A Comparison of the Effects of Estradiol and 2- and 4-Hydroxyestradiol on Uterine Ornithine Decarboxylase Activity in Immature Rats

Kuroda, Hiroshi; Johnson, Donald C.; Dey, Sudhansu K.; Takeo, Satoshi

doi:10.1254/jjp.61.65

Cited by 9 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In laboratory studies, individual estrogen metabolites have been shown to exhibit important differences in biological activity that may differentially impact breast cancer risk. For example, the 4-hydroxy and 16α-hydroxy metabolites are relatively more estrogenic than the 2-hydroxy metabolites (4,5). In addition, the 4-hydroxy metabolites may form DNA adducts that can lead to mutations that initiate breast tumors (6).…”

Section: Introductionmentioning

confidence: 99%

Effect of Flaxseed Consumption on Urinary Levels of Estrogen Metabolites in Postmenopausal Women

Sturgeon

Volpe

Puleo

et al. 2010

Nutrition and Cancer

View full text Add to dashboard Cite

Flaxseed is a rich source of dietary lignans. It has been hypothesized that lignans may decrease breast cancer risk through modulation of endogenous hormone levels. The aim of this study was to determine the effect of flaxseed supplementation on urinary levels of estrogen metabolites that may be involved in the development of breast cancer. Forty-three postmenopausal women participated in this 12-wk preintervention-postintervention study. Participants consumed 7.5 g/day of ground flaxseed for 6 wk, followed by 15 g/day for an additional 6 wk. The mean urinary level of 16alpha -hydroxyestrone (16alpha -OHE1) was higher at the end of 12 wk compared to baseline (change of 1.32 ug/day, P = 0.02). There was no significant change in 2-OHE1 excretion. The mean urinary level of the 2-OHE1/16alpha -OHE1 ratio was lower at the end of 12 wk compared to baseline (change of -1.1, P = 0.02). Mean urinary excretion of 2-methoxyestradiol was also lower at 12 wk than at baseline (P = 0.03). Based on the current paradigm of the effects of estrogen metabolism on breast cancer risk, the regimen of dietary flaxseed intake used in this study did not appear to favorably alter breast cancer risk through shifts in estrogen metabolism pathways in postmenopausal women.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Flaxseed Consumption on Urinary Levels of Estrogen Metabolites in Postmenopausal Women

Sturgeon

Volpe

Puleo

et al. 2010

Nutrition and Cancer

View full text Add to dashboard Cite

show abstract

“…The rapid growth and differentiation of uterus are concomitant with increased expression of the ornithine decarboxylase gene. Recent studies have shown that expression of ornithine decarboxylase gene in the uterus is augmented by EDs [45,46,47,48]. These estrogen-sensitive genes have therefore been used as markers for evaluating the estrogenic potential of EDs in the uterus…”

Section: Biomarkers For Measuring the Estrogenic Effect Of Edsmentioning

confidence: 99%

Biomarker Genes for Detecting Estrogenic Activity of Endocrine Disruptors via Estrogen Receptors

Jung

Yang

et al. 2012

IJERPH

View full text Add to dashboard Cite

Endocrine disruptors (EDs) are compounds used in various industrial products, drugs, and cosmetics. They can be found in the environment and disturb the endocrine and reproductive systems, resulting in adverse effects to humans and wildlife such as birth defects and developmental disorders. Since several EDs have a structure similar to that of endogenous steroid hormones such as estrogens, they intend to have an affinity for steroid hormone receptors and alter hormone-mediated metabolism by binding to these receptors. EDs are therefore a global concern and assays should be developed to efficiently determine whether these compounds are detrimental to biological systems. Diverse experimental methods may help determine the endocrine disrupting potential of EDs and evaluate the adverse effects of a single and/or combination of these reagents. Currently, biomarkers have been employed to objectively measure EDs potency and understand the underlying mechanisms. Further studies are required to develop ideal screening methods and biomarkers to determine EDs potency at environmentally relevant concentrations. In this review, we describe the biomarkers for estrogenicity of EDs identified both in vitro and in vivo, and introduce a biomarker, cabindin-D9k (CaBP-9k), that may be used to assess estrogenic activity of EDs.

show abstract

Effect of Oaz1 overexpression on goose ovarian granulosa cells

Kang

Jiang

et al. 2017

Amino Acids

View full text Add to dashboard Cite

Ornithine decarboxylase antizyme 1 (OAZ1) regulates both polyamine biosynthesis and transport and acts as a tumor suppressor because of its functions in the regulation of cell proliferation, apoptosis and growth. However, the roles of OAZ1 in the regulation of polyamine metabolism, cell proliferation, apoptosis and hormone receptor expression in granulosa cells (GCs) from geese have not been determined. To define the roles of OAZ1 in primary GCs, we constructed and transfected an Oaz1 overexpression vector with a thymidine deletion into primary GCs. Oaz1 overexpression induced 65 and 30% decreases in the putrescine and spermidine contents, respectively, 225% increase in the spermine content and up-regulated the expression levels of genes associated with polyamine metabolism (but Oaz2). The Ccnd1, Pcna, Bax and Caspase 3 mRNA expression levels in GCs overexpressing Oaz1 were significantly higher than the expression levels in the vehicle group (p < 0.05). Additionally, a slight increase in the E2 concentration in the culture medium and enhanced Er, Fshr and Lhr expression were observed in the GCs 24 h after Oaz1 overexpression (p < 0.05). These data suggested that Oaz1 up-regulated the expression of genes related to polyamine metabolic enzymes and reproductive hormone receptors, disturbed intracellular polyamine homeostasis, and affected the transcription of genes associated to cell proliferation and apoptosis in goose primary GCs. Taken together, our results indicate that modifying polyamine metabolism by Oaz1 may interfere with the expression of genes involved in cell proliferation, apoptosis and responsiveness of ovary toward hormones in goose GCs.

show abstract

A Comparison of the Effects of Estradiol and 2- and 4-Hydroxyestradiol on Uterine Ornithine Decarboxylase Activity in Immature Rats

Cited by 9 publications

References 13 publications

Effect of Flaxseed Consumption on Urinary Levels of Estrogen Metabolites in Postmenopausal Women

Effect of Flaxseed Consumption on Urinary Levels of Estrogen Metabolites in Postmenopausal Women

Biomarker Genes for Detecting Estrogenic Activity of Endocrine Disruptors via Estrogen Receptors

Effect of Oaz1 overexpression on goose ovarian granulosa cells

Contact Info

Product

Resources

About