A comparison of the effect of various topically active steroids on the clinical and histological features of psoriasis

Goodwin, Peter; Hamilton, Susan; Fry, Lionel

doi:10.1111/j.1365-2133.1973.tb01918.x

Cited by 8 publications

(4 citation statements)

References 6 publications

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“…Thus, retinoids block the formation of a normal stratum corneum and induce numerous extra vital cell layers in upper epidermis (Tammi and Tammi, 1986). Pharmacological doses of glucocorticoids, on the other hand, enhance normal keratinocyte differentiation and reduce the number of vital cell layers, as shown in cultured epidermis (Tammi and Tammi, 1986) and in the successful treatment of many skin diseases with disordered keratinization (Fry and McMinn, 1968;Goodwin et al, 1973). Glucocorticoids may even play a specific role in the induction of keratinization Endo, 1969, 1971;Obinata and Endo, 1977).…”

Section: Hc Control Of Epidermal Hyaluronanmentioning

confidence: 99%

Hydrocortisone regulation of hyaluronan metabolism in human skin organ culture

Ågren

Tammi

1995

Journal Cellular Physiology

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We studied the influence of hydrocortisone (HC) on hyaluronan (HA) metabolism in explants of human skin, a model retaining normal three-dimensional architecture of dermal connective tissue and dynamic growth and stratification of epidermal keratinocytes. The synthesis of hyaluronan and proteoglycans (PGs), and DNA, were determined with 3H-glucosamine and 3H-thymidine labelings, respectively. The total content and histological distribution of hyaluronan was studied utilizing a biotinylated aggrecan-link protein complex. A low concentration of HC (10(-9) M) stimulated the incorporation of 3H-glucosamine into hyaluronan in epidermis by 23% and reduced the disappearance rate of hyaluronan by 25% in chase experiments, resulting in a 74% increase in total hyaluronan (per epidermal dry weight) after a 5-day culture in 10(-9) M HC. On the other hand, a high concentration of HC (10(-5) M) reduced both synthesis (-42%) and degradation (-46%) of epidermal hyaluronan during 24 h labeling and chase periods. The cumulative effect of a 5-day treatment was a 24% decrease of total epidermal hyaluronan. The high dose (10(-5) M) also reduced keratinocyte DNA synthesis and epidermal thickness. In dermis, only the high (10(-5) M) concentration of HC was effective, inhibiting the incorporation of 3H-glucosamine into hyaluronan by 28%. No significant influences on total hyaluronan content or the disappearance rate of hyaluronan in dermal tissue was found. All HC concentrations lacked significant effects on newly synthesized PGs in epidermal and dermal tissues, but reduced the labeled PGs diffusing into culture medium. A low physiological concentration of HC thus maintains active synthesis and high concentration of hyaluronan in epidermal tissue, while high pharmacological doses of HC slows hyaluronan turnover and reduces its content in epidermis, an effect correlated with enhanced terminal differentiation, reduced proliferation rate and reduced number of vital keratinocyte layers.

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Section: Hc Control Of Epidermal Hyaluronanmentioning

confidence: 99%

Hydrocortisone regulation of hyaluronan metabolism in human skin organ culture

Ågren

Tammi

1995

Journal Cellular Physiology

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“…The bio-availabilities and activities of many proprietary topical corticosteroid formulations were assessed by the single-application occluded vasoconstrictor test by Barry & Woodford (1974, I975> 1976. That work permitted rank order classification of the preparations and often demonstrated comparability between the results obtained and those reported in clinical trials (Portnoy, 1972;Baran, 1972;Rex, 1973;Goodwin, Hamilton & Fry, 1973;MacDonald & Fry, 1974;Sparkes & Wilson, 1974;Whitefield & McKenzie, 1975;Corbett, 1976;Wilson, 1976). However, in the treatment of dermatological disease a steroid preparation is not usually applied once only under occlusion but may be rubbed into the non-occluded affected area, often several times a day.…”

mentioning

confidence: 73%

Vasoconstrictor activities and bio-availabilities of seven proprietary corticosteroid creams assessed using a non-occluded multiple dosage regimen;clinical implications

Barry

Woodford

1977

Br J Dermatol

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The bio-availabilities and activities of commercial creams (Metosyn, Synalar, Betnovate, Locoid, Alphaderm, Dioderm and Efcortelan 1%) were assessed using a multiple dosage regimen vasoconstrictor assay in ten volunteers. The blanching response first increased but then diminished with continued application over a 5-day period: following a 2-day 'resting period', when no steroid was applied, the application sites recovered considerably but tachyphylaxis again resulted from subsequent repeated applications. Acute tolerance occurred with the hydrocortisone formulations (Alphaderm, Dioderm and Efcortelan 1%) in a manner similar to that observed with the potent preparations. Metosyn was significantly more active (P less than 0.05), and Efcortelan 1% was significantly less active (P less than 0.05), than the other formulations. Apart from the reversal of Dioderm and Alphaderm the rank order of preparations was similar to that previously noted in the 6 h occluded single-application vasoconstrictor test. The results may have significance in dosage from design and application for topical corticosteroid formulations.

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“…Evidence for a basic defect in the skin derives from studies of epidermal proliferation in psoriatic plaques [23,24]. Transplantation and recombinant grafting studies in nude mice have implicated components of both the epidermis and dermis [25, 261.…”

Section: Discussionmentioning

confidence: 99%

A study of protein synthesis in cells cultured from involved psoriatic skin

Easty

Patel²,

Otto

et al. 1991

Electrophoresis

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Using histochemical techniques an abnormal programme of epidermal differentiation has been well documented in psoriasis. In order to characterise further the biochemistry of this process we have cultured dermal fibroblasts and epidermal keratinocytes from involved psoriatic skin. This has facilitated metabolic radiolabelling of skin cells and analysis of protein synthesis by two-dimensional polyacrylamide gel electrophoresis. The expression of keratin and differentiation markers was identical to that of normal keratinocytes, suggesting that psoriatic epidermal differentiation is not truncated in vitro as has been postulated to be the case in vivo. Low molecular mass components (5-8.5 kDa), previously shown to be upregulated in suprabasal keratinocytes, were detected in epidermal fractions from psoriatic skin enriched for basal cells. Of special interest was a component of 26 kDa, pI 5.9, which was highly upregulated in psoriatic as compared to normal cultured keratinocytes and was not detected in fibroblasts. These findings are in accord with a qualitatively abnormal pattern of differentiation for keratinocytes in the involved psoriatic epidermis.

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A comparison of the effect of various topically active steroids on the clinical and histological features of psoriasis

Cited by 8 publications

References 6 publications

Hydrocortisone regulation of hyaluronan metabolism in human skin organ culture

Hydrocortisone regulation of hyaluronan metabolism in human skin organ culture

Vasoconstrictor activities and bio-availabilities of seven proprietary corticosteroid creams assessed using a non-occluded multiple dosage regimen;clinical implications

A study of protein synthesis in cells cultured from involved psoriatic skin

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