A comparison of plate assay methods for detecting extracellular cellulase and xylanase activity

Meddeb-Mouelhi, Fatma; Moisan, Jessica Kelly; Beauregard, Marc

doi:10.1016/j.enzmictec.2014.07.004

Cited by 103 publications

(72 citation statements)

References 12 publications

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“…The Congo red staining method is reliable because it avoids the identification of false positives, and it has been used for decades. 26 The Gyx086 strain exhibited the biggest (D/d) 2 value (6.10 ± 0.13), which suggested that this strain possessed the highest hydrolysis ability. The A. niger Gyx086 was therefore preliminarily selected for the next part of the experiment.…”

Section: Resultsmentioning

confidence: 90%

“…Nevertheless, A. niger isolates have significantly different hydrolysis ability, with (D/d) 2 values ranging from 1.43 ± 0.36 to 6.10 ± 0.13. The Congo red staining method is reliable because it avoids the identification of false positives, and it has been used for decades . The Gyx086 strain exhibited the biggest (D/d) 2 value (6.10 ± 0.13), which suggested that this strain possessed the highest hydrolysis ability.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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A low‐cost recycling system for the production of pectinase was built to improve the yield and quality of Muzao date juice and to reduce production costs. An Aspergillus niger strain, Gyx086, was selected from 20 A. niger strains on potato agar plates containing Muzao date residues, and the strain was used to produce an enzyme cocktail with high pectinase activity using Muzao date residues as the medium. The high pectinase activity level was therefore used in the production of Muzao date juice. Under optimized enzymatic hydrolysis conditions, a date juice yield of 92.88% was obtained, which was 11% higher than the control, and was not significantly different from a commercial preparation. Further storage trials indicated that the clarity, viscosity, storage stability, and soluble solid content could also be greatly improved by enzymatic treatment. These results suggested that the cheap experimental preparation could effectively replace the commercial preparation used for the production of date juice. This is the first systematic report detailing the production of Muzao date juice using a cheap and effective pectinase cocktail, which could promote the development of the date juice industry. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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“…Plate clearing assays were performed using Congo Red indicator as described by Meddeb-Mouehli et al, in which 4 gauge mycelial pads from spore suspension in HGLM were inoculated onto Carboxymethylcellulose (CMC)/Sorbose agar (1X Vogel’s salts, 0.5% CMC, 2% Sorbose, 1.5% Agar) and plates were flooded 3 days post-inoculation with 0.1% Congo Red for 20 min, and washed sequentially with 1 M NaCl for 15 min [23]. All samples were performed in biological triplicates.…”

Section: Methodsmentioning

confidence: 99%

Developing elite Neurospora crassa strains for cellulosic ethanol production using fungal breeding

Waters

Nixon

Dwyer

et al. 2017

Journal of Industrial Microbiology and Biotechnology

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The demand for renewable and sustainable energy has generated considerable interest in the conversion of cellulosic biomass into liquid fuels such as ethanol using a filamentous fungus. While attempts have been made to study cellulose metabolism through the use of knock-out mutants, there have been no systematic effort to characterize natural variation for cellulose metabolism in ecotypes adapted to different habitats. Here, we characterized natural variation in saccharification of cellulose and fermentation in 73 ecotypes and 89 laboratory strains of the model fungus Neurospora crassa. We observed significant variation in both traits among natural and laboratory generated populations, with some elite strains performing better than the reference strain. In the F1 population N345, 15% of the population outperformed both parents with the top performing strain having 10% improvement in ethanol production. These results suggest that natural alleles can be exploited through fungal breeding for developing elite industrial strains for bioethanol production.

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“…The reagent solution was decanted, and the plates were again flooded with NaCl (1 M) for another 20 min. Clear zones around the fungal colony indicated that the production of enzymes is positive [36,41].…”

Section: Enzymes Production By the Fungi Inactivated By Sc-comentioning

confidence: 99%

“…The inactivation process was conducted at the lethal condition of SC-CO 2 (35 MPa, 75 • C, 90 min) and autoclave (15 psi, 121 • C and 20 min) using plate assay technique. The production of extracellular enzymes was detected based on the presence or absence of clearing zone around the grown colony on the solid media as mentioned by Hankin and Anagnostakis [31], Rajamani and Hilda [32], Khokhar et al [33], Morris et al [34], Gopinath et al [35] and MeddebMouelhi et al [36]. Tween 20 agar medium containing ingredients below in L −1 ; peptone, 10 g; CaCl 2 · 2H 2 O, 0.1 g; NaCl, 5.0 g; agar, 20 g and 10 mL of Tween 20 was used for bioassay of lipase [37].…”

Section: Enzymes Production By the Fungi Inactivated By Sc-comentioning

confidence: 99%

Inactivation of Aspergillus Spores in Clinical Wastes by Supercritical Carbon Dioxide

Efaq

Rahman²,

Nagao³

et al. 2016

Arab J Sci Eng

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The present work investigated the inactivation of Aspergillus spp. spores by supercritical carbon dioxide (SC-CO 2 ) based on the destruction of enzyme activity and the alteration of spore morphology as documented using a scanning electron microscope (SEM). Six Aspergillus spp. included A. niger, Aspergillus sp. in section Nigri, A. fumigatus, A. tubingensis, A. hortai and Aspergillus sp. strain no 145 were isolated from clinical waste samples and inactivated by using SC-CO 2 at optimal conditions (35 MPa, 75 • C and 90 min). The inactivation of spores was determined by the culturing method. Aspergillus sp. strain no 145 and Aspergillus hortai were further analysed to quantify enzyme activity, and the morphology of spores was determined by SEM to demonstrate the inactivation mechanism. The results revealed that growth and enzyme activity of the SC-CO 2 treated fungal spores were not detected. The SEM observation demonstrated complete destruction of spore morphology and exsorption of protoplasmic contents. The findings proved the high efficiency of SC-CO 2 for the destruction of fungal contaminants in clinical wastes. B A. N. Efaq

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A comparison of plate assay methods for detecting extracellular cellulase and xylanase activity

Cited by 103 publications

References 12 publications

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Developing elite Neurospora crassa strains for cellulosic ethanol production using fungal breeding

Inactivation of Aspergillus Spores in Clinical Wastes by Supercritical Carbon Dioxide

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