A Comparison of Performances of Four Enzymes Used in Elisa with Special Reference to β-Lactamase

Khatkhatay, M. Ikram; Desai, Meena

doi:10.1080/01971529909349349

Cited by 20 publications

(11 citation statements)

References 59 publications

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“…While the 96-well carbon sensor microplate device has been designed for use with HRP, it was here used for an immunoassay using alkaline phosphatase as marker enzyme because, from the electrochemical point of view, the latter gives a better signal than HRP (Khatkhatay and Desai, 1999). Before running the study to optimise the immunoassay parameters, amperometric experiments were carried out in order to evaluate the optimal conditions for measurement of the enzymatic activity of AP.…”

Section: Mei Parametersmentioning

confidence: 99%

Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection

Piermarini

Micheli

Ammida

et al. 2007

Biosensors and Bioelectronics

183

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Section: Mei Parametersmentioning

confidence: 99%

Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection

Piermarini

Micheli

Ammida

et al. 2007

Biosensors and Bioelectronics

183

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“…28,[81][82][83] In this study, GAL monoclonal antibody (mAB) and hydrophobic autocovariance eigenvector template designed peptides target the dominant eigenmodes of GAL directly. Binding of GAL by targeted antibodies (see above), mAB 84,85 or landscape phage-derived peptides 33,35 activates GAL's enzymatic activity with respect to its substrate, Onitrophenyl--galactoside (ONPG).…”

Section: The Results Of Two Types Of Screening Experimentsmentioning

confidence: 99%

“…81,83,88 The ELx 808 Ultra Microplate Reader (Bio-Tek Instruments, Winooski, VT) and the Sanbrook 89 96-well plate, colorimetric, spectrophotometric measurement of GAL's product were used in these studies. The reader was controlled and data captured by an external PC running KC4 Data Reduction Software (Bio-Tek Instruments, Winooski, VT).…”

Section: The Results Of Two Types Of Screening Experimentsmentioning

confidence: 99%

Designing allosteric peptide ligands targeting a globular protein

et al. 2006

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Patented signal analytic algorithms applied to hydrophobically transformed, numerical amino acid sequences have previously been used to design short, protein‐targeted, L or D retro‐inverso peptides. These peptides have demonstrated allosteric and/or indirect agonist effects on a variety of G‐protein and tyrosine kinase coupled membrane receptors with 30% to over 80% hit rates. Here we extend these approaches to a globular protein target. We designed eight peptide ligands targeting an ELISA antibody responsive protein, β‐galactosidase, βGAL. Three of the eight 14mer peptides allosterically activated βGAL with ELISA methodology. Using Bayesian statistics, this 38% hit rate would have occurred 2 × 10−9 by chance. These peptides demonstrated binding site competitive or noncompetitive interactions, suggesting allosteric site multiplicity with respect to their βGAL binding‐mediated ELISA signal. Kinetic studies demonstrated the temperature dependence of the βGAL peptide binding functions. Using the van't Hoff relation, we found evidence for enthalpy–entropy compensation. This relation is often found for hydrophobic interactions in aqueous media, and is consistent with the postulated hydrophobic series encoding underlying our protein‐targeted, peptide design methods. It appears that our algorithmic, hydrophobic autocovariance eigenvector template approach to the design of allosteric peptides targeting membrane receptors may also be applicable to the design of peptide ligands targeting nonmembrane involved globular proteins. © 2006 Wiley Periodicals, Inc. Biopolymers 85: 38–59, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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“…In situations of a high-level endogenous peroxidase activity, AlkP becomes a more suitable choice. Among other frequently used enzymes is β-D-galactosidase (β-gal), whose bioconjugates are much more stable and can be stored for at least one year at 4 °C [ 114 ]. However, due to its high molecular weight and low turnover number, β-gal is used less.…”

Section: Immunoassays and Aptamer-based Assays With Optical Detectmentioning

confidence: 99%

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Jamal

Shipovskov

Ferapontova

2020

Sensors

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Microbiological safety of the human environment and health needs advanced monitoring tools both for the specific detection of bacteria in complex biological matrices, often in the presence of excessive amounts of other bacterial species, and for bacteria quantification at a single cell level. Here, we discuss the existing electrochemical approaches for bacterial analysis that are based on the biospecific recognition of whole bacterial cells. Perspectives of such assays applications as emergency-use biosensors for quick analysis of trace levels of bacteria by minimally trained personnel are argued.

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A Comparison of Performances of Four Enzymes Used in Elisa with Special Reference to β-Lactamase

Cited by 20 publications

References 59 publications

Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection

Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection

Designing allosteric peptide ligands targeting a globular protein

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

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