A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic wounds

Park, Soon‐Jung; Moon, Sung-Hwan; Lee, Hye‐Jin; Lim, Joa-Jin; Kim, Jung-Mo; Seo, Joseph; Yoo, Ji-Woon; Kim, Ok-Jung; Kang, Sun-Woong; Chung, Hyung-Min

doi:10.1016/j.biomaterials.2012.10.039

Cited by 33 publications

(28 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The iPS-EPCs generated in the present study display phenotypic and functional traits consistent with previous findings [8,9,11,19,25,26]. CD34 + /CD133 + /Flk1 + cells are defined as EPCs [3,4].…”

Section: Discussionsupporting

confidence: 77%

“…Pluripotent stem cells, such as embryonic stem (ES) cells, can be expanded indefinitely in culture and have the remarkable ability to generate different cell types, including endothelial lineage cells [7][8][9][10][11], in virtually unlimited numbers. In particular, mouse embryonic EPCs are able to target lung metastases in an allogeneic setting in mice [6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antitumor Effects of CD40 Ligand-Expressing Endothelial Progenitor Cells Derived From Human Induced Pluripotent Stem Cells in a Metastatic Breast Cancer Model

Purwanti

Chen

Lam

et al. 2014

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Given their intrinsic ability to home to tumor sites, endothelial progenitor cells (EPCs) are attractive as cellular vehicles for targeted cancer gene therapy. However, collecting sufficient EPCs is one of the challenging issues critical for effective clinical translation of this new approach. In this study, we sought to explore whether human induced pluripotent stem (iPS) cells could be used as a reliable and accessible cell source to generate human EPCs suitable for cancer treatment. We used an embryoid body formation method to derive CD133 + CD34+ EPCs from human iPS cells. The generated EPCs expressed endothelial markers such as CD31, Flk1, and vascular endothelial-cadherin without expression of the CD45 hematopoietic marker. After intravenous injection, the iPS cell-derived EPCs migrated toward orthotopic and lung metastatic tumors in the mouse 4T1 breast cancer model but did not promote tumor growth and metastasis. To investigate their therapeutic potential, the EPCs were transduced with baculovirus encoding the potent T cell costimulatory molecule CD40 ligand. The systemic injection of the CD40 ligand-expressing EPCs stimulated the secretion of both tumor necrosis factor-a and interferon-g and increased the caspase 3/7 activity in the lungs with metastatic tumors, leading to prolonged survival of the tumor bearing mice. Therefore, our findings suggest that human iPS cell-derived EPCs have the potential to serve as tumor-targeted cellular vehicles for anticancer gene therapy. STEM CELLS TRANSLATIONAL MEDICINE 2014;3:923-935

show abstract

Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Antitumor Effects of CD40 Ligand-Expressing Endothelial Progenitor Cells Derived From Human Induced Pluripotent Stem Cells in a Metastatic Breast Cancer Model

Purwanti

Chen

Lam

et al. 2014

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…Therefore, we examined whether H‐CM treatment had beneficial effects on the skin wound‐healing process. To accomplish this, we conducted an in vitro cell migration assay (37) and an in vivo skin wound‐healing analysis (38, 39). H‐CM treatment enhanced cellular migration in a dose‐dependent manner (Fig.…”

Section: Resultsmentioning

confidence: 99%

Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway

et al. 2015

View full text Add to dashboard Cite

Cellular senescence, an irreversible state of growth arrest, underlies organismal aging and agerelated diseases. Recent evidence suggests that aging intervention based on inhibition of cellular senescence might be a promising strategy for treatment of aging and age-related diseases. Embryonic stem cells (ESCs) and ESC conditioned medium (CM) have been suggested as a desirable source for regenerative medicine. However, effects of ESC-CM on cellular senescence remain to be determined. We found that treatment of senescent human dermal fibroblasts with CM from mouse ESCs (mESCs) decreases senescence phenotypes. We found that plateletderived growth factor BB in mESC-CM plays a critical role in antisenescence effect of mESC-CM through upregulation of fibroblast growth factor 2. We confirmed that mESC-CM treatment accelerates the wound-healing process by down-regulating senescence-associated p53 expression in in vivo models. Taken together, our results suggest that mESC-CM has the ability to suppress cellular senescence and maintain proliferative capacity. Therefore, this strategy might emerge as a novel therapeutic strategy for aging and age-related

show abstract

“…Lee et al (16) subcutaneously injected stem cells into the topical skin at the wound edge and ischemic lower limb of diabetic mice and identified that fibroblast growth factor, vascular endothelial growth factor (VEGF), lower limb perfusion and capillary density in the local skin of the mice were significantly higher than those in diabetic mice that were not injected with stem cells, and that the skin healing rate was significantly accelerated. Park et al (17) reported that stem cells promoted cell proliferation, cell migration towards the wound area and angiogenesis. Furthermore, Shin and Peterson (18) indicated that after the wound margin in DM mice was transplanted with stem cells, the levels of VEGF and platelet-derived growth factor, which facilitate the repair of skin tissues, were significantly increased, and mobilization of the host's own stem cells surrounding the wound edge was also increased.…”

Section: Efficacy Safety and Influencing Factors Of Intra-calf Muscumentioning

confidence: 99%

Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease

et al. 2017

View full text Add to dashboard Cite

A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic wounds

Cited by 33 publications

References 29 publications

Antitumor Effects of CD40 Ligand-Expressing Endothelial Progenitor Cells Derived From Human Induced Pluripotent Stem Cells in a Metastatic Breast Cancer Model

Antitumor Effects of CD40 Ligand-Expressing Endothelial Progenitor Cells Derived From Human Induced Pluripotent Stem Cells in a Metastatic Breast Cancer Model

Antisenescence effect of mouse embryonic stem cell conditioned medium through a PDGF/FGF pathway

Efficacy, safety and influencing factors of intra‑calf muscular injection of bone marrow mononuclear cells in the treatment of type 2 diabetes mellitus‑induced lower extremity vascular disease

Contact Info

Product

Resources

About