A Comparison of Stool Enteropathogen Detection by Semiquantitative PCR in Adults With Acute Travelers’ Diarrhea Before and 3 Weeks After Successful Antibiotic Treatment

Tisdale, Michele; Tribble, David R.; Telu, Kalyani; Fraser, Jamie; Connor, Patrick; Philip, Cliff; Odundo, Elizabeth; Reyes, Faviola; Simons, Mark P.; Swierczewski, Brett E.; Lizewski, Stephen E.; Liu, Jie; Houpt, Eric R.; Riddle, Mark S.; Lalani, Tahaniyat

doi:10.1093/ofid/ofz187

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…Despite the established link between TD and post-infectious IBS (PIIBS), recent studies have shed light on its prevalence and potential mechanism involving the continuous activation of the immune system in PI-IBS and other persistent non-IBS abdominal issues (PI-AC). In a prospective study conducted in 2018, a group of 101 participants were surveyed regarding their gastrointestinal symptoms before and after traveling, with follow-up intervals at 2 weeks, 6 months, and 1 year [81]. While the overall rates of PI-IBS and PI-AC were relatively low, individuals who experienced TD had a higher incidence of PI-AC (though not PI-IBS) compared to those who did not.…”

Section: Post-infectious Chronic Gastrointestinal Disordersmentioning

confidence: 99%

Recent Advances in Travelers’ Diarrhea: Epidemiology, Diagnosis, and Treatment

Atluri,

Raghukumar,

Sreenath

2024

Prensa Med Argent

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and the Middle East being at the greatest risk [5]. The precise epidemiology of TD is not well comprehended. The incidence is higher in individuals visiting countries with limited resources, but the specific rates vary depending on the location and time of year of travel [6]. The cause of diarrhea is determined by the geographical area, food hygiene standards, sanitation practices, water supply, and the season. In more than half of individuals with diarrhea, no specific pathogens are identified. When it comes to returning travelers, approximately 50% of cases are caused by bacteria such as enterotoxigenic Escherichia coli, Salmonella, Shigella, Campylobacter, Vibrio, Yersinia, and Aeromonas [7]. MethodsComparing certain reports published between 2000 and 2018 containing the terms 'traveler's diarrhea' (including variations such as travelers and diarrhoea

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Section: Post-infectious Chronic Gastrointestinal Disordersmentioning

confidence: 99%

Recent Advances in Travelers’ Diarrhea: Epidemiology, Diagnosis, and Treatment

Atluri,

Raghukumar,

Sreenath

2024

Prensa Med Argent

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“…Polymerase Chain Reaction (PCR) assays have signi cantly improved the detection of travelers' diarrhea (TD) pathogens but interpreting results is often challenging due to asymptomatic shedding and multipathogen detection [1][2][3]. Studies evaluating the performance characteristics of PCR assays have largely been conducted in clinic or hospital settings using diarrheal samples tested within days of collection [4,5].…”

Section: Introductionmentioning

confidence: 99%

Performance characteristics of a quantitative PCR assay on repository stool specimens and smeared filter-paper cards

Tisdale

Mitra

McCoy

et al. 2020

Preprint

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Objective Stool repositories are a valuable resource for retrospective analyses including quantitative PCR assays to distinguish between asymptomatic shedding and clinical disease. The suitability of archival specimens for this purpose is unclear and requires assessment. We conducted a pilot study to evaluate pathogen detection by TaqMan Array Card (TAC) in travelers’ diarrhea (TD) stool specimens stored for 1-13 years, as well as the impact of transporting specimens on Whatman FTA Elute cards (FTA Cards) on detection. Results The positive percent agreement (PPA) for TAC on stool vs. microbiologic testing was lower than our a priori PPA estimate of 80% for most pathogens: Shigella spp. (100% [95%CI: 69-100%]), enterotoxigenic E coli (ETEC) (63% [95%CI: 49-75%]), Campylobacter spp. (66% [95%CI: 43-85%]) and Norovirus (37% [95%CI: 16-61%]). Use of the FTA card resulted in a further reduction of PPA. Our findings suggest that archival specimens may lead to insensitive detection on quantitative PCR assays due to degradation of nucleic acid with prolonged storage, although our limited sample size precluded us from evaluating the impact of storage duration on nucleic acid yield. Additional studies are needed to understand the impact of storage duration on quantitative PCR data.

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Travel, Diarrhea, Antibiotics, Antimicrobial Resistance and Practice Guidelines—a Holistic Approach to a Health Conundrum

Riddle

2020

Curr Infect Dis Rep

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A Comparison of Stool Enteropathogen Detection by Semiquantitative PCR in Adults With Acute Travelers’ Diarrhea Before and 3 Weeks After Successful Antibiotic Treatment

Cited by 8 publications

References 12 publications

Recent Advances in Travelers’ Diarrhea: Epidemiology, Diagnosis, and Treatment

Recent Advances in Travelers’ Diarrhea: Epidemiology, Diagnosis, and Treatment

Performance characteristics of a quantitative PCR assay on repository stool specimens and smeared filter-paper cards

Travel, Diarrhea, Antibiotics, Antimicrobial Resistance and Practice Guidelines—a Holistic Approach to a Health Conundrum

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