A comparison of the distribution of central cholinergic neurons as demonstrated by acetylcholinesterase pharmacohistochemistry and choline acetyltransferase immunohistochemistry

Organization of choline acetyltransferase-containing structures in the forebrain of the rat

“…The distribution of ChAT-positive perikarya in the olfactory tubercle demonstrated in the present study is in agreement with that observed by others in the rat Kimura et al, 1981b;Satoh et al, 1983;Sofroniew et al, 1982). The presence of ChAT-positive terminal-like structures in the olfactory tubercle and pyriform cortex has been reported by Kimura et al (198 la, 1983) and Chao et al (1982).…”

“…Basal forebrain Our observation on the morphology and topographical localization of ChAT-positive perikarya is similar to those in previous studies on the rat Houser et al, 1983;Kimura et al, 1981b;Satoh et al, 1983: Sofroniew et al, 1982, and is compatible with those on the cat (Kimura et al, 1981a), primate (Hedreen et al, 1983;Mesulam et al, 1984), and human (Nagai et al, 1983). We found no ChAT-positive structure in the neuropil, although Kimura et al (1981a) reported that ChAT-positive dots are attached to the surfaces of ChAT-positive and -negative perikarya in the cat.…”

“…While several investigators observed ChAT-positive neurons in the cortex (Eckenstein and Bat&man, 1984;Eckenstein and Thoenen, 1983;Houser et al, 1983), others failed to demonstrate neurons with ChAT-positivity Kimura et al, 1981aKimura et al, , b, 1983Mesulam et al, 1984;Satoh et al, 1983;Sofroniew et al, 1982). Our observation corroborates the presence of cholinergic neuronal perikarya in the cortex.…”

“…The distribution of the cholinergic perikarya in the mammalian CNS has been established immunohistochemically using antisera (Kimura et al, 198 la, b;Sofroniew et al, 1982) or monoclonal antibodies Hedreen et al, 1983;Houser et al, 1983;Mesulam et al, 1984;Nagai et al, 1983;Satoh et al, 1983) to ChAT. ChAT immunohistochemistry has revealed that the histochemical demonstration of AChE, which has been widely used for studying cholinergic neurons, can be adopted only in some regions in the CNS (Eckenstein and Sofroniew, 1983;Levey et al, 1983;Mesulam et al, 1984;Satoh et al, 1983).…”

“…The distribution of the cholinergic perikarya in the mammalian CNS has been established immunohistochemically using antisera (Kimura et al, 198 la, b;Sofroniew et al, 1982) or monoclonal antibodies Hedreen et al, 1983;Houser et al, 1983;Mesulam et al, 1984;Nagai et al, 1983;Satoh et al, 1983) to ChAT. ChAT immunohistochemistry has revealed that the histochemical demonstration of AChE, which has been widely used for studying cholinergic neurons, can be adopted only in some regions in the CNS (Eckenstein and Sofroniew, 1983;Levey et al, 1983;Mesulam et al, 1984;Satoh et al, 1983). Substantial evidence concerning the ascending cholinergic pathways in the CNS has also been derived from the immunohistochemical studies of ChAT in combination with tracer transport (Mesulam et al, 1983a, b;Nagai et al, 1982;Saper, 1984;Wahle et al, 1984;Wainer and Rye, 1984;Woolf et al, 1983).…”