A Comparison of Silica C and Silica Gel in HILIC Mode: The Effect of Stationary Phase Surface Area

Santali, Eman Y.; Edwards, Darren; Sutcliffe, Oliver B.; Bailes, Sophie; Euerby, Melvin R.; Watson, David

doi:10.1007/s10337-014-2694-9

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…Chromatographic conditions such as stationary phase (column), mobile phase composition (solvent ratio, pH, salt type, its concentration), and temperature have significant effects on the chromatography behavior of polar compounds under HILIC . Type B silica (Atlantis HILIC Silica) and type C silica (Cogent Silica‐C column) columns were investigated to compare the retention of ME because it has been found previously that type silica C showed higher retention than type B silica column, particularly for basic compounds .…”

Section: Resultsmentioning

confidence: 99%

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Douša

Pivoňková

Sýkora

2016

J of Separation Science

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A rapid procedure for the determination of memantine based on hydrophilic interaction chromatography with fluorescence detection was developed. Fluorescence detection after postcolumn derivatization with o-phtaldialdehyde/2-mercaptoethanol was performed at excitation and emission wavelengths of 345 and 450 nm, respectively. The postcolumn reaction conditions such as reaction temperature, derivatization reagent flow rate, and reagents concentration were studied due to steric hindrance of amino group of memantine. The derivatization reaction was applied for the hydrophilic interaction liquid chromatography method which was based on Cogent Silica-C stationary phase with a mobile phase consisting of a mixture of 10 mmol/L citric acid and 10 mmol/L o-phosphoric acid (pH 6.0) with acetonitrile using an isocratic composition of 2:8 v/v. The benefit of the reported approach consists in a simple sample pretreatment and a quick and sensitive hydrophilic interaction chromatography method. The developed method was validated in terms of linearity, accuracy, precision, and selectivity according to the International Conference on Harmonisation guidelines. The developed method was successfully applied for the analysis of commercial memantine tablets.

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Section: Resultsmentioning

confidence: 99%

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Douša

Pivoňková

Sýkora

2016

J of Separation Science

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“…The Silica-C stationary phase has also been proven to be successful in the aqueous normal phase mode [55,56]. A number of pharmaceutical compounds have been analyzed using this stationary phase by ANP.…”

Section: Silica Cmentioning

confidence: 99%

Silica Hydride: A Separation Material Every Analyst Should Know About

Pesek

Matyska

2021

Molecules

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This review describes the development, special features and applications of silica hydride-based stationary phases for HPLC. The unique surface of this material is in contrast to ordinary, standard silica, which is the material most frequently used in modern HPLC stationary phases. The standard silica surface contains mainly silanol (Si-OH) groups, while the silica hydride surface is instead composed of silicon-hydrogen groups, which is much more stable, less reactive and delivers different chromatographic and chemical characteristics. Other aspects of this material are described for each of the different bonded moieties available commercially. Some applications for each of these column types are also presented as well as a generic model for method development on silica hydride-based stationary phases.

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“…When developing robust methods in HILIC, it is important to understand the dominant mechanism that governs retention which is related to the physicochemical characteristics of the analytes and to the analytical column substrate. The basic types of HILIC columns include plain silica, neutral, polar chemically bonded, ion-exchange and zwitterionic stationary phases [19,20]. The retention mechanism in HILIC chromatography is complex involving hydrophilic partition, absorption and electrostatic interactions and further studies are needed to elucidate this [21][22][23].…”

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confidence: 99%

Investigation of the Retention Mechanism of Cephalosporins by Zwitterionic Hydrophilic Interaction Liquid Chromatography

Panderi¹,

Malamos²,

Machairas³

et al. 2016

Chromatographia

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adverse effects on human health, such as resistance development and allergic reactions [1][2][3][4]. There is no doubt that the importance of cephalosporin antibiotics in medicine requires the development of high performance separation methods for their analysis [5,6]. Literature survey revealed that numerous chromatographic methods have been developed for the analysis of cephalosporins including isocratic or gradient techniques coupled with various methods of detection [7][8][9][10][11][12][13][14]. These compounds are highly polar molecules and represent perfect candidates to study the retention mechanism in hydrophilic interaction liquid chromatography (HILIC). Up to now research on the retention of cephalosporins in HILIC has been focused only on the use of plain silica columns [15,16]. The zwitterionic hydrophilic interaction liquid chromatography used in this study is a unique form of HILIC which involves the use of stationary phases with zwitterionic functional groups covalently bonded at a silica or polymeric substrate.HILIC chromatography is an alternative chromatographic technique using polar stationary phases and low aqueous/high organic mobile phases in order to achieve effective retention for polar analytes. It appears to be complementary to reversed-phase LC as the order of elution is reversed to reversed-phase LC and thus it provides reasonable retention for polar compounds [17,18]. When developing robust methods in HILIC, it is important to understand the dominant mechanism that governs retention which is related to the physicochemical characteristics of the analytes and to the analytical column substrate. The basic types of HILIC columns include plain silica, neutral, polar chemically bonded, ion-exchange and zwitterionic stationary phases [19,20]. The retention mechanism in HILIC chromatography is complex involving hydrophilic partition, absorption and electrostatic interactions and further studies are needed to elucidate this [21][22][23]. AbstractThe importance of cephalosporin antibiotics in medicine requires the development of high performance separation methods for their analysis. In this paper cefazolin, cefuroxime, cefoxitin, cefaclor and ceftriaxone were used as model compounds to investigate the retention mechanism under zwitterionic HILIC conditions. The analysis was performed using a ZIC ® -HILIC analytical column (150.0 × 2.1 mm i.d., particle size 3.5 μm, 200 Å) with isocratic elution. The mobile phase was a mixture of acetonitrile and ammonium acetate or formate buffer. To get a better insight into the retention mechanism on ZIC ® -HILIC columns the main factors influencing the retention and selectivity of the analytes have been thoroughly investigated. It is shown that the type of the salt and its concentration along with the volume fraction of water in the mobile phase and pH of the aqueous buffer greatly affect the retention of the tested cephalosporins due to their structures and physicochemical properties. The method was applied successfully to the analysis of injection dosage...

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A Comparison of Silica C and Silica Gel in HILIC Mode: The Effect of Stationary Phase Surface Area

Cited by 9 publications

References 17 publications

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Optimization of o‐phtaldialdehyde/2‐mercaptoethanol postcolumn reaction for the hydrophilic interaction liquid chromatography determination of memantine utilizing a silica hydride stationary phase

Silica Hydride: A Separation Material Every Analyst Should Know About

Investigation of the Retention Mechanism of Cephalosporins by Zwitterionic Hydrophilic Interaction Liquid Chromatography

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