A comparison of in vitro cytotoxicity assays in medical device regulatory studies

Liu, Xuemei; Rodeheaver, D.; White, Jeffrey C.; Wright, Ann; Walker, Lisa M.; Zhang, Fan; Shannon, Stephen Paul

doi:10.1016/j.yrtph.2018.06.003

Cited by 98 publications

(54 citation statements)

References 75 publications

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“…Many cytotoxic experiments have been carried out in vitro following the traditional approaches created for soluble nanoparticles [ 76 ]. In these conditions, nanomaterials tend to aggregate and sediment.…”

Section: Resultsmentioning

confidence: 99%

Anti-Candidal Activity and In Vitro Cytotoxicity Assessment of Graphene Nanoplatelets Decorated with Zinc Oxide Nanorods

et al. 2018

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Candida albicans is the most common pathogenic fungus that is isolated in nosocomial infections in medically and immune-compromised patients. The ability of C. albicans to convert its form from yeast to hyphal morphology contributes to biofilm development that effectively shelters Candida against the action of antifungals molecules. In the last years, nanocomposites are the most promising solutions against drug-resistant microorganisms. The aim of this study was to investigate the antifungal activity of graphene nanoplateles decorated with zinc oxide nanorods (ZNGs) against the human pathogen Candida albicans. We observed that ZNGs were able to induce a significant mortality in fungal cells, as well as to affect the main virulence factors of this fungus or rather the hyphal development and biofilm formation. Reactive Oxygen Species (ROS) formation in yeast cells resulted one of the mechanisms of ZNGs to induce mortality. Finally, the toxicity of this nanomaterial was tested also on human keratinocyte cell line HaCaT. Our data indicated that ZNGs resulted not toxic when their aggregation state decreased by adding glycerol as emulsifier to ZNGs suspensions or when HaCaT cells were grown on ZNGs-coated glasses. Overall, the results that were obtained indicated that ZNGs could be exploited as an antifungal nanomaterial with a high degree of biocompatibility on human cells.

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Section: Resultsmentioning

confidence: 99%

Anti-Candidal Activity and In Vitro Cytotoxicity Assessment of Graphene Nanoplatelets Decorated with Zinc Oxide Nanorods

et al. 2018

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“…It does not measure the physical or mechanical impact because the test material is not in direct contact with the cells. 130 Danion et al, investigated the potential toxicity of the extracted solution from a so contact lens coat with liposomes on the epithelial cells. 129 They incubated the lens on the culture media, which was then used for the growth of the cells without involving direct contact of the lens and calculated the cell viability by XTT assay, which suggested that no cytotoxic compounds were present in the extract solution.…”

Section: Evaluation Of Drug Laden Contact Lensmentioning

confidence: 99%

“…Aer 24-72 hours of the incubation period, the cytotoxicity effect can be evaluated. 130,131 The free material from the contact lens has the potential to penetrate the cell and interact with DNA. Comet assay is a microgel electrophoresis technique for measuring DNA damage and DNA repair.…”

Section: Evaluation Of Drug Laden Contact Lensmentioning

confidence: 99%

Advances in chemistry and composition of soft materials for drug releasing contact lenses

et al. 2020

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“…The biocompatibility test of gelatin film was performed according to ISO 10993 [41] by MTT assay using L929 fibroblast cells. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was used to evaluate the cell viability based on the mitochondrial conversion of the tetrazolium salt into a purple colored formazan product at an absorbance of 570 nm.…”

Section: Cell Biocompatibility Assaymentioning

confidence: 99%

A Facile Fabrication of Biodegradable and Biocompatible Cross-Linked Gelatin as Screen Printing Substrates

et al. 2020

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This study focuses on preparation and valuation of the biodegradable, native, and modified gelatin film as screen-printing substrates. Modified gelatin film was prepared by crosslinking with various crosslinking agents and the electrode array was designed by screen-printing. It was observed that the swelling ratio of C-2, crosslinked with glutaraldehyde and EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide) was found to be lower (3.98%) than that of C-1 (crosslinked with only glutaraldehyde) (8.77%) and C-0 (without crosslinking) (28.15%). The obtained results indicate that the swelling ratios of both C-1 and C-2 were found to be lower than that of C-0 (control one without crosslinking). The Young’s modulus for C-1 and C-2 was found to be 8.55 ± 0.57 and 23.72 ± 2.04 kPa, respectively. Hence, it was conveyed that the mechanical strength of C-2 was found to be two times higher than that of C-l, suggesting that the mechanical strength was enhanced upon dual crosslinking in this study also. The adhesion study indicates that silver ink adhesion on the gelation surface is better than that of carbon ink. In addition, the electrical response of C-2 with a screen-printed electrode (SPE) was found to be the same as the commercial polycarbonate (PC) substrate. The result of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay suggested that the silver SPE on C-2 was non-cytotoxic toward L929 fibroblast cells proliferation. The results indicated that C-2 gelatin is a promising material to act as a screen-printing substrate with excellent biodegradable and biocompatible properties.

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A comparison of in vitro cytotoxicity assays in medical device regulatory studies

Cited by 98 publications

References 75 publications

Anti-Candidal Activity and In Vitro Cytotoxicity Assessment of Graphene Nanoplatelets Decorated with Zinc Oxide Nanorods

Anti-Candidal Activity and In Vitro Cytotoxicity Assessment of Graphene Nanoplatelets Decorated with Zinc Oxide Nanorods

Advances in chemistry and composition of soft materials for drug releasing contact lenses

A Facile Fabrication of Biodegradable and Biocompatible Cross-Linked Gelatin as Screen Printing Substrates

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