A comparison of supramammillary and medial septal influences on hippocampal field potentials and single-unit activity

Abstract

SUMMARYAND CONCLUSIONS 1. A comparison was made between the influences of supramammillary (SUM) and medial septal (MS) nuclei on hippocampal physiology in Nembutal-anesthetized rats. Specifically, the effects of prestimulation of the SUM or MS on the perforant path-dentate field potential, on spontaneous activity of single units, and on perforant path-induced unit activation were assessed. Another series of experiments addressed the issue of whether the SUM and MS effects on the perforant path-dentate field response are independent.2. Prestimulation of the SUM or MS significantly facilitated the perforant path-dentate population spike with no clear effect on the field excitatory postsynaptic potential (EPSP) recorded in the subgranular zone of the dentate hilus. Prestimulation of either nucleus also reduced the threshold for spike onset. The major differences between the two spike facilitation effects were the magnitude of the change and possibly the optimal interstimulus intervals required to obtain the effects.3. Acute transection of the ipsilateral column of fornix or dorsal fornix eliminated the SUM population spike facilitation effect. MS lesion or dorsal fornix/fimbria transection eliminated the MS spike facilitation effect. The MS lesion did not alter the effects of SUM prestimulation. Cingulum or medial forebrain bundle transection affected neither SUM-nor MS-mediated spike facilitation. Thus the SUM and MS influences on the dentate field response appear to be independent of one another. The relevant SUM afferents travel through the ipsilateral column of fornix and dorsal fornix, whereas MS afferents project through the dorsal fornix/fimbria.4. Single units recorded in stratum granulosum (SG) were assessed with respect to several parameters. These included the mean firing rate, whether or not excitation occurred prior to the field population spike and at lower threshold, and whether or not a driven unit responded to a second perforant path stimulus delivered at short latency following the first (during the period of population spike depression). The latter parameter in particular appeared to separate SG cells into two classes. The cells that were not activated during the second field potential were classified as granule cells, whereas those that were activated were classified as basket cells. Based on this distinction, significant differences were also found between the two cell classes on the other parameters. In particular, cells classified as granule cells often had very low firing rates. We conclude that many previous studies have mistakenly identified as granule cells inhibitory interneurons, which are much more commonly encountered (at least partly due to their higher discharge rates). This misidentification has led to several hypotheses concerning the mechanism of heterosynaptically induced population spike facilitation that we now conclude are untenable.5. Stimulation of the SUM or MS alone resulted in a reduction in the spontaneous firing rate of more than one-half of the cells recorded in the S...

Differential responses of hippocampal subfields to cortical up–down states

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“…Therefore, S can be treated as an array of Boolean variables. The integration time for inhibitory interneurons, however, is smaller than for principal cells (Fox and Ranck, 1981;McNaughton and Morris, 1987;Mizumori et al, 1989;Shepherd, 1990) and is close to the time bin. For this reason, the approximation of fast inhibition is used, assuming that the amount of inhibition (uniformly distributed among all units) is adjusted at every discrete time bin, so that the total number M of firing units is preserved near the given level, which varies periodically in time according to the theta rhythm.…”

Long-Term Recordings Improve the Detection of Weak Excitatory–Excitatory Connections in Rat Prefrontal Cortex

“…However, those are embedded in a much larger matrix of weak connections, which are difficult to capture with extracellular recordings that only detect action potential transmission. With regard to successful spike transmission, pyramidal-interneuron pairs have been shown to be more reliable (Mizumori et al, 1989;Marshall et al, 2002;Holmgren et al, 2003;Swadlow, 2003). Using a less stringent detection threshold, such as 1%, as used in many previous studies, is therefore less likely to be severely affected by false-positives.…”