the commercial market, but with the aid of large investments from the private sector, the commercialization of this major sugar-and biomass-producing crop should be accelerated.

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“…without callus formation) in vitro was first reported by Irvine and Benda (32) during a process of rapid regeneration of plantlets in an attempt to eliminate sugarcane of SCMV. It was recently reported again during studies on suspension cultures for protoplast isolation (46), and in a transformation protocol using leaf discs as target material for bombardment, with plantlet regeneration occurring via embryo formation directly on leaf discs (47)(48)(49). Although this approach in a transformation programme could substantially reduce the length of time in culture and avoid prolonged exposure to high levels of 2,4-D, the technique is currently unsuitable for widespread adoption.…”

Section: An Overview Of In Vitro Culture Systems and Transformation Tmentioning

confidence: 99%

Sugarcane Transformation

Snyman

2004

Transgenic Crops of the World

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A Comparison of Direct and Indirect Somatic Morphogenesis for the Production of Transgenic Sugarcane (Saccharum Spp. Hybrids)

Cited by 22 publications

References 0 publications

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

Genetic Engineering of Saccharum

Sugarcane Transformation

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