A comparison of the FECPAK and Mini-FLOTAC faecal egg counting techniques

Godber, Olivia Florence; Phythian, Clare J; Bosco, Antonio; Ianniello, Davide; Coles, G.C.; Rinaldi, Laura; Cringoli, Giuseppe

doi:10.1016/j.vetpar.2014.12.029

Cited by 40 publications

(35 citation statements)

References 10 publications

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“…Faecal egg counts were performed on dung samples from a commercial herd of 60 organic cattle in south‐west England to confirm natural infection, using the Mini‐FLOTAC ® method, which is accurate to five eggs per gram (Godber et al . ). None of the animals had been treated with anthelmintics for at least the previous 6 months.…”

Section: Methodsmentioning

confidence: 97%

Dung beetles reduce livestock gastrointestinal parasite availability on pasture

Sands

Wall²

2016

Journal of Applied Ecology

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Summary Anthelmintics are widely used to control gastrointestinal parasites of livestock. However, the residues of these compounds, particularly the macrocyclic lactones, are excreted largely unmetabolised in faeces, where they may have toxic effects on dung‐colonising insects. Impoverishment of the coprophagous beetle community impairs the process of dung recycling and, as a result, may enhance the persistence of dung‐dwelling helminth parasitic stages. To test this possibility, a large‐scale field trial was conducted in south‐west England. The availability of infective parasite helminth larvae (L3) was investigated on the herbage around 240 artificial 1‐kg dung pats that had been constructed from the faeces of beef cattle with naturally acquired strongyle infections. Herbage up to 15 cm surrounding each pat was sampled at 2, 4, 6, 8 and 10 weeks after deposition. Pats were subject to enhanced, natural or no dung beetle colonisation and uncontrolled or enhanced rainfall. Under uncontrolled rainfall conditions, 2 weeks after pat deposition, significantly more L3 were recovered from around pats that were exposed to beetle colonisation than from pats that were not colonised. However, by week 8, significantly fewer L3 were recovered from around pats that were exposed to beetle colonisation compared to uncolonised pats. Under conditions of enhanced rainfall, pats yielded significantly more L3 than under uncontrolled rainfall conditions, and there were no differences in recovery from herbage around pats with enhanced, natural or no beetle colonisation. The data suggest that over the duration of a summer grazing season, temperate habitat dung‐colonising insect communities, which include mainly small endocoprid dung beetles of the genus Aphodius, can reduce the development and survival of livestock gastrointestinal parasites on pastures, but that this can be overridden by the effect of high rainfall. Synthesis and applications. The work demonstrates that conservation of dung beetle populations in temperate climates is important in livestock management, not only for their essential role in dung degradation and nutrient cycling, but because their activity can also reduce the survival and availability of gastrointestinal parasites on pastures.

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Section: Methodsmentioning

confidence: 97%

Dung beetles reduce livestock gastrointestinal parasite availability on pasture

Sands

Wall²

2016

Journal of Applied Ecology

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“…Meanwhile updated guidelines for the performance and evaluation of FECRT trials are made available, the outlined limitations of the test need to be addressed. For example in cattle, where FEC are usually lower than in sheep, the use of more sensitive egg counting methods such as FLOTAC and Mini-FLOTAC could be evaluated (Levecke et al 2012b;Godber et al 2015;Cringoli et al 2017). In cases where AR is detected, further studies can look at the direct impact of infection with resistance parasites (leading to inefficacious treatments) on animal health and farm productivity, as earlier reported (Sutherland et al 2010, Miller et al 2012, Borges et al 2013.…”

Section: Perspectives and Further Researchmentioning

confidence: 99%

Drug resistance in parasitic helminths of veterinary importance in Chile: Status review and research needs

Pena-Espinoza

2018

Austral j. vet. sci.

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The increasing development of anthelmintic resistance (AR) in parasites of livestock is threatening animal health and production worldwide. In Chile, studies evaluating the field efficacy of anthelmintics and the detection of AR have been performed since the 2000s, but until now, no previous attempt has tried to systematise the available information. This article reviews general concepts about AR in helminths of veterinary importance, methods for diagnosis of AR and summarises the published reports of AR in Chile. Anthelmintic resistance in Chile has been reported in gastrointestinal nematodes of horses (benzimidazole resistance) and ruminants (sheep and cattle, macrocyclic lactone and benzimidazole resistance). However, these cases involved a limited number of selected farms and no further conclusions can be made of the status of parasite drug resistance at a regional or national level. No published cases of AR in Fasciola hepatica have been reported in Chilean livestock, but human infections with triclabendazole-resistant F. hepatica have been described in patients with previous consumption of watercress or untreated water from marshes grazed by livestock. Given the zoonotic potential and endemic nature of F. hepatica in Chile, it is urgent to determine the extent of liver fluke resistance. Current research gaps of the situation of AR in Chile and suggestions for the performance of laboratory and field studies are further discussed.

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“…It was realized with an alternative tool that stocks parasitic forms without centrifugal step (Mini-FLOTAC, Silva et al, 2013;Godber et al, 2015), using a floatation solution (specific gravity 1.3).…”

Section: Examined Samplesmentioning

confidence: 99%

Telediagnosis: Parasitological experiences in wild ruminants of South African preserves

Zaffarano¹,

Morandi²,

Menegotto³

et al. 2018

J. Vet. Med. Anim. Health

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A survey on wild ruminants' health status of any South African preserves was attempted, assessing body condition score (BCS) through tele-diagnosis. The wildlife BCS was linked to the presence of gastrointestinal parasites that should be recognized, counted and statistically evaluated. For this purpose, we examined 103 faecal samples of wild ruminants from 6 South African preserves. For practical reasons, the animals were divided into two macro-categories: small and large ruminants. The results obtained showed a prevalence of 78.1 and 15.6% in large ruminants for gastrointestinal strongyles (GIS) and coccidian, respectively, while small ruminants showed 92.3% due to GIS and 30.8% for coccidia. No statistically significant difference in the prevalence among the preserves was detected; on the other hand, a low value of BCS corresponds to a greater presence of parasites with statistics difference in the macro-categories (small ruminant x 2 =5.238; P=0.020; large ruminant x 2 = 15.215; P<0.001) and sex classes (male x 2 =5.409; P=0.020; female x 2 =17.350; P<0.001). For these reasons, our results provide a practical feedback for the management preserves. The present paper is fully part of the limited experiences of telediagnosis in a conservation perspective. Based on the results obtained, we decided to organize a project that could limit and assess the risk factors in the management of these activities in the South African context.

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A comparison of the FECPAK and Mini-FLOTAC faecal egg counting techniques

Cited by 40 publications

References 10 publications

Dung beetles reduce livestock gastrointestinal parasite availability on pasture

Dung beetles reduce livestock gastrointestinal parasite availability on pasture

Drug resistance in parasitic helminths of veterinary importance in Chile: Status review and research needs

Telediagnosis: Parasitological experiences in wild ruminants of South African preserves

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