A Comparison of Media Supplement Methods for the Extended Culture of Human Islet Tissue1

Dw, Fraga; Sabek, Omaima; Dk, Hathaway; Gaber, A. Osama

doi:10.1097/00007890-199804270-00009

Cited by 81 publications

(37 citation statements)

References 12 publications

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“…In a different study, coculture with ductal epithelial cells helped maintain structural integrity and viability of the islets (Ilieva et al, 1999). Fraga et al (1998) have evaluated different media supplements for the extended culture of human pancreatic islets. Islet culture in Connaught Medical Research Laboratories medium (Life Technologies, Inc., Rockville, MD) was compared with the supplementation of either 10% fetal bovine serum (standard medium) or insulin-, transferrin-, and selenium-containing medium (also known as the Memphis medium).…”

Section: A Loss Of Islet Viability During Isolation and Culturementioning

confidence: 99%

Biological and Biomaterial Approaches for Improved Islet Transplantation

2006

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Section: A Loss Of Islet Viability During Isolation and Culturementioning

confidence: 99%

Biological and Biomaterial Approaches for Improved Islet Transplantation

2006

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“…Our previous work has resulted in improved recovery and viability of human islets by optimization of islet culture conditions. 5,6 Islet destruction following transplantation can be prevented by genetically engineering b-cells to (1) promote revascularization, and intercept (2) apoptotic and inflammatory pathways by expressing desired proteins in the vicinity of the islets. [7][8][9] IL-10 gene expression appears to provide significant improvements in islet function in either aspect and prevents type I diabetes, 10 although simultaneous expression of both IL-4 and IL-10 has been shown to be more effective in this clinical setting.…”

Section: Introductionmentioning

confidence: 99%

Adenovirus-based vascular endothelial growth factor gene delivery to human pancreatic islets

et al. 2004

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Islet transplantation is limited by islet graft failure due to poor revascularization, host immune rejection and nonspecific inflammatory response. Delivery of human vascular endothelial growth factor (hVEGF) gene to the islets is likely to promote islet revascularization and survival. We used a bicistronic adenoviral vector encoding hVEGF and CpG-free allele of green fluorescent protein (Adv-GFP-hVEGF) and introduced into human pancreatic islets by transfection. We found that transfection efficiency and apoptosis were dependent on the multiplicity of infection (MOI). Compared to Adv-GFP transfected and nontransfected islets, the levels of hVEGF secreted from Adv-GFP-hVEGF transfected islets were higher and exhibit a linear relationship between hVEGF expression and MOI (10-5000). Persistent, but low level expression of hVEGF from nontransfected islets was also observed. This may be due to expression of the endogenous hVEGF gene under hypoxic conditions. The levels of DNA fragmentation determined by ELISA of islet lysates were dependent on the MOI of Adv-GFP-hVEGF. On glucose challenge, insulin release from transfected islets was comparable to nontransfected islets. Immunohistochemical staining for hVEGF was very high in Adv-GFP-hVEGF transfected islets. Weak staining was also observed for hCD31 in both transfected and nontransfected islets. These findings suggest that Adv-GFP-hVEGF is a potential candidate for promoting islet revascularization.

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“…26,27 In the many case of the preserved islet, one major disadvantage of islet culture is the loss of tissue mass that occurs over time. 28 The ET-K preservation method demonstrated here allows tissue ATP synthesis and amelioration of cold ischemic tissues damage during extended 24 h isolated-islet preservation. Donor pancreas or isolated islets are usually preserved with UW solution.…”

Section: Discussionmentioning

confidence: 96%

“…However, one major disadvantage of islet culture is the loss of tissue mass that occurs over time. 11 Technical and immunological challenges, however, remain prior to larger scale, cost-effective application of islet transplantation. Technical challenges remain, including a limited human islet supply from cadavers, a low islet yield and quality associated with donor brain death, and long cold-ischemia times during organ procurement, storage and transportation.…”

Section: Introductionmentioning

confidence: 99%